A5045429163- Plant Molecular Biology Research
- Genetic Mapping and Diversity in Plants and Animals
- Chromosomal and Genetic Variations
- Plant Reproductive Biology
- Plant tissue culture and regeneration
- Plant nutrient uptake and metabolism
- Genomics and Phylogenetic Studies
- Plant Disease Resistance and Genetics
- Photosynthetic Processes and Mechanisms
- Plant responses to water stress
- Plant Genetic and Mutation Studies
- Plant Virus Research Studies
- Plant Gene Expression Analysis
- CRISPR and Genetic Engineering
- GABA and Rice Research
- Polysaccharides and Plant Cell Walls
- Rice Cultivation and Yield Improvement
- Soybean genetics and cultivation
- Legume Nitrogen Fixing Symbiosis
- DNA Repair Mechanisms
- biodegradable polymer synthesis and properties
- Ubiquitin and proteasome pathways
- Plant biochemistry and biosynthesis
- Plant Micronutrient Interactions and Effects
- Light effects on plants
State Key Laboratory of Food Science and Technology
2024-2025
Jiangnan University
2024-2025
Huazhong Agricultural University
2014-2024
Institute of Vegetables and Flowers
2024
Chinese Academy of Agricultural Sciences
2024
Louisiana State University Health Sciences Center New Orleans
2014
Nanjing University
2000
Phosphate (Pi) homeostasis in plants is required for plant growth and development, achieved by the coordination of Pi acquisition, translocation from roots to shoots, remobilization within plants. Previous reports have demonstrated that over-expression OsPHR2 (the homolog AtPHR1) knockdown OsSPX1 result accumulation excessive shoot rice. Here we report positively regulates low-affinity transporter gene OsPT2 physical interaction upstream regulation OsPHO2 roots. responsible most...
Asian cultivated rice consists of two subspecies: Oryza sativa subsp. indica and O. japonica Despite the fact that accounts for over 70% total production worldwide is genetically much more diverse, a high-quality reference genome has yet to be published. We conducted map-based sequencing lines, Zhenshan 97 (ZS97) Minghui 63 (MH63), which represent major varietal groups subspecies are parents an elite Chinese hybrid. The sequences were assembled into 237 181 (MH63) contigs, with accuracy...
Summary Enhancer trapping has provided a powerful strategy for identifying novel genes and regulatory elements. In this study, we adopted an enhancer trap system, consisting of the GAL4/VP16–UAS elements with GUS as reporter, to generate population rice. Currently, 31 443 independent transformants were obtained from two cultivars using Agrobacterium ‐mediated T‐DNA insertion. PCR tests DNA blot hybridization showed that about 94% contained insertions. The carried, on average, copies T‐DNA,...
Transition from the vegetative phase to reproductive is a crucial process in life cycle of higher plants. Although molecular mechanisms flowering regulation have been extensively characterized number plant species, little known regarding how transition initiates. Here, we show that Rice Indeterminate 1 ( RID1 ) gene acts as master switch for phase. encodes Cys-2/His-2-type zinc finger transcription factor does not an ortholog Arabidopsis spp. A knockout rid1 ), mutated by T-DNA insertion,...
Summary Rice is a major food crop with enormous biomass residue for biofuels. As plant cell wall recalcitrance basically decides costly process, genetic modification of walls has been regarded as promising solution. However, due to structural complexity and functional diversity walls, it becomes essential identify the key factors modifications that could not much alter growth, but cause an enhancement in enzymatic digestibility. To address this issue, we performed systems biology analyses...
Abstract Programmed cell death (PCD) during tapetum degeneration in postmeiotic anthers is critical for the proper development of male gametophytes flowering plants. Although several genes involved this process have been identified recently, molecular mechanism still poorly understood. Here, we show that knockout rice (Oryza sativa) APOPTOSIS INHIBITOR5 (API5), which encodes a putative homolog antiapoptosis protein Api5 animals, results delayed due to inhibition tapetal PCD leading defects...
