A5000214386- Photosynthetic Processes and Mechanisms
- Algal biology and biofuel production
- Plant biochemistry and biosynthesis
- Antioxidant Activity and Oxidative Stress
- Plant Stress Responses and Tolerance
- Microbial Community Ecology and Physiology
- Light effects on plants
- Cancer Research and Treatments
- Marine and coastal ecosystems
- Agriculture, Soil, Plant Science
- Lipid metabolism and biosynthesis
- Marine Toxins and Detection Methods
- Plant Gene Expression Analysis
- Earthquake and Tsunami Effects
- Plant nutrient uptake and metabolism
- Earthquake and Disaster Impact Studies
- Catalytic Processes in Materials Science
- Marine and fisheries research
- Advanced biosensing and bioanalysis techniques
- Spectroscopy and Chemometric Analyses
- Protist diversity and phylogeny
- Geotechnical Engineering and Soil Stabilization
- Porphyrin Metabolism and Disorders
- Electrochemical Analysis and Applications
- Plant responses to water stress
Rakuno Gakuen University
2025
Yokohama City University
1996-2024
Nikko Memorial Hospital
2022-2023
Hiroshima University
2010-2022
Sapporo Medical University
2021-2022
Kumamoto University
1955-2019
Nagasaki Prefectural Nagasaki Technical High School
2016-2019
Hokkaido Research Organization
2007-2017
Akita Prefectural University
2006-2016
Ishikawa Prefectural University
2016
Chlorophyllase (Chlase) is the first enzyme involved in chlorophyll (Chl) degradation and catalyzes hydrolysis of ester bond to yield chlorophyllide phytol. In present study, we isolated Chlase cDNA. We synthesized degenerate oligo DNA probes based on internal amino acid sequences purified from Chenopodium album , screened C. cDNA library, cloned a ( CaCLH chlorophyll-chlorophyllido hydrolase). The deduced sequence (347 aa residues) had lipase motif overlapping with an ATP/GTP-binding...
In Arabidopsis , monogalactosyldiacylglycerol (MGDG) is synthesized by a multigenic family of MGDG synthases consisting two types enzymes differing in their N-terminal portion: type A (atMGD1) and B (atMGD2 atMGD3). The present paper compares isoforms with the that are known to sit inner membrane plastid envelope. occurrence 16:3 18:3 plants shows both not specialized for prokaryotic eukaryotic glycerolipid biosynthetic pathways. Type atMGD1 gene abundantly expressed green tissues along...
Purine catabolism is regarded as a housekeeping function that remobilizes nitrogen for plant growth and development. However, emerging evidence suggests certain purine metabolites might contribute to stress protection of plants. Here, we show in Arabidopsis, the intermediary metabolite allantoin plays role abiotic tolerance via activation abscisic acid (ABA) metabolism. The aln loss-of-function ALN, encoding allantoinase, results increased accumulation, genome-wide up-regulation...
Allantoin is a metabolic intermediate of purine catabolism that often accumulates in stressed plants. Recently, we used Arabidopsis knockout mutants (aln) ALLANTOINASE to show this metabolite activates abscisic acid (ABA) production, thereby stimulating stress-related gene expression and enhancing seedling tolerance abiotic stress. A detailed re-examination the microarray data an aln mutant (aln-1) confirmed increased ABA-related genes also revealed altered involved jasmonic (JA) responses,...
The yeast Candida utilis does not possess an endogenous biochemical pathway for the synthesis of carotenoids. central isoprenoid concerned with prenyl lipids is present in C. and active biosynthesis ergosterol. In our previous study, we showed that introduction exogenous carotenoid genes, crtE, crtB, crtI, responsible formation lycopene from precursor farnesyl pyrophosphate, results strain yields at 1.1 mg per g (dry weight) cells (Y. Miura, K. Kondo, T. Saito, H. Shimada, P. D. Fraser, N....
The food-grade yeast Candida utilis has been engineered to confer a novel biosynthetic pathway for the production of carotenoids such as lycopene, beta-carotene, and astaxanthin. exogenous carotenoid biosynthesis genes were derived from epiphytic bacterium Erwinia uredovora marine Agrobacterium aurantiacum. individually modified based on codon usage C. glyceraldehyde 3-phosphate dehydrogenase gene expressed in under control constitutive promotes terminators utilis. resultant strains...
PURPOSE: Sexual dysfunction after surgery of the rectum is a serious complication to male patients. Autonomic nerve-preserving operation for rectal cancer has been performed within recent ten years maintain urinary and sexual functions without spoiling therapeutic radicality. To clarify function as degree autonomic operation, was outlined through clinical interview. METHOD: In series 134 patients who were undergoing cancer, detailed history postoperative obtained by interviews. RESULTS: 87.7...
A eubacteria-type RNA polymerase (PEP) plays crucial roles for chloroplast development in higher plants. The core subunits are encoded on plastid DNA (rpo genes) while the regulatory sigma factors nuclear (SIG genes). However, definite gene specificity of each factor is unknown. We recently identified an Arabidopsis recessive pale-green mutant abc1 which T-DNA inserted SIG2 (sigB). In this mutant, almost normal etioplasts were developed under dark conditions small chloroplasts with poor...
