A5010678114- Clinical Laboratory Practices and Quality Control
- Meta-analysis and systematic reviews
- Advanced biosensing and bioanalysis techniques
- Health Systems, Economic Evaluations, Quality of Life
- Opioid Use Disorder Treatment
- Advanced Fluorescence Microscopy Techniques
- Molecular Biology Techniques and Applications
- Advanced Biosensing Techniques and Applications
- Radiomics and Machine Learning in Medical Imaging
- Monoclonal and Polyclonal Antibodies Research
- Healthcare cost, quality, practices
- Statistical Methods in Clinical Trials
- Advanced Proteomics Techniques and Applications
- Cannabis and Cannabinoid Research
- Healthcare Policy and Management
- Photoreceptor and optogenetics research
- Renal function and acid-base balance
- Pharmacogenetics and Drug Metabolism
- Forensic Toxicology and Drug Analysis
- Sarcoma Diagnosis and Treatment
- Biosensors and Analytical Detection
- bioluminescence and chemiluminescence research
- HER2/EGFR in Cancer Research
- Potassium and Related Disorders
- Colorectal Cancer Screening and Detection
University of Washington
2016-2025
University of Washington Medical Center
2011-2022
Walter de Gruyter (Germany)
2022
Azienda Ospedaliera di Padova
2022
University of Padua
2022
National University Hospital
2018
Seattle University
2008-2018
Neurological Surgery
2017
Stanford University
2017
Illinois College
2017
Civil infrastructure will be essential to face the interlinked existential threats of climate change and rising resource demands while ensuring a livable Anthropocene for all. However, conventional planning largely neglects ...
Yellow mutants of the green fluorescent protein (YFP) are crucial constituents genetically encoded indicators signal transduction and fusions to monitor protein-protein interactions. However, previous YFPs show excessive pH sensitivity, chloride interference, poor photostability, or expression at 37 °C. Protein evolution in <i>Escherichia coli</i> has produced a new YFP named Citrine, which mutation Q69M confers much lower p<i>K</i> <sub>a</sub> (5.7) than for YFPs, indifference chloride,...
Many areas of biology and biotechnology have been revolutionized by the ability to label proteins genetically fusion Aequorea green fluorescent protein (GFP). In previous fusions, GFP has treated as an indivisible entity, usually appended amino or carboxyl terminus host protein, occasionally inserted within sequence. The tightly interwoven, three-dimensional structure intricate posttranslational self-modification required for chromophore formation would suggest that major rearrangements...
DsRed is a recently cloned 28-kDa fluorescent protein responsible for the red coloration around oral disk of coral Discosoma genus. has attracted tremendous interest as potential expression tracer and fusion partner that would be complementary to homologous green from Aequorea , but very little known biochemistry DsRed. We now show much higher extinction coefficient quantum yield than previously reported, plus excellent resistance pH extremes photobleaching. In addition, its 583-nm emission...
DsRed, a brilliantly red fluorescent protein, was recently cloned from Discosoma coral by homology to the green protein (GFP) jellyfish Aequorea . A core question in biochemistry of DsRed is mechanism which GFP-like 475-nm excitation and 500-nm emission maxima immature are red-shifted 558-nm 583-nm mature DsRed. After digestion with lysyl endopeptidase, high-resolution mass spectra purified chromophore-bearing peptide reveal that some molecules have lost 2 Da relative analogously prepared...
Biomarkers are urgently needed for the diagnosis and monitoring of disease progression in Parkinson’s disease. Both DJ-1 α-synuclein, two proteins critically involved pathogenesis, have been tested as biomarkers several recent studies with inconsistent results. These largely due to variation protein species detected by different antibodies, limited numbers patients some studies, or inadequate control important variables. In this study, nature α-synuclein human cerebrospinal fluid was studied...
After its emergence in Wuhan, China, late November or early December 2019, the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus rapidly spread globally. Genome sequencing of SARS-CoV-2 allows reconstruction transmission history, although this is contingent on sampling. We analyzed 453 genomes collected between 20 February and 15 March 2020 from infected patients Washington state United States. find that most infections sampled during time derive a single introduction...
Gene expression of intrinsically fluorescent proteins in biological systems offers new noninvasive windows into cellular function, but optimization these probes relies on understanding their molecular spectroscopy, dynamics, and structure. Here, the photophysics red protein (dsRed) from discosoma ( coral ), providing desired longer emission/absorption wavelengths, an improved yellow mutant (Citrine) (S65G/V68L/Q69 M/S72A/T203Y) for significant comparison, are characterized by using...
An important precondition for the successful development of diagnostic assays cerebrospinal fluid (CSF) biomarkers age-related neurodegenerative diseases is an understanding dynamic nature CSF proteome during normal aging process. In this study, a novel proteomic technology was used to quantify hundreds proteins simultaneously in from 90 cognitively adults 21 85 years age. SomaLogic's highly multiplexed platform can measure more than 800 small volumes biological fluids using slow off-rate...
Lung cancer remains the most common cause of cancer-related mortality. We applied a highly multiplexed proteomic technology (SOMAscan) to compare protein expression signatures non small-cell lung (NSCLC) tissues with healthy adjacent and distant from surgical resections. In this first report SOMAscan tissues, we highlight 36 proteins that exhibit largest differences between matched tumor non-tumor tissues. The concentrations twenty increased sixteen decreased in tissue, thirteen which are...
Abstract Following its emergence in Wuhan, China, late November or early December 2019, the SARS-CoV-2 virus has rapidly spread throughout world. Genome sequencing of strains allows for reconstruction transmission history connecting these infections. Here, we analyze 346 genomes from samples collected between 20 February and 15 March 2020 infected patients Washington State, USA. We found that large majority infections sampled during this time frame appeared to have derived a single...
Abstract To ensure that clinical laboratories produce results are both accurate and of utility it is essential only samples adequate quality analysed. Although various studies databases assessing the stability analytes in different settings do exist, guidance on how to perform report lacking. This often not information, thus compromising transferability data. The aim this manuscript describe C hecklist for R eporting S tability tudies (CRESS) against which future should be reported...
Mutations in the catalytic subunit of protein kinase A (PKAc) drive stress hormone disorder adrenal Cushing's syndrome. We define mechanisms action for PKAc-L205R and W196R variants. Proximity proteomic techniques demonstrate that both mutants are excluded from kinase-anchoring (AKAP)-signaling islands, whereas live-cell photoactivation microscopy reveals these indiscriminately diffuse throughout cell. Only cAMP analog drugs displace native PKAc AKAPs enhance cortisol release. Rescue...
Abstract Objectives Knowledge of the stability analytes in clinical specimens is a prerequisite for proper transport and preservation samples to avoid laboratory errors. The new version ISO 15189:2022 European directive 2017/746 increase requirements on this topic manufacturers laboratories. Within project generate database Federation Clinical Chemistry Laboratory Medicine (EFLM) Working Group Preanalytical Phase (WG-PRE), need standardise improve quality published studies has been detected,...
Camgaroos are yellow fluorescent protein derivatives that hold promise as transgenically encoded calcium sensors in behaving animals. We expressed two versions of camgaroo Drosophila mushroom bodies using the galactosidase-4 (GAL4) system. Potassium depolarization brains expressing reporters produces a robust increase fluorescence is blocked by removing extracellular or antagonists voltage-dependent channels. The not attributable to cytoplasmic alkalization; induces slight acidification...