A5064000145- Photonic Crystals and Applications
- SARS-CoV-2 and COVID-19 Research
- SARS-CoV-2 detection and testing
- Optical Coatings and Gratings
- Advanced Biosensing Techniques and Applications
- COVID-19 Clinical Research Studies
- Clinical Laboratory Practices and Quality Control
- Photonic and Optical Devices
- COVID-19 epidemiological studies
- Biosensors and Analytical Detection
- Plasmonic and Surface Plasmon Research
- Microfluidic and Capillary Electrophoresis Applications
- Statistical Methods in Clinical Trials
- Electronic Health Records Systems
- Pharmacogenetics and Drug Metabolism
- Near-Field Optical Microscopy
- Health Systems, Economic Evaluations, Quality of Life
- Blood transfusion and management
- Potassium and Related Disorders
- Meta-analysis and systematic reviews
- COVID-19 and healthcare impacts
- Nanofabrication and Lithography Techniques
- Respiratory viral infections research
- Bacillus and Francisella bacterial research
- COVID-19 and Mental Health
University of Washington
2015-2024
Michigan Medicine
2023
University of Michigan
2023
Seattle University
2017-2022
University of Washington Medical Center
2020
Seattle Reproductive Medicine
2018
University of Illinois Urbana-Champaign
2005-2011
Urbana University
2009
Northwestern University
2006
National Institute for Occupational Safety and Health
1995
Coronavirus disease 2019 (COVID-19), the novel respiratory illness caused by severe acute syndrome coronavirus 2 (SARS-CoV-2), is associated with morbidity and mortality. The rollout of diagnostic testing in United States was slow, leading to numerous cases that were not tested for SARS-CoV-2 February March 2020 necessitating use serological determine past infections. Here, we evaluated Abbott IgG test detection anti-SARS-CoV-2 antibodies 3 distinct patient populations. We 1,020 serum...
Abstract Background UW Medicine was one of the first health systems to encounter and treat COVID-19 patients in United States, starting late February 2020. Objective Here we describe rapid rollout capabilities by Information Technology Services (ITS) support our clinical response pandemic provide recommendations for urgently consider, as they plan their own this potentially other future pandemics. Methods Our include establishing a hospital incident command structure that includes tight...
Abstract The COVID-19 epidemic of 2019–20 is due to the novel coronavirus SARS-CoV-2. Following first case description in December, 2019 this virus has infected over 10 million individuals and resulted at least 500,000 deaths world-wide. undergoing rapid mutation, with two major clades sequence variants emerging. This study sought determine whether SARS-CoV-2 are associated differing outcomes among patients a single medical system. Whole genome RNA was obtained from isolates collected...
The coronavirus disease 2019 (COVID-19) pandemic is dominated by variant viruses; the resulting impact on severity remains unclear. Using a retrospective cohort study, we assessed hospitalization risk following infection with 7 severe acute respiratory syndrome 2 (SARS-CoV-2) variants.Our study includes individuals positive SARS-CoV-2 reverse transcription polymerase chain reaction (RT-PCR) in Washington Disease Reporting System available viral genome data, from 1 December 2020 to 14 January...
Efficient recovery of light emitted by fluorescent molecules employing photonic structures can result in high signal-to-noise ratio detection for biological applications including DNA microarrays, fluorescence microscopy and single molecule detection. By a model system comprised colloidal quantum dots, we consider the physical basis extraction effect as provided crystals. Devices with different lattice symmetry are fabricated ensuring spectral spatial coupling dot emission leaky eigenmodes...
Photonic crystal surfaces are demonstrated as a means for enhancing the detection sensitivity and resolution assays that use fluorescent tag to quantify concentration of an analyte protein molecule in liquid test sample. Computer modeling spatial distribution resonantly coupled electromagnetic fields on photonic surface used estimate magnitude enhancement factor compared performing same assay plain glass surface, structure is fabricated tested experimentally verify performance using sandwich...
Background Coronavirus disease-19 (COVID19), the novel respiratory illness caused by severe acute syndrome coronavirus 2 (SARS-CoV-2), is associated with morbidity and mortality. The rollout of diagnostic testing in United States was slow, leading to numerous cases that were not tested for SARS-CoV-2 February March 2020, necessitating use serological determine past infections. Methods We evaluated Abbott IgG test detection anti-SARS-CoV-2 antibodies 3 distinct patient populations. Results...
Real-time epidemiological tracking of variants concern (VOCs) can help limit the spread more contagious forms severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), such as those containing N501Y mutation. Typically, genetic sequencing is required to be able track VOCs in real-time. However, take time and may not accessible all laboratories. Genotyping by RT-ddPCR offers an alternative rapidly detect through discrimination specific alleles N501Y, which associated with increased...
SARS-CoV-2 transmission is largely driven by heterogeneous dynamics at a local scale, leaving health departments to design interventions with limited information. We analyzed genomes sampled between February 2020 and March 2022 jointly epidemiological cell phone mobility data investigate fine scale spatiotemporal in King County, Washington, diverse, metropolitan US county. applied an approximate structured coalescent approach model within North County South alongside the rate of outside...
