A5101475005- Influenza Virus Research Studies
- Plant Molecular Biology Research
- Animal Disease Management and Epidemiology
- Plant Virus Research Studies
- Genetic Mapping and Diversity in Plants and Animals
- Viral gastroenteritis research and epidemiology
- Immune Response and Inflammation
- Plant-Microbe Interactions and Immunity
- interferon and immune responses
- Plant Stress Responses and Tolerance
- Photosynthetic Processes and Mechanisms
- Plant Physiology and Cultivation Studies
- SARS-CoV-2 and COVID-19 Research
- Reproductive System and Pregnancy
- Plant Gene Expression Analysis
- Fungal Plant Pathogen Control
- Plant pathogens and resistance mechanisms
- Plant Genetic and Mutation Studies
- Research in Cotton Cultivation
- Viral Infections and Vectors
- Plant nutrient uptake and metabolism
- Immune Cell Function and Interaction
- Horticultural and Viticultural Research
- Plant and Fungal Interactions Research
- Plant Pathogens and Fungal Diseases
China Tobacco
2024
Huazhong Agricultural University
2007-2023
Nanjing Agricultural University
2012-2022
Chinese Academy of Sciences
2009-2021
University of Chinese Academy of Sciences
2021
State Key Laboratory of Agricultural Microbiology
2012-2021
Institute of Zoology
2019-2021
University of Nebraska–Lincoln
2018-2021
University of British Columbia
2020
Sainsbury Laboratory
2018
Most land plant genomes carry genes that encode RPW8-NLR Resistance (R) proteins. Angiosperms two subclasses: ADR1 and NRG1. ADR1s act as 'helper' NLRs for multiple TIR- CC-NLR R proteins in Arabidopsis. In angiosperm families, NRG1 co-occurs with TIR-NLR genes. We tested whether is required signalling of TIR-NLRs. Using CRISPR mutagenesis, we obtained an nrg1a-nrg1b double mutant Arabidopsis accessions, nrg1 Nicotiana benthamiana. These mutants are compromised all TIR-NLRs tested, including...
The photoperiodic response is one of the most important factors determining heading date in rice (Oryza sativa). Although rhythmic expression patterns flowering time genes have been reported to fine-tune response, posttranslational regulation key regulators has seldom elucidated rice. Heading 1 (Hd1) encodes a zinc finger transcription factor that plays crucial role which determines regional adaptability. However, little known about molecular mechanisms Hd1 accumulation during photoperiod...
Age is a risk factor for coronavirus disease 2019 (COVID-19) associated morbidity and mortality in humans; hence, this study, we compared the course of severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2) infection young aged BALB/c mice. We found that SARS-CoV-2 isolates replicated tracts 12-month-old (aged) mice caused pathological features pneumonia upon intranasal infection. In contrast, rapid viral clearance was observed 5 days following 2-month-old (young) with no evidence...
Abstract Resistance of benzimidazole fungicides is related to the point mutation β-tubulin gene in Sclerotinia sclerotiorum . The at codon 198 (GAG → GCG, E198A) occurs more than 90% field resistant populations China. Traditional detection methods benzimidazole-resistant mutants S. are time-consuming, tedious and inefficient. To establish a suitable rapid , an efficient simple method with high specificity was developed based on loop-mediated isothermal amplification (LAMP). Eight sets LAMP...
Abstract Background The point mutation at codon 200 ( TTC → TAC , F200Y ) of the β 2 ‐ tubulin gene confers resistance to benzimidazole fungicide in Fusarium asiaticum . These isolates with this have been detected mainly by determining minimum inhibitory concentration MIC fungicides, which is always time consuming, tedious and inefficient. Results A visual, rapid efficient method high specificity was developed, based on loop‐mediated isothermal amplification LAMP ). Six sets primers were...
An avian H10N5 influenza virus, A/swine/Hubei/10/2008/H10N5, was isolated from pigs in the Hubei Province of central China. Homology and phylogenetic analyses all eight gene segments demonstrated that strain wholly origin closely homologous to Eurasian lineage virus. To our knowledge, this is first report interspecies transmission an virus domestic under natural conditions.
Flowering time is one of the most important agronomic characteristics that ultimately determine yield potential and eco-geographical adaptation in crops. Ghd8 Ghd7, two major flowering genes, have similar functions large pleiotropic effects controlling heading date, plant height grain rice. However, these gene interactions at genetic molecular levels not been determined to date.In this study, we investigated interaction between Ghd7 by using a set near-isogenic lines panel natural germplasm...
