A5101744899- Immunotherapy and Immune Responses
- T-cell and B-cell Immunology
- Autophagy in Disease and Therapy
- Autoimmune Bullous Skin Diseases
- Immune Cell Function and Interaction
- Endoplasmic Reticulum Stress and Disease
- Dermatology and Skin Diseases
- Urticaria and Related Conditions
- Contact Dermatitis and Allergies
- Cervical Cancer and HPV Research
- Nonmelanoma Skin Cancer Studies
- Neuroendocrine Tumor Research Advances
- Heat shock proteins research
- CAR-T cell therapy research
- Cell Adhesion Molecules Research
- Lipid metabolism and biosynthesis
- Immunodeficiency and Autoimmune Disorders
- Immune Response and Inflammation
- Fungal Infections and Studies
- Molecular Biology Techniques and Applications
- Toxoplasma gondii Research Studies
- Nail Diseases and Treatments
- Cancer and Skin Lesions
- Ion channel regulation and function
- Cutaneous lymphoproliferative disorders research
Johannes Gutenberg University Mainz
2018-2023
University Medical Center of the Johannes Gutenberg University Mainz
2018-2023
Hôpital Edouard Herriot
1977-2003
Inserm
1984-2003
Centre National de la Recherche Scientifique
1991-2001
Université Claude Bernard Lyon 1
1993
Clinique Ambroise Paré
1993
Universitäts Hautklinik Kiel
1982
Resource10 October 2022Open Access Transparent process Lipid and protein content profiling of isolated native autophagic vesicles Daniel Schmitt The Autophagy Lab, Institute Pathobiochemistry, University Medical Center the Johannes Gutenberg University, Mainz, Germany Contribution: Conceptualization, Data curation, Formal analysis, Validation, Investigation, Writing - review & editing Search for more papers by this author Süleyman Bozkurt orcid.org/0000-0003-4965-3195 Biochemistry II,...
Abstract Most monocyte-derived dendritic cells (DC) display CD1a, like Langerhans (LC) and some dermal DC, but their relationship with these skin DC remains unclear. To address this issue, we studied the expression of different antigens characteristic monocyte/macrophages in CD1a+ CD1a– DC. Their phenotype indicated that they may be related to rather than LC, i.e., were all CD11b-positive, 72% Factor XIIIa-positive, did not express E-cadherin nor VLA-6. It is interesting showed...
Autophagy is dependent on appropriate lipid supply for autophagosome formation. The regulation of acquisition and the autophagy network response to lipid-limiting conditions are mostly elusive. Here, we show that knockout RAB GTPase RAB18 interferes with droplet catabolism, causing an impaired fatty acid release. resulting reduced lipid-droplet-derived availability influences provokes adaptive modifications network. These adjustments include increased expression phosphorylation ATG2B as well...
Contact sensitivity to para-phenylenediamine (PPD) is a frequent delayed-type hypersensitivity resulting in contact dermatitis. The aim of the present study, conducted 16 patients allergic PPD (as assessed by positive patch test), was get better insight into mechanism T-cell activation sensitivity. unable induce significant proliferation T cells from first set 9 patients. In 7 further patients, lymphocyte using and 2 metabolites, namely Bandrowski's base (BB) benzoquinone (BQ). BB...
Sugar receptors are being increasingly implicated in host-pathogen interactions because of their specific recognition carbohydrates microorganisms. The aim this study was to identify sugar expressed on the surface human epidermal Langerhans cells (LC). To end, binding a panel fluorescent neoglycoproteins LC analyzed by quantitative flow cytofluorometry after standardization with calibrated beads. We demonstrate that fresh only isolated from healthy epidermis which express membrane receptor...
In the present study, we analyzed mechanism by which human Langerhans cells (LC), dendritic (DC) from epidermis, support induction of a primary allogeneic T cell response. We reported that paraformaldehyde (PF) fixation completely abrogated stimulatory property freshly isolated LC, although level major histocompatibility complex (MHC) class II antigen (Ag) expression was unaltered fixative. Addition either interleukin (IL)-1 beta and/or IL-6, during mixed epidermal lymphocyte reaction,...
SUMMARY Aspergillus fumigatus is the most common aetiological fungus responsible for human pulmonary aspergilloses. This study investigated primary contact between Langerhans cells (LC), corresponding to dendritic present in mucosa and live conidia of A. fumigatus. LC play a key role antigen presentation initiation T cell response. In vitro-generated (iLC) were differentiated from cultured cord blood CD34+ incubated at 4°C or 37°C with fluorescein-isothiocyanate (FITC)-stained control latex...
Somatostatin (SOM) is a ubiquitous peptide which responsible for the inhibition of numerous biological functions. SOM described as an antiproliferative molecule and inhibitor exocrine or endocrine secretion from variety tissues, including pancreas, gastrointestinal tract, central peripheral nervous system. Mediation effects can be indirect direct, respectively, through other molecules receptors on target cells. We have searched presence in epidermis using immunofluorescence, confocal laser...
Langerhans cells originate in bone marrow and probably belong to the monocyte-macrophage lineage. CD1 is a specific marker of cells. By immunofluorescence immunoelectron microscopy, CD1a antigen myeloid markers (CD11, CD13, CD14, CD15, CD33, HLA-DR) were studied 53 cases acute leukemias (AML) 3 lymphoblastic (ALL). The 11 ANLL without monocytic component negative. 2/5 myelomonocytic (AML4) 9/37 (AML5) positive. All ALL No correlation was found between markers. CD1a+ AML did not differ from...
Abstract: All three‐dimensional in vitro mucosal models constructed, thus far, have only been reconstituted by epithelial cells. We developed a reconstructed oral and vaginal epithelium that integrates Langerhans' cells (LC), the dendritic (DC) of malpighian epithelia. The was composed gingival or keratinocytes seeded on de‐epidermized dermis (DED) grown submerged culture for 2 weeks. LC precursors, obtained after differentiation cord blood‐derived CD34 + hematopoietic progenitor (CD34 HPC)...
Abstract Autophagy is a central eukaryotic catabolic pathway responsible for clearance and recycling of an extensive portfolio cargoes, which are packed in vesicles, called autophagosomes, delivered to lysosomes degradation. Besides basal autophagy, constantly degrades cellular material, the highly responsive several stress conditions. However, exact protein content phospholipid composition autophagosomes under changing autophagy conditions remain elusive so far. Here, we introduce...
Abstract The co‐chaperone BAG3 is a hub for variety of cellular pathways via its multiple domains and interaction with chaperones the HSP70 family or small HSPs. During aging under stress conditions in particular, BAG3, together molecular chaperones, ensures sequestration aggregated aggregation‐prone ubiquitinated proteins to autophagic‐lysosomal system ubiquitin receptors. Accumulating evidence BAG3‐mediated selective autophagy independent cargo ubiquitination led analyses predicting direct...