Abstract Resistance of benzimidazole fungicides is related to the point mutation β-tubulin gene in Sclerotinia sclerotiorum . The at codon 198 (GAG → GCG, E198A) occurs more than 90% field resistant populations China. Traditional detection methods benzimidazole-resistant mutants S. are time-consuming, tedious and inefficient. To establish a suitable rapid , an efficient simple method with high specificity was developed based on loop-mediated isothermal amplification (LAMP). Eight sets LAMP...

Summary Salt and drought impair plant osmotic homeostasis greatly limit growth development. Plants decrease stomatal aperture to reduce water loss maintain homeostasis, leading improved stress tolerance. Herein, we identified the C 2 H transcription factor gene OSMOTIC STRESS INDUCED 1 ( OSIC1 ) from Populus alba var. pyramidalis be induced by salt, drought, polyethylene glycol 6000 (PEG6000) abscisic acid (ABA). Overexpression of conferred transgenic poplar more tolerance high salinity,...

Planting aluminum-tolerant legume green manure is a cost-effective and sustainable method to increase soil fertility as well decrease Al toxicity in acidic soils. By analyzing the relative root elongation of seven species, common vetch (Vicia sativa L.) was identified an Al-resistant species. Furthermore, cultivars 418 (cv. Sujian No.3) 426 Lanjian were -sensitive cultivars, respectively, among 12 germplasms. The growth less inhibited by both germination stage seedling than that 426. Under...

WRKY transcription factors (TFs) play a vital part in coping with different stresses. In this study, DgWRKY2 was isolated from Dendranthema grandiflorum. The gene encodes 325 amino acid protein, belonging to the group II family, and contains one typical domain (WRKYGQK) zinc finger motif (C-X4-5-C-X22-23-H-X1-H). Overexpression of chrysanthemum enhanced tolerance high-salt stress compared wild type (WT). addition, activities antioxidant enzymes (superoxide dismutase (SOD), peroxidase (POD),...

Abstract Background Low-temperature severely affects the growth and development of chrysanthemum which is one kind ornamental plant well-known widely used in world. Lysine crotonylation a recently identified post-translational modification (PTM) with multiple cellular functions. However, lysine under low-temperature stress has not been studied. Results Proteome-wide at was analyzed using TMT (Tandem Mass Tag) labeling, sensitive immuno-precipitation, high-resolution LC-MS/MS. The results...

Drought is one of the main factors affecting sweet cherry yields, and rootstocks can provide a range tree vigor levels to better match cherries with characteristics soil. To investigate molecular events water deficiency, we performed transcriptomic metabolomic analyses Prunus mahaleb CDR-1 (drought-tolerant rootstock (DT)) P. cerasus × canescens Gisela 5 (drought-susceptible (DS)), respectively. The results revealed 253 common drought-responsive genes in leaves roots DT 17 DS; 59 upregulated...

A novel primase inhibitor, Sch 642305 (1), was isolated from the fermentation broth of fungal culture Penicillium verrucosum. The structure 1 elucidated on basis MS and NMR spectroscopic data as a new unusual bicyclic 10-membered macrolide. absolute configuration asymmetric centers determined by X-ray crystallographic analysis p-bromobenzoate derivative (3). Compound exhibited inhibitory activity against bacterial DNA enzyme with an EC(50) 70 microM.

Lily (Lilium spp.) is an important commercial flower crop, but its market popularity and applications are adversely affected by severe pollen pollution. Many studies have examined development in model plants, few been conducted on crops such as lily. GAMYBs a class of R2R3-MYB transcription factors play roles plant biotic resistance; their functions vary different pathways, many them involved anther development. However, function regulatory role lily remain unclear. Here, the GAMYB homolog...

Salt response has long been considered a polygenic-controlled character in plants. Under salt stress conditions, plants respond by activating great amount of proteins and enzymes. To develop better understanding the molecular mechanism screen responsive genes chrysanthemum under stress, we performed RNA sequencing (RNA-seq) on both salt-processed seedling roots control group, gathered six cDNA databases eventually. Moreover, to overcome Illumina HiSeq technology's limitation sufficient...