Abstract Induced resistance is considered an eco-friendly disease control strategy, which can enhance plant by inducing the plant’s immune system to activate defense response. In recent years, studies have shown that lactic acid play a role in against biological stress; however, whether improve tobacco Phytophthora nicotianae, and its molecular mechanism remains unclear. our study, mycelial growth sporangium production of P. nicotianae were inhibited vitro dose-dependent manner. Application...
The M1 of influenza A virus (IAV) is important for the life cycle, especially assembly and budding viruses, which a multistep process that requires host factors. Identifying novel proteins interact with understanding their functions in IAV replication are great interest antiviral drug development. In this study, we identified 19 DF1 cells suspected to protein an H5N6 through immunoprecipitation (IP)/mass spectrometry. Among them, PSMD12, 26S proteasome regulatory subunit, was shown M1,...
Pigs are proposed to be "mixing vessel" hosts that can produce genetically novel reassortant viruses with pandemic potential. The appearance of any influenza among pigs should pose concerns for human health. Here, we report the complete genome sequence a H4N1 virus [A/Swine/HuBei/06/2009(H4N1)] isolated from pig in Central China 2009. genomic analysis indicates this is wholly avian-original virus. Each gene may come different avian outside mainland China, suggesting role migratory birds dispersal
Influenza A viruses are serious zoonotic pathogens that continuously cause pandemics in several animal hosts, including birds, pigs, and humans. Indole derivatives containing an indole core framework have been extensively studied developed to prevent and/or treat viral infection. This study evaluated the anti-influenza activity of derivatives, 3-indoleacetonitrile, indole-3-carboxaldehyde, 3-carboxyindole, gramine, A549 MDCK cells. Among these compounds, 3-indoleacetonitrile exerts profound...
Swine influenza A virus (swIAV) is a major concern for the swine industry owing to its highly contagious nature and acute viral disease. Currently, most commercial swIAV vaccines are traditional inactivated vaccines. The Lactobacillus plantarum-based vaccine platform promising approach mucosal development. Oral intranasal immunisations have potential induce immune response, which confers protective immunity. aim of this study was evaluate probiotic adhesion ability three L. plantarum...
The point mutation at codon 200 (TTC→TAC, F200Y) confers moderate resistance to carbendazim in Sclerotinia sclerotiorum. This mutant genotype (F200Y) has been detected mainly by determining the minimum inhibitory concentration (MIC), which requires 3 5 days. Here, we developed a loop-mediated isothermal amplification (LAMP) assay for rapid detection of F200Y carbendazim-resistant isolates S. Specific LAMP primers were designed and concentrations components optimized. optimal reaction...
Abstract Variations in the gene promoter play critical roles evolution of important adaptive traits crops, but direct links regulatory mutation to change are not well understood. Here, we examine nucleotide variations region a transcription factor ( Ghd8 ) that control grain number, plant height and heading date rice. We find dominant type subspecies japonica displayed high activity for expression comparison with one indica . Transgenic analyses revealed higher levels delayed enhanced cold...
Abstract Both genetic and epigenetic information must be transferred from mother to daughter cells during cell division. The mechanisms through which about chromatin states marks like histone 3 lysine 27 trimethylation (H3K27me3) are have been characterized in animals; these processes less well understood plants. Here, based on characterization of a dwarf rice (Oryza sativa) mutant (dwarf-related wd40 protein 1, drw1) deficient for yeast CTF4 (CHROMOSOME TRANSMISSION FIDELITY PROTEIN 4), we...
In this study, an immunochromatographic strip (ICS) was developed for the detection of antibody against Streptococcus suis serotype 2 (SS2). Colloidal gold particles labeled with staphylococcal protein A (SPA), which can bind to F C fragment mammalian immunoglobulin, were used as detector reagent. The capsular polysaccharide (CPS) SS2 and affinity-purified IgG from a healthy naive pig immobilized on test control regions nitrocellulose membrane, respectively. ICS 1) detect anti-CPS in 14 sera...
Tobacco black shank (TBS), caused by Phytophthora nicotianae, is one of the most harmful diseases tobacco. There are many studies have examined mechanism underlying induction disease resistance arbuscular mycorrhizal fungi (AMF) and β-aminobutyric acid (BABA) alone, but synergistic effects AMF BABA on not yet been studied. This study application inoculation immune response to TBS in The results showed that spraying leaves could increase colonization rate AMF, index tobacco infected...
We reported the complete genome sequence of an H5N5 avian influenza virus (AIV) that was first isolated from duck in central China 2010. Genomic and phylogenetic analyses showed this a recombinant between H5N1 AIV circulated southeastern Asia N5 subtype virus. These data are beneficial for investigating epidemiology ecology AIVs China.