A5091053762- Plasma Applications and Diagnostics
- Plasma Diagnostics and Applications
- Electrohydrodynamics and Fluid Dynamics
- Surface Modification and Superhydrophobicity
- Plasma and Flow Control in Aerodynamics
- Mass Spectrometry Techniques and Applications
- Catalytic Processes in Materials Science
- Diamond and Carbon-based Materials Research
- Water Quality Monitoring and Analysis
- Aerosol Filtration and Electrostatic Precipitation
- Laser Design and Applications
- Hemoglobin structure and function
- Ion-surface interactions and analysis
- Analytical chemistry methods development
- Atmospheric chemistry and aerosols
- Carbon Nanotubes in Composites
- Radiation Effects and Dosimetry
- Graphene and Nanomaterials Applications
- Laser-induced spectroscopy and plasma
- Heme Oxygenase-1 and Carbon Monoxide
- Laser-Ablation Synthesis of Nanoparticles
- X-ray Spectroscopy and Fluorescence Analysis
- Laser Applications in Dentistry and Medicine
- Lattice Boltzmann Simulation Studies
- Computational Fluid Dynamics and Aerodynamics
Osaka Metropolitan University
2022-2025
Tokyo Metropolitan University
2022-2025
Osaka City University
2018-2023
Seoul National University
2022-2023
University of Illinois Urbana-Champaign
2023
Graduate School USA
2021
Meijo University
2017-2020
University of Liverpool
2011-2019
Kochi University of Technology
2007-2017
CeNTech
2015
We review new trends of agricultural applications atmospheric‐pressure plasmas for decontaminating products and enhancing seed germination growth plants beneficial microorganisms. classify the decontamination techniques into three kinds treatments, which are gas‐phase, in‐liquid, plasma‐activated liquid introduce recent studies mainly after 2010, discussing inactivation mechanism microorganisms in each treatment. Likewise, we categorize enhancement same enhancements detail, mechanisms future...
Abstract This paper presents and explains the principle behind anelectrochemical method to enhance antimicrobial action of plasma‐activated hydrogel therapy (PAHT) in context wound decontamination. The process involves grounding hydratingpoly(vinyl alcohol) (PVA) films during treatment with a helium (He) plasma jet. electrochemically enhances production hydrogen peroxide (H 2 O ), which is amajor antibacterial agent produced PVA hydrogel. Production H shownto be electrically enhanced through...
The efficacy of helium (He) and argon (Ar) plasma jets are being investigated for different healthcare applications including wound cancer therapy, sterilisation surface disinfections. Current research points to a potential link between the generation reactive oxygen nitrogen species (RONS) outcomes in range biological medical applications. As new data accrue, further strengthening this link, it becomes important understand controlled delivery RONS into solutions, tissue fluids tissues. This...
We report a simple experimental approach to follow the transport of helium (He) plasma-generated reactive oxygen and nitrogen species (RONS) through millimetre thick agarose targets. These RONS may be either primary RONS, generated directly by plasma jet, or secondary for example at surface of, within, material. Our experiment involves placing an film over quartz cuvette filled with deionized water. The is exposed He jet UV absorption profile (of water) recorded in real-time. Plasma exposure...
Plasma-activated water (PAW) is receiving much attention in biomedical applications because of its reported potent bactericidal properties. Reactive oxygen and nitrogen species (RONS) that are generated upon plasma exposure thought to be the key components PAW destroy bacterial cancer cells. In addition developing for PAW, it also necessary better understand RONS chemistry order tailor achieve a specific biological response. With this mind, we previously developed UV–vis spectroscopy method...
Using molecular beam mass spectroscopy, time-resolved measurements of the ionic species in plasma plume an atmospheric-pressure helium microplasma jet are made for a range excitation frequencies (5, 10 and 25 kHz) source–instrument orifice distances (1, 7 11 mm). Ionic can only be observed visible plume, with main positive being (65.26%) (21.11%), few percentages N + , O NO He . For negative ions, majority (22.68%), (H 2 O) (10.49%) large minority observed, namely clusters, (CO 3 ) The flux...
Cold atmospheric plasmas have attracted significant worldwide attention for their potential beneficial effects in cancer therapy. In order to further improve the effectiveness of plasma therapy, it is important understand generation and transport reactive species into tissue fluids, tissues cells, moreover rates depths delivery, particularly across physical barriers such as skin. this study, helium (He) jet treatment a 3D tumour, grown on back live mouse, induced apoptosis within tumour...
The influence of protein and molecular, ground state oxygen (O2) on the plasma generation, transport reactive nitrogen species (RONS) in tissue are investigated. A target, comprising a 1 mm thick gelatin film (a surrogate for real tissue), is placed top 96-well plate; each well filled with phosphate buffered saline (PBS, pH 7.4) containing one fluorescent or colorimetric reporter that specific three RONS (i.e., H2O2, NO2−, OH•) broad spectrum (2,7-dichlorodihydrofluorescein). helium cold...
Using Schlieren photography, the helium outflow configuration from a fine capillary-based microplasma jet discharge has been captured for free-stream conditions. The transition laminar to turbulent flow is clearly identified with and without operation of plasma. At rate 2.3 L·min <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">-1</sup> no plasma operating, this occurs 54 mm nozzle; however, struck (peak voltages 8 kV <sub...
