Functional interactions between different brain regions require connecting fiber tracts, the structural basis of human connectome. To assemble a comprehensive understanding neural network elements from microscopic to macroscopic dimensions, multimodal and multiscale approach has be envisaged. However, integration results complementary neuroimaging techniques poses particular challenge. In this paper, we describe steadily evolving technique referred as three-dimensional polarized light...

Research of the human brain connectome requires multiscale approaches derived from independent imaging methods ideally applied to same object. Hence, comprehensible strategies for data integration across modalities and scales are essential. We have successfully established a concept bridge spatial microscopic fiber orientation measurements based on 3D-Polarized Light Imaging (3D-PLI) meso- or macroscopic dimensions. By creating distribution functions (pliODFs) high-resolution vector via...

Abstract Polarized light imaging (PLI) is a method to image fiber orientation in gross histological brain sections based on the birefringent properties of myelin sheaths. The uses transmission polarized quantitatively estimate and inclination angles at every point imaged section. Multiple can be assembled into 3D volume, from which extent tracts extracted. This article describes physical principles PLI two major applications method: white matter rat generation maps human gray matter....

Although the primate visual system has been extensively studied, detailed spatial organization of white matter fiber tracts carrying information between areas not fully established. This is mainly due to large gap tracer studies and diffusion-weighted MRI studies, which focus on specific axonal connections macroscale tracts, respectively. Here we used 3D polarization light imaging (3D-PLI), enables direct visualization at micrometer resolution, identify visualize system, such as stratum...

For developing a detailed network model of the brain based on image reconstructions, it is necessary to spatially resolve crossing nerve fibers. The accuracy hereby depends many factors, including spatial resolution imaging technique. 3D Polarized Light Imaging (3D-PLI) allows three-dimensional reconstruction fiber tracts in whole sections with micrometer in-plane resolution, but leaves uncertainties pixels containing Here we introduce Scattered (SLI) substructure crossings. measurement...

Polarised light imaging (PLI) utilises the birefringence of myelin sheaths in order to visualise orientation nerve fibres microtome sections adult human post-mortem brains at ultra-high spatial resolution. The preparation for PLI involves fixation, freezing and cutting into 100-mum-thick sections. Hence, geometrical distortions histological are inevitable have be removed 3D reconstruction subsequent fibre tracking. We here present a processing pipeline these using derived multimodal images...

Disentangling human brain connectivity requires an accurate description of nerve fiber trajectories, unveiled via detailed mapping axonal orientations. However, this is challenging because axons can cross one another on a micrometer scale. Diffusion magnetic resonance imaging (dMRI) be used to infer it sensitive alignment, but has limited spatial resolution and specificity. Scattered light (SLI) small-angle X-ray scattering (SAXS) reveal orientations with microscopic high specificity,...

The method 3D polarised light imaging (3D-PLI) measures the birefringence of histological brain sections to determine spatial course nerve fibres (myelinated axons). While in-plane fibre directions can be determined with high accuracy, computation out-of-plane inclinations is more challenging because they are derived from strength signals (retardation), which depends e.g. on amount fibres. One possibility improve accuracy consider average transmitted intensity (transmittance weighting)....

The correct reconstruction of individual (crossing) nerve fibers is a prerequisite when constructing detailed network model the brain. recently developed technique Scattered Light Imaging (SLI) allows crossing fiber pathways in whole brain tissue samples with micrometer resolution: orientations are determined by illuminating unstained histological sections from different directions, measuring transmitted scattered light under normal incidence, and studying intensity profiles each pixel...

Abstract The sagittal stratum is a prominent and macroscopically clearly visible white-matter structure within occipital parietal lobes with highly organized of parallel fibers running in rostro-caudal direction. Apart from the major tract through, i.e., optic radiation, source arrangement other only partially understood. Recent diffusion imaging studies in-vivo suggest additional minor fiber directions, perpendicular to ones, but spatial resolution does not allow resolve them, unambiguously...

3D-Polarized Light Imaging (3D-PLI) is a unique technique that enables high-resolution three-dimensional mapping of the nerve fiber architecture in unstained histological sections human brain. 3D-PLI based on detection intrinsic tissue birefringence caused by fibers. The measured birefringent signals comprise entangled information both spatial orientation and local density. In this study, we introduce novel approach to effectively unambiguously unravel interrelation, for providing reliable...

Abstract The structural connectivity of the brain has been addressed by various imaging techniques such as diffusion weighted magnetic resonance (DWMRI) or specific microscopic approaches based on histological staining label-free using polarized light (e.g., three-dimensional Polarized Light Imaging (3D-PLI), Optical Coherence Tomography (OCT)). These methods are sensitive to different properties fiber enwrapping myelin sheaths i.e. distribution basic protein (histology), apparent...

We introduce a new approach for the elastic registration of high-resolution 3D polarized light imaging (3D PLI) data histological sections human brain. For accurate different types PLI modalities, we propose novel intensity similarity measure that is based on least-squares formulation normalized cross-correlation. Moreover, present fully automatic pipeline rigid and images with blockface reference including preprocessing step. have successfully evaluated our using manually obtained ground...

The neuroimaging technique 3D-polarized light imaging (3D-PLI) has opened up new avenues to study the complex nerve fiber architecture of human brain at sub-millimeter spatial resolution. This polarimetry is applicable histological sections postmortem brains utilizing birefringence fibers caused by regular arrangement lipids and proteins in myelin sheaths surrounding axons. 3D-PLI provides a three-dimensional description anatomical wiring scheme defined in-section direction angle...

ABSTRACT BACKGROUND AND PURPOSE To examine the possible effects of intravenous thrombolysis on time course apparent diffusion coefficient in patients with acute middle cerebral artery infarct. METHODS Serial MRI data all 190 MR examinations including diffusion‐weighted imaging (DWI), map (ADC map) and T 2 ‐weighted (T w) 74 initial (study group; N = 37) or conservative stroke treatment (control were retrospectively analyzed. A trend function was fitted to relative values (rADC, rDWI, rT...

A neuroanatomic method to visualize and extract the main nerve fiber orientation in histological sections of human brain is presented. The so-called Polarized Light Imaging (PLI) enables high resolution large-scale studies white matter architecture utilizing its birefringence properties. can basically be attributed regular arrangement lipids myelin sheaths that enclose fibers. Consequently, linearly polarized light becomes elliptically when traveling through sections. final state...