Drought is an important abiotic factor that threatens the growth and development of plants. Verbena bonariensis a widely used landscape plant with very high ornamental value. We found has drought tolerance in production practice, so order to delve into its mechanism resistance screen out drought-resistance genes, we RNA-Seq platform perform de novo transcriptome assembly analyze transcription response stress. By high-throughput sequencing Illumina Hiseq Xten, total 44.59 Gb clean data was...

Concerns about resistance development to conventional insecticides in diamondback moth (DBM) Plutella xylostella (L.), the most destructive pest of Brassica vegetables, have stimulated interest alternative management strategies. The toxicity Bacillus thuringiensis subsp. aizawai (Bt GO33A) combined with chlorantraniliprole (Chl) has not been documented. Here, we examined single and Bt assess levels four DBM strains. Additionally, enzyme activities were tested field-original highly resistant...

Abstract Long noncoding ribonucleic acids (lncRNAs) play crucial roles in regulating key biological processes; however, our knowledge of lncRNAs’ plant adaptive evolution is still limited. Here, we determined the divergence conserved lncRNAs closely related poplar species that were either tolerant or sensitive to salt stress by comparative transcriptome analysis. Among 34,363 identified lncRNAs, ~3% shared among with sequences but diversified their function, copy number, originating genomic...

Citrus Huanglongbing (HLB) is one of the most devastating citrus diseases worldwide. Sensitive and accurate assays are vital for efficient prevention spread HLB-associated "Candidatus Liberibacter spp". spp" that infect includes asiaticus" (Las), africanus" (Laf) americanus" (Lam). Of them, Las widespread species. In this study, a set nested PCR primer pairs were screened to diagnose Las, method greatly enhanced sensitivity detect up 10 times 100 compared qPCR conventional PCR, respectively....

A series of novel diterpenoids including imines, amides and ureas with a dehydroabietyl skeleton were screened to hepatocellular carcinoma (SMMC-7721), lung cancer (A-549), glioma (C-6) breast (MCF-7) tumor cells by MTT method. Their antitumor activity structure relationship analyzed. Several the title compounds such as I-2, I-10, I-6 I-5, possess noticeable against SMMC-7721, A-549, C-6 MCF-7 cells, lowest IC50 values 6.65, 0.75, 0.81 10.65μM, respectively. Based on structure-activity...

Soil salinity represents a major constraint in the growth of chrysanthemum. Therefore, improving tolerance chrysanthemum has become an important research direction breeding. Multiprotein bridging factor 1 (MBF1) is evolutionarily highly conserved transcriptional co-activator archaea and eukaryotes been reported to play roles respond abiotic stresses. Here, MBF1 gene induced by salt stress was isolated functionally characterized from Dendranthema grandiflorum name as DgMBF1. Overexpression...

Lily (Lilium spp.) is an important horticultural crop, but its use limited due to serious pollen contamination problems. There are many studies on development in model plants, few flower crops such as lilies. Gibberellin (GA) a large class of hormones and plays role plant vegetative growth reproductive development. GAMYB group the R2R3-MYB family upregulated by gibberellin, anther Here, we isolated novel GAMYB, named LoMYB65, from lily, which was closely related AtMYB65 AtMYB33 Arabidopsis....

Lily (Lilium spp.) is a widely cultivated horticultural crop that has high ornamental and commercial value but also the serious problem of pollen pollution. However, mechanisms anther dehiscence in lily remain largely unknown. In this study, morphological characteristics stomium zone (SZ) from different developmental stages ‘Siberia’ anthers were investigated. addition, transcriptomic metabolomic data analyzed to identify differentially expressed genes (DEGs) secondary metabolites involved...

The Asian citrus psyllid (ACP), Diaphorina citri (Kuwayama) (Hemiptera: Liviidae), is a notorious Rutaceae plant pest. Frequent and extensive use of pesticides has resulted in severe insecticide resistance ACP populations. Fully understanding the mechanism to vital for us control or delay development resistance. Therefore, we compared difference imidacloprid thiamethoxam between Hunan (Yongzhou, Chenzhou) Guangdong (Guangzhou) populations analyzed correlations level genes symbiotic fungi....

Speciation is a central topic in evolutionary biology. However, how genomic divergence originates and accumulates the face of gene flow during ecological adaptation remains poorly understood. Closely related species that have adapted to distinct environments but inhabit some overlapping ranges provide an ideal system evaluate this issue. Here, we combine population genomics distribution models (SDMs) examine divergences between two sister plant species, Medicago ruthenica M....