A5046821096- Advanced Proteomics Techniques and Applications
- Cardiovascular Function and Risk Factors
- Pancreatic function and diabetes
- Nitric Oxide and Endothelin Effects
- Metabolism, Diabetes, and Cancer
- Metabolomics and Mass Spectrometry Studies
- Mitochondrial Function and Pathology
- Adipose Tissue and Metabolism
- Diabetes Treatment and Management
- Blood Pressure and Hypertension Studies
- Atherosclerosis and Cardiovascular Diseases
- Protein Hydrolysis and Bioactive Peptides
- Pulmonary Hypertension Research and Treatments
- Mass Spectrometry Techniques and Applications
- Diabetes and associated disorders
- Peroxisome Proliferator-Activated Receptors
- Cancer, Hypoxia, and Metabolism
- Diet, Metabolism, and Disease
- Peptidase Inhibition and Analysis
- Heart Failure Treatment and Management
- Chronic Kidney Disease and Diabetes
- Adipokines, Inflammation, and Metabolic Diseases
- Genetics, Aging, and Longevity in Model Organisms
- Dialysis and Renal Disease Management
- Cardiovascular Disease and Adiposity
University Medical Center of the Johannes Gutenberg University Mainz
2019-2025
Johannes Gutenberg University Mainz
2019-2025
German Centre for Cardiovascular Research
2021-2023
Mosaiques Diagnostics and Therapeutics (Germany)
2013-2017
KU Leuven
2015-2016
Navarre Institute of Health Research
2016
University of Glasgow
2015-2016
Universidad de Navarra
2016
Maastricht University
2015
Scania (Sweden)
2011-2013
Idiopathic pulmonary arterial hypertension (IPAH) is pathogenetically related to low levels of the vasodilator nitric oxide (NO). Because NO regulates cellular respiration and mitochondrial biogenesis, we hypothesized that abnormalities bioenergetics may be present in IPAH. Evaluation artery endothelial cells from IPAH control lungs vitro revealed oxygen consumption was decreased, especially state 3 with substrates glutamate-malate or succinate, this decrease paralleled reduction Complex IV...
Chronic kidney disease (CKD) is part of a number systemic and renal diseases may reach epidemic proportions over the next decade. Efforts have been made to improve diagnosis management CKD. We hypothesised that combining metabolomic proteomic approaches could generate more complete view mechanisms. To test this approach, we examined samples from cohort 49 patients representing different stages Urine were analysed for changes using capillary electrophoresis-mass spectrometry urine plasma mass...
A cellular prooxidant state promotes cells to neoplastic growth, in part because of modification proteins and their functions. Reactive nitrogen species formed from nitric oxide (NO) or its metabolites, can lead protein tyrosine nitration, which is elevated lung cancer.To determine the alteration these NO derivatives role they may play contributing carcinogenesis.We analyzed levels NO, nitrite (NO2-), nitrate (NO3-), location nitration identified that are modified.Although exhaled NO2- were...
The biochemical mechanisms underlying glucose-stimulated insulin secretion from pancreatic beta-cells are not completely understood. To identify metabolic disturbances in that impair secretion, we compared two INS-1-derived clonal beta-cell lines, which glucose-responsive (832/13 cells) or glucose-unresponsive (832/2 cells). this end, analyzed a number of parameters glycolytic and mitochondrial metabolism, including mRNA expression genes involved cellular energy metabolism. We found despite...
Insulin secretion is coupled with changes in β-cell metabolism. To define this process, 195 putative metabolites, mitochondrial respiration, NADP+, NADPH and insulin were measured within 15 min of stimulation clonal INS-1 832/13 β-cells glucose. Rapid responses the major metabolic pathways glucose occurred, involving several previously suggested coupling factors. The complexity metabolite observed disagreed concept one single controlling secretion. complex alterations levels suggest that a...
BackgroundWe investigate whether the urinary proteome refines diagnosis of renal dysfunction, which affects over 10% adult population.
Heart failure is a multifaceted clinical syndrome, in which the heart fails to supply adequate blood meet body's oxygen and nutrients needs. Evidence indicates multi-level molecular shifts subjects, necessitating unbiased stratification of patients with failure. This study utilized AI-based multimodal integration method analyse 359 lipids 538 proteins measured participants MyoVasc cohort. Patient similarity networks were constructed, spectral clustering, an unsupervised machine learning...
<title>Abstract</title> The emergence of post-COVID sequelae (PCS) represents a global challenge. However, understanding biological mechanisms and the definition quantifiable risk factors remains limited. This study harnessed power predictive machine learning models to explore potential proteomics in predicting individual symptoms their collective manifestation as PCS. analysis utilized panel approximately 2900 proteins measured 495 COVID-19 patients. identified 235 unique associated with 21...
Aging affects oxidative metabolism in liver and other tissues. Carnitine acyltransferases are key enzymes of this process mitochondria. As previously shown, the rate transcription activity carnitine palmitoyltransferase CPT1 also related to levels. In study we compared effect dietary L-carnitine (100 mg L-carnitine/kg body weight/day over 3 months) on aged rats (months 21–24) adult animals 6–9) age-related controls for both groups. The CPT1, CPT2, acetyltransferase (CRAT) was determined by...