Summary Rice tiller angle determines plant growth density and further contributes grain production. Although a few genes have been characterized to regulate in rice, the molecular mechanism underlying control of via microRNA is poorly understood. Here, we report that rice controlled by OsmiR167a‐targeted auxin response factors OsARF12 , OsARF17 Os ARF25 . In overexpression OsMIR167a plants, expression was severely repressed displayed larger as well osarf12/osarf17 osarf12/ osarf25 plants....
Panicle architecture is a key determinant of rice grain yield and mainly determined at the 1-2 mm young panicle stage. Here, we investigated transcriptome panicles from 275 varieties identified thousands genes whose expression levels were associated with traits. Multimodel association studies suggested that many small-effect genetic loci determine spikelet per (SPP) by regulating We found alleles cis-expression quantitative trait SPP-associated underwent positive selection, strong preference...
Abstract Background Epigenetic marks are reprogrammed during sexual reproduction. In flowering plants, DNA methylation is only partially remodeled in the gametes and zygote. However, timing functional significance of remodeling plant gametogenesis remain obscure. Results Here we show that starts after male meiosis rice, with non-CG methylation, particularly at CHG sites, being first enhanced microspore subsequently decreased sperm. Functional analysis rice methyltransferase genes CMT3a CMT3b...
We isolated 13 804 T-DNA flanking sequence tags (FSTs) from a insertion library of rice. A comprehensive analysis the FSTs revealed number features demonstrating highly non-random distribution insertions in rice genome: were biased towards large chromosomes, not only absolute but also relative density; within chromosomes occurred more densely distal ends, and less centromeric regions; was correlated with that full-length cDNAs, correlations heterogeneous among chromosomes; strongly...
Abstract CONSTANS, CONSTANS-LIKE, and TIMING OF CAB EXPRESSION1 (CCT) domain-containing proteins are a large family unique to plants. They transcriptionally regulate photoperiodic flowering, circadian rhythms, vernalization, other related processes. Through their CCT domains, CONSTANS HEADING DATE1 (HD1) coordinate with the NUCLEAR FACTOR Y (NF-Y) B/C dimer specifically target conserved ‘CCACA’ motif within promoters of genes. However, mechanism underlying DNA recognition by domain remains...
The photoperiodic response is one of the most important factors determining heading date in rice (Oryza sativa). Although rhythmic expression patterns flowering time genes have been reported to fine-tune response, posttranslational regulation key regulators has seldom elucidated rice. Heading 1 (Hd1) encodes a zinc finger transcription factor that plays crucial role which determines regional adaptability. However, little known about molecular mechanisms Hd1 accumulation during photoperiod...
Abstract Replication protein A (RPA), a highly conserved single-stranded DNA-binding in eukaryotes, is stable complex comprising three subunits termed RPA1, RPA2, and RPA3. RPA required for multiple processes DNA metabolism such as replication, repair, homologous recombination yeast (Saccharomyces cerevisiae) human. Most eukaryotic organisms, including fungi, insects, vertebrates, have only single gene that encodes each subunit. Arabidopsis (Arabidopsis thaliana) rice (Oryza sativa),...
Meiosis is essential for eukaryotic sexual reproduction and important genetic diversity among individuals. Although a number of genes regulating homologous chromosome pairing synapsis have been identified in the plant kingdom, their molecular basis remains poorly understood. In this study, we novel gene, PAIR3 (HOMOLOGOUS PAIRING ABERRATION IN RICE MEIOSIS 3), required rice. Two independent alleles, designated pair3-1 pair3-2, were our T-DNA insertional mutant library which could not form...
Heading date and grain weight are two determining agronomic traits of crop yield. To date, molecular factors controlling both heading have not been identified. Here we report the isolation a hemizygous mutation, (hgw), which delays reduces in rice. Analysis hgw mutant phenotypes indicate that mutation decreases latitudinal cell number lemma palea, composing spikelet hull is known to determine size shape brown grain. Molecular cloning characterization HGW gene showed it encodes novel...