In Arabidopsis thaliana , we identified a novel gene of NADPH‐protochlorophyllide oxidoreductase (POR) isoform, which catalyzes the light‐dependent protochlorophyllide reduction in chlorophyll (Chl) biosynthetic pathway. The deduced amino acid sequence POR isoform (PORC) showed significant identities (∼75%) with previously isolated two isoforms A. . Contrasting these isoforms, PORC transcript increased etiolated seedlings by illumination, and was dominantly expressed immature mature tissues....
The arc3 (accumulation and replication of chloroplast) mutant Arabidopsis thaliana has a small number abnormally large chloroplasts in the cell, suggesting that chloroplast division is arrested ARC3 an important role initiation division. To elucidate ARC3, first we identified gene, determined location protein during because localization spatial orientation such factors are vital for correct Sequencing analysis showed was fusion prokaryotic FtsZ part eukaryotic...
NADPH:protochlorophyllide oxidoreductase (POR) catalyzes the light-dependent reduction of protochlorophyllide. To elucidate physiological function three differentially regulated POR isoforms (PORA, PORB and PORC) in Arabidopsis thaliana, we isolated T-DNA tagged null mutants porB porC. The mature seedlings had normal photosynthetic competencies, showing that PORC are interchangeable functionally redundant developed plants. In etiolated seedlings, only showed a photoactive protochlorophyllide...
This is the first report to explore fine‐scale diversity, population genetic structure, and biogeography of a typical planktonic microbe in Japanese Korean coastal waters also try detect impact natural human‐assisted dispersals on structure gene flow toxic dinoflagellate species. Here we present analysis Alexandrium tamarense (Lebour) Balech populations from 10 sites along coasts. We used nine microsatellite loci, which varied widely number alleles diversity across populations. The revealed...
Imperforate tracheary elements (ITEs) in wood of vessel-bearing angiosperms may or not transport water. Despite the significance hydraulic for defining ITE types, combination cell structure with water visualization planta has received little attention. This study provides a quantitative analysis structural features associated conductive vs. non-conductive nature ITEs. Visualization was studied 15 angiosperm species by dye injection and cryo-scanning electron microscopy. Structural ITEs were...
The phytohormone abscisic acid (ABA) is produced via a multistep de novo biosynthesis pathway or single-step hydrolysis of inactive ABA-glucose ester (ABA-GE). reaction catalyzed by β-glucosidase (BG, BGLU) isoforms localized to various organelles, where they become activated upon stress, but the mechanisms underlying this organelle-specific activation remain unclear. We investigated relationship between subcellular distribution and stress-induced BGLU18 (BG1), an endoplasmic reticulum...
Abstract Chloroplast development in cotyledons differs a number of ways from that true leaves, but the cotyledon-specific program chloroplast biogenesis has not been clarified. The cyo1 mutant Arabidopsis thaliana albino normal green leaves. Chloroplasts develop abnormally plants grown light, etioplasts are mutants dark. We isolated CYO1 by T-DNA tagging and verified allele was responsible for cotyledon phenotype complementation. C4-type zinc finger domain similar to Escherichia coli DnaJ....
We have previously demonstrated that RNA interference‐mediated suppression of xanthine dehydrogenase (XDH), the rate‐limiting enzyme in purine degradation, causes defects normal growth and development Arabidopsis thaliana . Here, we investigated a possible role for XDH drought tolerance, since this is also implicated plant stress responses acclimatization. When ‐suppressed lines were subjected to stress, was markedly reduced conjunction with significantly enhanced cell death H 2 O...
Intercellular CO2 concentration of leaves (Ci) is a critical parameter in photosynthesis. Nevertheless, uncertainties calculating Ci arise as stomata close. Here, by modifying the assimilation chamber commercial gas-exchange equipment to directly measure Ci, we demonstrate overestimation calculated (i.e. Ci(c)) without stimulating stomatal closure. Gas exchange was measured on one side leaf while (Ci(m)) acquired simultaneously other hypostomatous passion fruit (Passiflora edulis Sims) and...
Arbuscular mycorrhizal fungi, classified in the subphylum Glomeromycotina, are obligate symbionts that depend on photosynthetic products from plants. There is substantial evidence AMF support plant and crop growth natural agricultural ecosystems. Fine root endophytes (FRE) also co-occur roots with all but tropical environments. However, their presence remains poorly recognized, lifestyle functionality remain largely unknown. Our analysis demonstrates that, contrast to AMF, FRE colonize...
An in vitro assay procedure for the carotenoid (β‐ionone ring) 3,3′‐hydroxylase and 4,4′‐oxygenase has been developed that enables efficient conversion of non‐radiolabeled substrates added directly into aqueous solution. The following enzymic conversions were demonstrated apparent kinetic constants ( V max , K m specificity constants) obtained : (a) (from Agrobacterium aurantiacum Alcaligenes sp. strain PC‐1) converted phoenicoxanthin (adonirubin) to astaxanthin, 3‐hydroxyechinenone...