A5035756331- Infection Control in Healthcare
- Infection Control and Ventilation
- Medical Device Sterilization and Disinfection
- Food Safety and Hygiene
- Dental Research and COVID-19
- Antimicrobial agents and applications
- Viral gastroenteritis research and epidemiology
- Bacterial Genetics and Biotechnology
- Intramuscular injections and effects
- Clostridium difficile and Clostridium perfringens research
- Viral Infections and Immunology Research
- Antibiotic Resistance in Bacteria
- Antibiotic Use and Resistance
- Patient Safety and Medication Errors
- Safe Handling of Antineoplastic Drugs
- Pressure Ulcer Prevention and Management
- Bacillus and Francisella bacterial research
- Antimicrobial Resistance in Staphylococcus
- Enzyme Structure and Function
- Genomics and Phylogenetic Studies
- Surgical site infection prevention
- Microbial Natural Products and Biosynthesis
- Pregnancy-related medical research
- Bacterial biofilms and quorum sensing
- Pharmacy and Medical Practices
Gojo (United States)
2012-2023
Engineering Associates (United States)
2017
Northeast Ohio Medical University
2012-2015
Biology of Infection
2015
University of Akron
2012-2014
Gojo (France)
2010-2014
Children's Hospital Association
2013
Office of the Chief Scientist
2011
Microbiology Society
2011
Procter & Gamble (United States)
2003
Alcohol-based hand sanitizers are being recommended as an infection prevention measure for COVID-19. Recently published data indicates that ethanol effectively inactivates the SARS-CoV-2 virus, but there is a lack of formulated sanitizer products currently used in U.S. healthcare and general settings. This study demonstrates commercially available foam gel alcohol-based effective inactivating suspension.
Norovirus is the leading cause of food-related illness in United States, and contamination ready-to-eat items by food handlers poses a high risk for disease. This study reports vitro (suspension test) vivo (fingerpad protocol) assessments new ethanol-based hand sanitizer containing synergistic blend polyquaternium polymer organic acid, which active against viruses public health importance, including norovirus. When tested suspension, test product reduced infectivity nonenveloped human...
This study determined whether surrogate organisms can predict activity against Clostridium difficile spores and compared the efficacy of hand hygiene preparations C. difficile. Our data suggest that were not predictive spore removal. Four significantly more effective than tap water at removing
ABSTRACT Bulk-soap-refillable dispensers are prone to extrinsic bacterial contamination, and recent studies demonstrated that approximately one in four public restrooms contaminated. The purpose of this study was quantify hand contamination transfer after use contaminated soap under controlled laboratory in-use conditions a community setting. Under using liquid experimentally with 7.51 log 10 CFU/ml Serratia marcescens , an average 5.28 CFU remained on each washing, 2.23 transferred agar...
Alcohol-based hand rubs (ABHR) range in alcohol concentration from 60-95% and are available a variety of delivery formats, such as rinses, gels, foams. Recent studies suggest that some ABHR foams dry too slowly, thereby encouraging the use inadequate volumes. This study investigates influence product volume, format, on dry-time antimicrobial efficacy foams, gels rinses. dry-times were measured using volunteers to determine influences concentration. efficacies evaluated according European...
ABSTRACT Providencia stuartii contains a chromosomal 2′- N -acetyltransferase [AAC(2′)-Ia] involved in the O acetylation of peptidoglycan. The AAC(2′)-Ia enzyme is also capable acetylating and inactivating certain aminoglycosides confers high-level resistance to these antibiotics when overexpressed. We report identification locus P. , designated aarF that required for expression AAC(2′)-Ia. Northern (RNA) analysis demonstrated aac(2′)-Ia mRNA levels were dramatically decreased strain...
The literature on hand washing, while extensive, often contains conflicting data, and key variables are only superficially studied or not at all. Some washing recommendations made without scientific support, agreement between is limited. influence of such as soap volume, lather time, water temperature, product formulation efficacy was investigated in the present study. Baseline conditions were 1 mL a bland (nonantimicrobial) soap, 5-s 38°C (100°F) temperature. A nonpathogenic strain...
Abstract Background The World Health Organization has called for the development of improved methodologies to evaluate alcohol-based handrub (ABHR) efficacy, including evaluation at “short application times and volumes that reflect actual use in healthcare facilities”. objective this study was investigate variables influencing ABHR under test conditions reflective clinical use. Methods product (60% V /V 2-propanol) evaluated according a modified EN 1500 methodology, where 1 mL, 2 3 mL were...
The aarP gene has been identified in a search for activators of the 2-N-acetyltransferase [encoded by aac(2')-Ia] Providencia stuartii. Introduction into P. stuartii on multicopy plasmid resulted 9.9-fold increase accumulation beta-galactosidase from an aac(2')-lacZ fusion. Northern (RNA) blot analysis demonstrated that this increased aac(2')-Ia expression occurred at level mRNA accumulation. deduced AarP protein was 15,898 Da size and exhibited significant homology to number transcriptional...
Of 82 patients with methicillin-resistant Staphylococcus aureus (MRSA) colonization, 67 (82%) had positive hand cultures for MRSA. A single application of alcohol gel (2 mL) consistently reduced the burden MRSA on hands. However, incomplete removal was common, particularly in those a high baseline level recovery. Infect Control Hosp Epidemiol 2014;00(0):1–3
The cervix undergoes profound changes in size and consistency during pregnancy which are designed to facilitate a normal delivery. These under the control of number hormonal factors. Experimental clinical studies suggest that relaxin, protein hormone, plays role promoting cervical softening. One possible target site for effects relaxin on is stroma. In reported here, cultures human stromal cells were used determine what direct has cells. Relaxin at concentrations ranging from 0.10-100 ng/mL...
In vivo efficacies of 2 alcohol-based hand rub (ABHR) products (gel and foam) were evaluated at a volume 1.1 mL. Both met US Food Drug Administration log(10) reduction requirements after single application 10 consecutive applications. This is the first study to identify ABHR formulations capable meeting efficacy with single-dispenser actuation.
A novel method has been developed for the evaluation of alcohol-based hand rubs (ABHR) that employs a contamination procedure more closely simulates in-use conditions ABHR. Hands human subjects were contaminated with 0.2 ml concentrated suspension Serratia marcescens (ATCC 14756) to achieve baseline between 8 and 9 log₁₀ CFU/hand while allowing product be applied dry hands minimal soil load. Evaluation 1.5 an ABHR gel containing 62% ethanol produced reductions 2.66 ± 0.96, 2.40 0.50, 2.41...
In a search for genes involved in regulation of the 2′‐ N ‐acetyltransferase Providencia stuartii , mini‐Tn 5 Cm insertion has been isolated locus designated aarD . The aarD1 ::mini‐Tn mutation resulted 4.7‐fold increase levels β‐galactosidase accumulation from an aac(2′)–lacZ transcriptional fusion and 32‐fold gentamicin resistance P. wild‐type was cloned on 5.0 kb Cla I fragment complemented mutation. Nucleotide sequence analysis this identified two large open reading frames whose deduced...