BackgroundIn previous studies, we identified two urinary proteomic classifiers, termed HF1 and HF2, which discriminated subclinical diastolic left ventricular (LV) dysfunction from normal.HF1 HF2 combine information 85 671 peptides, mainly up-or down-regulated collagen fragments.We sought to validate these classifiers in a population study. MethodsIn 745 people randomly recruited Flemish (49.8 years; 51.3% women), measured early late peak velocities of mitral inflow (E A) annular (e' a') by...
Background Biomarker discovery and new insights into the pathophysiology of heart failure with reduced ejection fraction (HFrEF) may emerge from recent advances in high-throughput urinary proteomics. This could lead to improved diagnosis, risk stratification management HFrEF. Methods Results Urine samples were analyzed by on-line capillary electrophoresis coupled electrospray ionization micro time-of-flight mass spectrometry (CE-MS) generate individual proteome profiles. In an initial...
In a previous cross-sectional study, we identified multidimensional urinary classifier (HF1), which was associated with left ventricular dysfunction. We investigated whether HF1 predicts cardiovascular end points over and beyond traditional risk factors. 791 randomly recruited Flemish (mean age, 51.2 years; 50.6% women), quantified by capillary electrophoresis coupled mass spectrometry. addition, measured averaged −0.97 U (range, −3.26 to 2.60). Over 6.1 years (median), 35 participants died...
Abstract Background and Aims Chronic inflammation autoimmunity contribute to cardiovascular (CV) disease. Recently, autoantibodies (aAbs) against the CXC-motif-chemokine receptor 3 (CXCR3), a G protein-coupled with key role in atherosclerosis, have been identified. The of anti-CXCR3 aAbs for CV risk disease is unclear. Methods Anti-CXCR3 were quantified by commercially available enzyme-linked immunosorbent assay 5000 participants (availability: 97.1%) population-based Gutenberg Health Study...
Protein tyrosine nitration increases in vivo as a result of oxidative stress and is elevated numerous inflammatory-associated diseases. Mammalian fructose-1,6-bisphosphate aldolases are nitrated lung epithelial cells liver, well retina under different inflammatory conditions. Using two-dimensional gel electrophoresis matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, we now show that aldolase A human skin fibroblasts. To reveal the consequences nitration, studied...
Insulin secretion from pancreatic β-cells is controlled by complex metabolic and energetic changes provoked exposure to fuels. Perturbations in these processes lead impaired insulin secretion, the ultimate cause of T2D (Type 2 diabetes). To increase our understanding stimulus-secretion coupling potentially involved pathogenesis T2D, a comprehensive investigation response glucose-responsive INS-1 832/13 glucose-unresponsive 832/2 β-cell lines was performed. For this metabolomics analysis, we...
WNT5A has been identified as an important ligand in the malignant progression of a number tumours. Although signalling is often altered cancer, ligand's role either tumour suppressor or oncogene varies between types and contemporary issue for investigators β-catenin-independent WNT oncology. Here, we report that one initial effects active melanoma cells alteration cellular energy metabolism specifically increase aerobic glycolysis. This was found to be at least part due Akt lactate...
Identification of individuals who are at risk suffering from acute coronary syndromes (ACS) may allow to introduce preventative measures. We aimed identify ACS-related urinary peptides, that combined as a pattern can be used prognostic biomarker. Proteomic data 252 enrolled in four prospective studies Australia, Europe and North America were analyzed. 126 these had suffered ACS within period up 5 years post urine sampling (cases). analysis 84 cases matched controls resulted the discovery 75...
Background Detection of preclinical cardiac dysfunction and prognosis left ventricular heart failure ( HF ) would allow targeted intervention, appears to be the most promising approach in its management. Novel biomarker panels may support this provide new insights into pathophysiology. Methods Results A retrospective comparison urinary proteomic profiles generated by mass spectrometric analysis from 49 patients, 36 patients who progressed within 2.6±1.6 years, 192 sex‐ age‐matched controls...
// Esther Nkuipou-Kenfack 1,2 , Akshay Bhat 1,3 Julie Klein 4,5 Vera Jankowski 6 William Mullen 7 Antonia Vlahou 8,9 Mohammed Dakna 1 Thomas Koeck Joost P. Schanstra 5,6 Petra Zürbig Karl L. Rudolph 10 Björn Schumacher 11 Andreas Pich 2 and Harald Mischak 1,7 Mosaiques Diagnostics GmbH, Hannover, Germany Hannover Medical School, Core Facility Proteomics, 3 Charité-Universitätsmedizin Berlin, Med. Klinik IV, 4 Institut National de la Santé et Recherche Médicale (INSERM), of Cardiovascular...
Current knowledge on the pathogenesis of diastolic heart failure predominantly rests case-control studies involving symptomatic patients with preserved ejection fraction and relying invasive diagnostic procedures including endomyocardial biopsy. Our objective was to gain insight in serum urinary biomarkers reflecting collagen turnover associated asymptomatic LV dysfunction. We randomly recruited 782 Flemish (51.3% women; 50.5 years). assessed function from early late peak velocities...