A5020136297- Echinoderm biology and ecology
- Identification and Quantification in Food
- Fish Biology and Ecology Studies
- Coral and Marine Ecosystems Studies
- Environmental DNA in Biodiversity Studies
- Aquatic life and conservation
- Genomics and Phylogenetic Studies
- Genetic diversity and population structure
- Microbial Community Ecology and Physiology
- Marine Bivalve and Aquaculture Studies
- Marine and coastal plant biology
- Ichthyology and Marine Biology
- Glioma Diagnosis and Treatment
- Marine Ecology and Invasive Species
- Marine and fisheries research
- Single-cell and spatial transcriptomics
- Olfactory and Sensory Function Studies
- Marine animal studies overview
- Animal Genetics and Reproduction
- Microtubule and mitosis dynamics
- Methane Hydrates and Related Phenomena
- Aquaculture Nutrition and Growth
- Aquatic Ecosystems and Biodiversity
- Biochemical Analysis and Sensing Techniques
- Crustacean biology and ecology
University of the Ryukyus
2015-2024
National Institute of Oceanography and Fisheries
2015-2024
St. Jude Children's Research Hospital
2024
Okinawa Institute of Science and Technology Graduate University
2016-2022
Arab Academy for Science, Technology, and Maritime Transport
2021
Kafrelsheikh University
2017
University of Toyama
2012-2014
Structure and diversity of microbial communities are an important research topic in biology, since microbes play essential roles the ecology various environments. Different DNA isolation protocols can lead to data bias affect results next-generation sequencing. To evaluate impact for from soil samples also influence individual handling samples, we compared obtained by two researchers (R T) using different extraction kits: (1) MO BIO PowerSoil® Isolation kit (MO_R MO_T) (2) NucleoSpin® Soil...
Recently, sea cucumbers (Echinodermata: Holothuroidea) have been over-exploited in many areas of the world, including Ryukyu Islands, southern Japan, due to increases their economic importance. Nevertheless, management and protection are insufficient worldwide. The black cucumber Holothuria ( Halodeima ) atra Jaeger, 1833, inhabits a large range across Indo-West Pacific Ocean is widely harvested species. Here we conducted population genetic analyses on H . using partial mitochondrial DNA...
Background: The family Sparidae includes about 115 species divided into 33 genera that inhabits tropical and temperate coastal waters of the Pacific, Indian Atlantic oceans.Herein, fish were molecularly barcoded using mitochondrial gene, cytochrome oxidase subunit I (COI).Materials Methods: Fish samples from 22 collected Abo Qir Bay, West Mediterranean sea Gulf Suez, North Red Sea.Genomic DNA was extracted muscles these partial coding region COI gene amplified then sequenced their sequences...
Abstract Recently, tilapia have become increasingly important in aquaculture and fisheries worldwide. They are one of the major protein sources many African countries helping to combat malnutrition. Therefore, maintenance conservation genetics wild populations great significance. In this study, we report population genetic structure diversity redbelly ( Coptodon zillii ) three different Egyptian aquatic environments: brackish (Lake Idku), marine (Al‐Max Bay), freshwater Nasser). The habitat...
Africa is a continent that incubates huge inland freshwater reservoirs, representing 27% of total world liquid freshwater, and bounded by marine blue belt. This topography makes harboring diverse fishery resources. However, fisheries in are still facing big challenges can be resolved molecular genetic tools. The present review demonstrated how DNA barcoding approaches expand African to solve the problems fish species identifications, evolutions, population differentiations biogeographic...
Microbial community diversity and chemodiversity were investigated in marine sediments adjacent to the Okinawan "Kaichu-Doro" Causeway, which was constructed 46 years ago connect a group of four islands (Henza-jima, Miyagi-jima, Ikei-jima, Hamahiga-jima) main island. This causeway not built on pilings, but by land reclamation; hence, it now acts as long, thin peninsula. The construction this previously shown have influenced surrounding ecosystem, causing ecosystem fragmentation loss water...
Structure and diversity of microbial communities are an important research topic in biology, since microbes play essential roles the ecology various environments. Different DNA isolation protocols can lead to data bias affect results next-generation sequencing. To evaluate impact for from soil samples also influence individual handling samples, we compared obtained by two researchers (R T) using different extraction kits: (1) MO BIO PowerSoil ® Isolation kit (MO_R MO_T) (2) NucleoSpin Soil...
Phylogenetic comparison for lessepsian puffer fish Lagocephalus sceleratus (Gmelin, 1789) from the Egyptian waters (Mediterranean and Red Seas) was performed using morphological genetic characteristics to confirm its identification recognize any variations due differences in both habitats. A similar identity body description habitats observed. Slight phenotyping some measurements were observed with no significant difference. The correlation between reference length most of indices (0.99)...
