A5089711245- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- SARS-CoV-2 detection and testing
- Legionella and Acanthamoeba research
- Bacterial Identification and Susceptibility Testing
- Antibiotic Resistance in Bacteria
- COVID-19 and healthcare impacts
- Antibiotics Pharmacokinetics and Efficacy
- Clostridium difficile and Clostridium perfringens research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Neonatal Health and Biochemistry
- Multiple Myeloma Research and Treatments
- Infective Endocarditis Diagnosis and Management
- Viral gastroenteritis research and epidemiology
- Methemoglobinemia and Tumor Lysis Syndrome
- Biosensors and Analytical Detection
- Cardiac, Anesthesia and Surgical Outcomes
- Infections and bacterial resistance
- Bacterial biofilms and quorum sensing
- COVID-19 epidemiological studies
- Hemoglobinopathies and Related Disorders
- Antifungal resistance and susceptibility
- Antibiotic Use and Resistance
- Hematopoietic Stem Cell Transplantation
- COVID-19 and Mental Health
Mayo Clinic Hospital
2024-2025
University of Washington
2012-2024
University of Washington Medical Center
2017-2021
Northwest Hospital and Medical Center
2021
Emory University Hospital
2020
Wrightington Hospital
2020
Integral Molecular (United States)
2019
University of Michigan
2010-2014
WellStar Kennestone Hospital
2013
The Ohio State University
2012
Coronavirus disease 2019 (COVID-19), the novel respiratory illness caused by severe acute syndrome coronavirus 2 (SARS-CoV-2), is associated with morbidity and mortality. The rollout of diagnostic testing in United States was slow, leading to numerous cases that were not tested for SARS-CoV-2 February March 2020 necessitating use serological determine past infections. Here, we evaluated Abbott IgG test detection anti-SARS-CoV-2 antibodies 3 distinct patient populations. We 1,020 serum...
Nonselected and natural populations of Escherichia coli from 12 animal sources humans were examined for the presence types 14 tetracycline resistance determinants. Of 1,263 unique E. isolates humans, pigs, chickens, turkeys, sheep, cows, goats, cats, dogs, horses, geese, ducks, deer, 31% highly resistant to tetracycline. More than 78, 47, 41% turkeys or tetracycline, respectively. Tetracycline MICs 61, 29, 29% pig, respectively, >/=233 micro g/ml. Muliplex PCR analyses indicated that 97%...
Legionella pneumophila is an intracellular pathogen responsible for Legionnaires' disease. This bacterium uses the Dot/Icm type IV secretion system to inject a large number of bacterial proteins into host cells facilitate biogenesis phagosome permissive its growth. Like many highly adapted intravacuolar pathogens, L. able maintain neutral pH in lumen phagosome, particularly early phase infection. However, all cases, molecular mechanisms underlying this observation remain unknown. In report,...
Molecular syndromic diagnostic panels can enhance pathogen identification in the approximately 2-4 billion episodes of acute gastroenteritis that occur annually worldwide. However, clinical utility these has not been established.We conducted a prospective, multi-center study to investigate impact BioFire FilmArray Gastrointestinal polymerase chain reaction panel on diagnosis and decision-making, compared acuity patients with positive results obtained exclusively those detected by...
Immunopathology may play a significant role in the pathogenesis of Coronavirus-Induced Disease-19 (COVID-19). Immune-mediated tissue damage could result from development rapid autoimmune responses, characterized by production self-reactive autoantibodies. In this study, we tested specimens acutely ill patients hospitalized with COVID-19 for autoantibodies against nuclear, vasculitis-associated, and phospholipid antigens. Detectable were present 30% our cohort, majority reactive demonstrating...
BACKGROUND. SARS-CoV-2–specific antibodies may protect from reinfection and disease, providing rationale for administration of plasma containing SARS-CoV-2–neutralizing (nAbs) as a treatment COVID-19. Clinical factors laboratory assays to streamline donor selection, the durability nAb responses, are incompletely understood.
Abstract Background Healthcare workers (HCWs) who serve on the front lines of coronavirus disease 2019 (COVID-19) pandemic have been at increased risk for infection due to severe acute respiratory syndrome 2 (SARS-CoV-2) in some settings. Healthcare-acquired has reported similar epidemics, but there are limited data prevalence COVID-19 among HCWs and their associated clinical outcomes United States. Methods We established high-throughput employee testing centers Seattle, Washington, with...
With the availability of widespread SARS-CoV-2 vaccination, high-throughput quantitative antispike serological testing will likely become increasingly important. Here, we investigated performance characteristics recently FDA-authorized semiquantitative AdviseDx IgG II assay compared to antinucleocapsid Abbott Architect IgG, Roche Elecsys anti-SARS-CoV-2-S, EuroImmun anti-SARS-CoV-2 enzyme-linked immunosorbent (ELISA), and GenScript surrogate virus neutralization assays examined humoral...