A commercial current monitor has been used to measure the associated with plasma bullets created in both positive and negative half cycles of sinusoidal driving voltage sustaining a jet. The maximum values bullet are typically ∼750 µA persist for 10 µs, while peaks several hundred μA broad, persisting about 40 µs. From time delay increasing distance from jet nozzle, an average propagation speed measured; travel at 17.5 km s−1 3.9 km s−1 respectively. net space charge bullet(s) also...
Non-thermal atmospheric-pressure plasma (NTAPP) is attracting widespread interest for use in medical applications. The tissue repair capacity of NTAPP has been reported various fields; however, little known about its effect on fracture healing. Non-union or delayed union after a clinical challenge. In this study, we aimed to investigate how irradiation promotes healing non-union model and underlying mechanism, vitro vivo. For the vivo created normal models LEW/SsNSlc rats effects NTAPP. To...
A synthetic biological sensor was developed to monitor the interaction of plasma with soft, hydrated material. It comprises phospholipid vesicles in a proteinaceous environment comprising 5% (w/v) gelatin. The contained self-quenched dye, which activated by vesicle destruction giving clear fluorescent switch on. bacterial toxins measured proof principle experiment, then effect atmospheric jets sensor, studied order assess cytolytic systems. When contacted gelatin surface perpendicular...
We demonstrated the efficacy of using a simple experimental approach, involving UV absorption spectroscopy, to monitor transport reactive oxygen and nitrogen species (RONS) through an agarose film (as surrogate for real tissue) into deionized (DI) water. The experiment involved placing 4 mm thick over quartz cuvette filled with DI was exposed non-thermal, He atmospheric-pressure plasma jet (APPJ) water recorded in real-time. Our results indicate accumulation RONS within during APPJ exposure...
The mechanisms of plasma in medicine are broadly attributed to plasma-derived reactive oxygen and nitrogen species (RONS). In order exert any intracellular effects, these RONS must first traverse a major barrier the cell membrane. membrane lipid composition, thereby magnitude this barrier, is highly variable between cells depending on type state (e.g. it widely accepted that healthy cancerous have different compositions). study, we investigate how interactions with components can potentially...
Abstract To investigate the potential role of hydroxyl radical ( • OH) in cold atmospheric plasma (CAP) jet treatment, two fluorescence-based methodologies are utilised to measure DNA strand breaks. The first comprises a model system double-stranded oligomer, where respective ends tagged with fluorophore and quencher molecules; second, cell culture reporting breaks using γ -H2AX assay. During various CAP treatments, optical emission spectroscopy is used detect OH gas phase electron spin...
Background/Objective: Anti-tumor effects of plasma-activated medium (PAM) were demonstrated using various malignant tumors. However, the anti-tumor effect PAM on synovial sarcoma remains unclear. Therefore, we aimed to investigate and its underlying mechanisms, focusing quantitative analyses both intracellular reactive oxygen species (ROS) cell apoptosis. Methods: The human line HS-SY-II was used viability after treatment. We investigated side local injection in a xenograft murine model....
The helium (He) atmospheric-pressure plasma jet (APPJ) delivery of reactive oxygen and nitrogen species (RONS) molecular (O2) in deionized (DI) water was monitored real time using situ UV absorption spectroscopy. He APPJ used to treat DI directly through an agarose target as a surrogate for tissue (e.g., skin barrier). For direct treatment, the RONS were generated immediately water, concentration continued increase during treatment. But there only very minor total detected after gas flow...
We employed UV–vis spectroscopy to monitor real-time changes in the oxygen tension and concentration of reactive nitrogen species (RONS) deionized (DI) water during treatments with helium (He) argon (Ar) gas plasma jets. He Ar jets are both shown de-oxygenate DI being more efficient than Ar, whilst deliver regulate concentrations hydrogen peroxide (H2O2), nitrite (NO2−) nitrate (NO3−) water. The H2O2 NO3− production efficiency varied between jets, but was similar for NO2−. Whilst fully...
Abstract The plasma jet generation of reactive oxygen and nitrogen species (RONS) in solution is important biology, medicine, disinfection. Studies using a wide variety devices have been carried out for this purpose, making it difficult to compare the performance between devices. In study, we compared efficiency RONS deionized (DI) water 3.7-mm- 800-µm-sized helium (He) jets (hereafter mm-jet µm-jet, respectively) at different treatment distances times. was determined by considering total...
Abstract We treat deionized water using an atmospheric‐pressure radical source to investigate the role of neutral radicals in bactericidal efficacy radical‐activated (RAW). The RAW is investigated a colony‐forming unit assay with Escherichia coli . Both and reactive oxygen nitrogen species (RONS) concentration stored are over period month. E. was completely sterilized even for 7 days, indicating strong effect. However, deterioration 14 days confirmed, which does not have clear correlation...
Non-thermal atmospheric-pressure plasma (NTAPP) has been widely studied for clinical applications, e.g., disinfection, wound healing, cancer therapy, hemostasis, and bone regeneration. It is being revealed that the physical chemical actions of have enabled these applications. Based on our previous report regarding plasma-stimulated regeneration, this study focused Achilles tendon repair by NTAPP. This first to reveal exposure NTAPP can accelerate using a well-established injury rat model....
Using ambient mass spectrometry, positive and negative ions created in an atmospheric-pressure plasma jet have been detected for a variation of different traces gases (Ar, N2, O2) added to the flow, downstream main helium discharge plasma. We find that such additions can change chemistry outflow plume. For instance, small amounts O2 increases formation ion clusters, e.g., water clusters H+(H2O)n (with n up 5) through hydration reactions, but decreases intensity heavy detected. With addition...