We report on the design and demonstration of an optical imaging system capable exciting surface-bound fluorophores within resonant evanescent electric field a photonic crystal surface gathering fluorescence emission that is directed toward objective by crystal. The also has ability to quantify shifts in local resonance angle induced adsorption biomolecules for label-free biomolecular imaging. With these two capabilities combined single detection system, we demonstrate images self-registered...
Unrecognized pseudohyperkalemia (PHK), defined as an artificial increase in measured potassium concentration, due to thrombocytosis and leukocytosis can lead inappropriate patient treatment. Understanding the laboratory characteristics that risk of PHK is key preventing diagnostic errors. Serum/plasma results collected at 2 laboratories over 4 years were selected based on blood cell counts within 24 h whole concentrations determined serum/plasma sample. Differences between serum or plasma...
Many point-of-care laboratory tests are manually entered into the electronic health record by ambulatory clinic staff, but rate of manual transcription error for this testing is poorly characterized. Using a dataset arising from duplicated workflow that created set paired interfaced and glucose measurements, we found 260 6930 (3.7%) entries were discrepant their result. Thirty-seven (14.2%) errors more than 20% included potentially dangerous mistranscriptions. An additional 37 due to...
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) viral load on admission was associated with a significantly increased 30-day mortality (odds ratio [OR], 4.20; 95% CI, 1.62-10.86), and anti-SARS-CoV-2 nucleocapisid IgG seropositivity trended toward reduced (OR, 0.43; 0.15-1.26). Reporting of quantitative SARS-CoV-2 serologic assays may offer prognostic clinical information.
Abstract Background The COVID-19 pandemic is dominated by variant viruses; the resulting impact on disease severity remains unclear. Using a retrospective cohort study, we assessed hospitalization risk following infection with seven SARS-CoV-2 variants. Methods Our study includes individuals positive RT-PCR in Washington Disease Reporting System available viral genome data, from December 1, 2020 to January 14, 2022. analysis was restricted cases specimens collected through sentinel...
Broad-range fungal PCR is a powerful tool for identifying pathogens directly from patient specimens; however, reported estimates of clinical utility vary and costs discourage universal testing. We investigated the diagnostic broad-range by examining 9 years results sinonasal specimens, hypothesizing that this anatomic location would identify immunocompromised patients at high risk invasive disease. retrospectively identified 644 PCRs 1,446 cultures sinus sites. To determine relative...
A 2D photonic crystal surface with a different period in each lateral direction is demonstrated to detect biomolecules using two distinct sensing modalities. The mechanisms both rely on the generation of resonant reflection peak at one specific wavelengths, depending polarization light that incident crystal. One results visible spectrum coincide excitation wavelength fluorophore, while orthogonal an infrared which used for label-free detection adsorbed biomolecules. resonance fluorescence...
The emission intensity of is fluorescent dye molecules increased by over two orders magnitude through the combined effects enhanced near fields produced a 1D photonic crystal slab and surface area provided nanorod-structured TiO2 film (see image).
DNA microarrays are used to profile changes in gene expression between samples a high-throughput manner, but measurements of genes with low levels can be problematic standard microarray substrates. In this work, we expand the detection capabilities experiment using photonic crystal (PC) surface that enhances fluorescence observed from spots. This PC is inexpensively and uniformly fabricated nanoreplica molding technique, very little variation its optical properties within- between-devices....
Abstract Improving the performance and lowering analyte detection limits of optical electronic biosensors is essential for advancing wide ranging applications in diagnostics drug discovery. Most sensing methods require direct linkage a recognition element sensor, which commonly accomplished through an organic monolayer interface. Alkoxyorganosilanes are typically used to prepare sensor surfaces on dielectric oxides. However, many silanes lead roughened or thick interfaces that degrade device...
Surface-bound fluorescence assays such as microarrays have emerged a prominent technology in current life sciences research and are currently performed on optically passive substrates glass microscope slides. We present an alternative approach using photonic crystal exhibiting resonant reflections. In this work, we design fabricate with TM-polarized resonance at the cyanine-5 excitation wavelength TE-polarized spectrally overlapping fluorophore's emission spectrum. The former increases of...
Real-time polymerase chain reaction (RT-PCR) detection of severe acute respiratory syndrome coronavirus (SARS-CoV-2) is required for diagnosis disease 2019 (COVID-19). Sensitivity RT-PCR nasopharyngeal (NP) testing presumed to be high, but there no gold standard against which this has been determined. The objective was determine whether lower tract infection (LRTI), detected in bronchoalveolar lavage fluid (BALF), occurs the absence upper with clinical both specimen types. Between March 26,...
We experimentally verify the near-field nature of fluorescence enhancement afforded by large area planar photonic crystal resonators. This is done mapping factor for a fluorophore as function its distance above device surface and comparing results to numerical calculations. Experiments show that decay exponential from surface, excellent agreement with numerically calculated length obtained. In contrast approaches involving plasmons on metal surfaces, we find maximum obtained when closest due...