Background Coronavirus disease-19 (COVID19), the novel respiratory illness caused by severe acute syndrome coronavirus 2 (SARS-CoV-2), is associated with morbidity and mortality. The rollout of diagnostic testing in United States was slow, leading to numerous cases that were not tested for SARS-CoV-2 February March 2020, necessitating use serological determine past infections. Methods We evaluated Abbott IgG test detection anti-SARS-CoV-2 antibodies 3 distinct patient populations. Results...
We found low prevalence of SARS-CoV-2 (2.7% [5/188]) among pregnant and postpartum patients with universal testing. Prevalence symptomatic was similar under initial targeted screening (22.2% [4/18]) approaches (19.1% [8/42]). Among 170 asymptomatic patients, 2 were positive or inconclusive, respectively; repeat testing at 24 hours negative.
Genomic DNA sequence analysis of the uropathogenic Escherichia coli strain CFT073 revealed that besides fimB and fimE recombinase genes control type 1 pilus fim phase switch, there are three additional fimB- fimE-like genes: ipuA, ipuB, ipbA. Alignment predicted amino acid sequences showed five recombinases range in similarity from 63 to 70%. An epidemiological survey indicates ipuA ipuB present linked next dsdCXA locus 24 67 E. strains but found only 15 normal human fecal isolates. The ipbA...
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) viral load on admission was associated with a significantly increased 30-day mortality (odds ratio [OR], 4.20; 95% CI, 1.62-10.86), and anti-SARS-CoV-2 nucleocapisid IgG seropositivity trended toward reduced (OR, 0.43; 0.15-1.26). Reporting of quantitative SARS-CoV-2 serologic assays may offer prognostic clinical information.
Broad-range fungal PCR is a powerful tool for identifying pathogens directly from patient specimens; however, reported estimates of clinical utility vary and costs discourage universal testing. We investigated the diagnostic broad-range by examining 9 years results sinonasal specimens, hypothesizing that this anatomic location would identify immunocompromised patients at high risk invasive disease. retrospectively identified 644 PCRs 1,446 cultures sinus sites. To determine relative...
Introduction Screening for G6PD deficiency can inform disease management including malaria. Treatment with the antimalarial drugs primaquine and tafenoquine be guided by point-of-care testing deficiency. Methods findings Data from similar clinical studies evaluating performance of STANDARD Test (SD Biosensor, South Korea) conducted in Bangladesh, Brazil, Ethiopia, India, Thailand, United Kingdom, States were pooled. was assessed a retrospective analysis on capillary venous specimens. All...
Abstract Metagenomic sequencing of plasma has been advertised by Karius, Inc. as a way to diagnose variety infectious syndromes. Due the lack robust evidence clinical utility, our laboratory began actively stewarding Karius testing. Microbiology Directors recommended cancelation orders when certain criteria were identified. We set out review test requests in 52-month period stewardship, during which we cancellation on 21 57 (37%). Of tests sent samples with negative conventional testing,...
Campylobacter species are among the most common causes of enteric bacterial infections worldwide. Men who have sex with men (MSM) at increased risk for sexually transmitted infections, including globally distributed strains multidrug-resistant Shigella species.This was a retrospective study MSM-associated in Seattle, Washington and Montréal, Québec phenotypic antimicrobial resistance profiles whole genome sequencing (WGS).We report isolation 2 clonal lineages coli from MSM Seattle Montréal....
Cefiderocol is a siderophore cephalosporin active against MDR Gram-negatives including Stenotrophomonas maltophilia. resistance remains uncommon and incompletely understood. We selected for cefiderocol-resistant S. maltophilia in vitro characterized the genetic mechanisms potential cross-resistance to other antimicrobials.We cefiderocol three clinical strains of by serial passage escalating concentrations cefiderocol. Emergent isolates were subjected repeat susceptibility testing panel...
Staphylococcus aureus generates biofilms during many chronic human infections, which contributes to its growth and persistence in the host. Multiple genes pathways necessary for S. biofilm production have been identified, but knowledge is incomplete, little known about spontaneous mutations that increase formation as infection progresses. Here, we performed vitro selection of four laboratory strains (ATCC 29213, JE2, N315, Newman) identify associated with enhanced production. Biofilm...
Abstract Objectives To assess the concordance and performance characteristics of Helicobacter pylori laboratory tests compared with histopathology to propose algorithms for diagnosis H that minimize diagnostic error. Methods diagnostics were reviewed from a 12-year period within health system (2,560 cases). Analyses performed adjust based on treatment consensus histopathologic diagnoses among pathologists. Markers access care, including test cancellation frequency turnaround time, assessed....