A5038366105- Molecular Biology Techniques and Applications
- RNA Research and Splicing
- RNA Interference and Gene Delivery
- Single-cell and spatial transcriptomics
- Cancer Genomics and Diagnostics
- RNA and protein synthesis mechanisms
- Cancer-related molecular mechanisms research
- CRISPR and Genetic Engineering
- Transgenic Plants and Applications
- Gene expression and cancer classification
- Advanced biosensing and bioanalysis techniques
- Plant tissue culture and regeneration
- Insect symbiosis and bacterial influences
- RNA modifications and cancer
- Bacteriophages and microbial interactions
- Viral Infections and Immunology Research
- Plant Virus Research Studies
Broad Institute
2021-2024
Massachusetts Institute of Technology
2021-2024
University of California, Berkeley
2017-2019
Significance Plant bioengineering will be necessary to sustain plant biology and agriculture, where the delivery of biomolecules such as DNA, RNA, or proteins cells is at crux biotechnology. Here, we show that DNA nanostructures can internalize into deliver siRNA mature tissues without external aid. Furthermore, demonstrate nanostructure size, shape, compactness, stiffness affect both internalization subsequent gene silencing efficiency. Interestingly, also find attachment locus affects...
The precise control of messenger RNA (mRNA) translation is a crucial step in posttranscriptional gene regulation cellular physiology. However, it remains challenge to systematically study mRNA at the transcriptomic scale with spatial and single-cell resolution. Here, we report development ribosome-bound mapping (RIBOmap), highly multiplexed three-dimensional situ profiling method detect translatome. RIBOmap 981 genes HeLa cells revealed cell cycle-dependent translational colocalized...
Abstract Quantifying RNAs in their spatial context is crucial to understanding gene expression and regulation complex tissues. In situ transcriptomic methods generate spatially resolved RNA profiles intact However, there a lack of unified computational framework for integrative analysis data. Here, we introduce an unsupervised annotation-free framework, termed ClusterMap, which incorporates the physical location identity RNAs, formulates task as point pattern problem, identifies biologically...
Abstract Type III-A CRISPR-Cas systems are prokaryotic RNA-guided adaptive immune that use a protein-RNA complex, Csm, for transcription-dependent immunity against foreign DNA. Csm can cleave RNA and single-stranded DNA (ssDNA), but whether it targets one or both nucleic acids during transcription elongation is unknown. Here, we show binding of Thermus thermophilus (T . ) (TthCsm) to nascent transcript in complex (TEC) promotes tethering not direct contact TthCsm with polymerase (RNAP)....
mRNA has recently been established as a new class of therapeutics, due to its programmability and ability produce proteins interest rapidly in vivo. Despite demonstrated utility, protein expression platform remains limited by translational capacity RNA stability. Here, we introduce messenger-oligonucleotide conjugated RNAs (mocRNAs) enable site-specific, robust, modularized encoding chemical modifications for highly efficient stable expression. In mocRNA constructs, chemically synthesized...
Genetic engineering of plants is at the core sustainability efforts, natural product synthesis, and agricultural crop engineering. The plant cell wall a barrier that limits ease throughput with which exogenous biomolecules can be delivered to plants. Current delivery methods either suffer from host range limitations, low transformation efficiencies, tissue damage, or unavoidable DNA integration into genome. Here, we demonstrate efficient diffusion-based biomolecule tissues organs intact...
Abstract The precise control of mRNA translation is a crucial step in post-transcriptional gene regulation cellular physiology. However, it remains major challenge to systematically study at the transcriptomic scale with spatial and single-cell resolutions. Here, we report development RIBOmap, three-dimensional (3D) situ profiling method detect for thousands genes simultaneously intact cells tissues. By applying RIBOmap 981 HeLa cells, revealed remarkable dependency on cell-cycle stages...
Abstract Quantifying RNAs in their spatial context is crucial to understanding gene expression and regulation complex tissues. In situ transcriptomic methods generate spatially resolved RNA profiles intact However, there a lack of unified computational framework for integrative analysis data. Here, we present an unsupervised annotation-free framework, termed ClusterMap, which incorporates physical proximity identity RNAs, formulates the task as point pattern problem, thus defines...
Abstract Quantifying RNAs in their spatial context is crucial to understanding gene expression and regulation complex tissues. In situ transcriptomic methods generate spatially resolved RNA profiles intact However, there a lack of unified computational framework for integrative analysis data. Here, we present an unsupervised annotation-free framework, termed ClusterMap, which incorporates physical proximity identity RNAs, formulates the task as point pattern problem, thus defines...
Abstract Plant bioengineering may generate high yielding and stress-resistant crops amidst a changing climate growing global population (1–3). However, delivery of biomolecules to plants relies on Agrobacterium infection (4) or biolistic particle (5), the former which is only amenable DNA delivery. The difficulty in delivering functional such as RNA plant cells due cell wall absent mammalian poses dominant physical barrier exogenous biomolecule plants. nanostructure-mediated an effective...