A5089922839- Advanced biosensing and bioanalysis techniques
- DNA and Nucleic Acid Chemistry
- RNA Interference and Gene Delivery
- RNA and protein synthesis mechanisms
Changzhi Medical College
2021-2025
Significance Our work reveals the genome-wide prevalence of G4 structural variations (G4Vs) associated with single-nucleotide (SNVs) in human genome and how such G4Vs interact gene-regulatory elements subsequently affect activities genes accommodating G4Vs. The are enriched around transcription start sites, implying their potential involvement gene expression. Because structures participate essential physiological pathological processes, should make crucial contributions to genotype...
Abstract DNA-protein interactions at gene promoters play a critical role in expression. The of human cells are highly enriched guanine-rich sequences, which can form four-stranded G-quadruplex (G4) structures. G4s emerging as distinct class structure-based regulatory elements regulation, and their interaction with proteins is essential for the play. Currently, our understanding G4-protein mainly on case-by-case basis, without systematic information. In this work, we examined spatial...
Guanine-rich DNA forms G-quadruplexes (G4s) that play a critical role in essential cellular processes. Previous studies have mostly focused on intramolecular G4s composed of four consecutive guanine tracts (G-tracts) from single strand. However, this structural form has not been strictly confirmed the genome living eukaryotic cells. Here, we report formation hybrid (hG4s), consisting G-tracts both and RNA, yeast Analysis Okazaki fragment syntheses two other independent G4-specific detections...
Abstract Guanine-rich nucleic acids form G-quadruplex (G4) structures that play a critical role in cellular processes. Previous studies have mostly focused on monomeric intramolecular G4s with four consecutive guanine tracts (G-tracts) from single strand. However, this structural has never been confirmed eukaryotic cells. Here, we report the formation of hybrid (hG4s), consisting G-tracts both DNA and RNA, genome living yeast Analysis Okazaki fragment syntheses G4-specific probing reveals...