The greenfish sea cucumber Stichopus chloronotus is an economically and ecologically important species throughout its range. This widely distributed, inhabiting coral reefs of the Indo-Pacific Ocean. Our study evaluated population genetic structure levels diversity in southern Japan. A total 180 individuals were collected from eight locations Okinawa Okinoerabu Islands sequenced using mitochondrial 16S ribosomal DNA (16S) nuclear histone H3 (H3) gene. Only three haplotypes detected (518 bp)...
In sensory systems of the brain, mechanisms exist to extract distinct features from stimuli generate a variety behavioral repertoires. These often correspond different cell types at various stages in processing. mammalian olfactory system, complex information processing starts bulb, whose output is conveyed by mitral cells (MCs) and tufted (TCs). Despite many differences between them, despite crucial position they occupy hierarchy, Cre-driver lines that distinguish them do not yet exist....
Recurring outbreaks of crown-of-thorns starfish (COTS) severely damage healthy corals, especially in the Western Pacific Ocean. To obtain a better understanding population genetics COTS and historical colonization across Ocean, complete mitochondrial genomes were sequenced from 243 individuals collected 11 reef regions. Our results indicate that ( Acanthaster cf. solaris ) comprise two major clades, an East-Central (ECP) clade Pan-Pacific (PP) clade, separation which was supported by high...
Species of family Sparidae, commonly called, sea breams, are widely distributed from temperate to tropical waters and great economic interest.However, in Egypt, limited data is available on genetic variation evolutionary relationships Sparidae.Therefore, the study diversity among sparid species crucial for proper management convenient strategies.The aim this was evaluate diversification 22 belonging Sparidae Mediterranean Sea Red Egypt using three different molecular markers.DNA barcoding,...
Scoliorhapis dianthus, new species, was collected from depths of 0.20–1 m in the Sea Japan. It is distinctive having ten tentacles with six pairs digits equal size. Ossicles body wall have a sigmoid shape and average 75 ± 5 μm length. An identification key to all species genus provided.
In this study we investigated the phylogenetics of two sea urchin species, Arbacia lixula and Paracentrotus lividus from Mediterranean Sea. Specimens were collected east coast Alexandria City, Egypt. Pigmentation examination showed four sympatric color morphotypes (black, purple, reddish brown, olive green). Mitochondrial DNA was extracted specimens mitochondrial cytochrome oxidase subunit I (COI) 16S ribosomal RNA (16S) sequenced. The results that all black constituted species A. lixula....
Sea cucumbers are important ecological engineers in marine ecosystems. However, the fishery demand of some species, especially large-epifaunal and commercially used (LEC) sea cucumbers, has risen drastically, resulting serious depletion local populations for many species. Despite this problem, basic data on such as population densities preferred habitats, often still insufficient. Here, we report multiple LEC cucumber their ambient benthic communities at eight sites around Okinawa Islands....
Culture-independent molecular study presented the first exploring and evaluation of prokaryotic communities in two desert brines; athalassohaline Lake "Aghormy", Siwa Oasis, Western Desert Egypt, thalassohaline saltern "Sebeaka Sabkha", Bardawil Lagoon, north coast Sinai. 16S rRNA gene metabarcoding generated a total 488,828 reads from both sites, grouped into 17,741 operational taxonomic units (OTUs). 3030 OTUs were shared while 2255 9426 unique to Aghormy Sebeaka Sabkha, respectively. Both...
This study evaluated how water temperature (26, 28, and 30°C), number of meals per day (one or two meals), protein percent in diet (20, 25 30%) impact growth performance, biometric indices, feeding behavior Nile tilapia, Oreochromis niloticus. Fish were randomly allocated into 18 equal replicate groups. Higher final body weight was observed fish reared at 30°C fed one meal containing 30% crude protein. Better gain, gain %, feed conversion ratio, specific rate, condition factor recorded 26°C...
An unexpected new Metapenaeus shrimp species has arrived to the coastal water of Alexandria City, Egypt. Morphological and molecular studies were conducted for its identification. The results revealed that it is Indo-West Pacific species, affinis (H. Milne Edwards, 1837), which considered as a addition already present local penaeidae species; monoceros (Fabricius, 1798) stebbingi (Nobili, 1904). Large numbers observed collected by Egyptian fishermen from waters Egypt, during study period...
We examined the genetic diversity and connectivity of two indigenous Mediterranean goatfish species (Mullus barbatus M. surmuletus), a Lessepsian migrant (Upeneus moluccensis), across Nile Delta outflow using mitochondrial DNA markers (COI cyt b). Genetic was high for but relatively lower species, suggesting founder effects after invasion from Red Sea. Confirmation this hypothesis, however, would require comparison with populations origin in Sea broader Indo-West Pacific. AMOVA network...