A5022945512- Advanced Neuroimaging Techniques and Applications
- Advanced MRI Techniques and Applications
- Functional Brain Connectivity Studies
- Optical Imaging and Spectroscopy Techniques
- Medical Image Segmentation Techniques
- Cell Image Analysis Techniques
- Advanced Fluorescence Microscopy Techniques
- Optical Polarization and Ellipsometry
- Neurogenesis and neuroplasticity mechanisms
- Particle physics theoretical and experimental studies
- Morphological variations and asymmetry
- Neuroscience and Neuropharmacology Research
- Particle Detector Development and Performance
- Blind Source Separation Techniques
- Neural dynamics and brain function
- EEG and Brain-Computer Interfaces
- Memory and Neural Mechanisms
- Optical Coherence Tomography Applications
- Spaceflight effects on biology
- Advanced Vision and Imaging
- Spectroscopy Techniques in Biomedical and Chemical Research
- Medical Imaging Techniques and Applications
- Photoreceptor and optogenetics research
- Brain Tumor Detection and Classification
- Spectroscopy and Chemometric Analyses
Forschungszentrum Jülich
2015-2024
University of Wuppertal
2010-2024
Montreal Neurological Institute and Hospital
2023
McGill University
2023
Western University
2023
Ernst Ruska Centre
2023
Heinrich Heine University Düsseldorf
2015-2023
Düsseldorf University Hospital
2023
Athinoula A. Martinos Center for Biomedical Imaging
2019
Charité - Universitätsmedizin Berlin
2019
Basic principles of bird and mammal brains Mammals can be very smart. They also have a brain with cortex. It has thus often been assumed that the advanced cognitive skills mammals are closely related to evolution cerebral However, birds smart, several species show amazing abilities. Although lack cortex, they do pallium, this is considered analogous, if not homologous, An outstanding feature mammalian cortex its layered architecture. In detailed anatomical study Stacho et al. describe...
Functional interactions between different brain regions require connecting fiber tracts, the structural basis of human connectome. To assemble a comprehensive understanding neural network elements from microscopic to macroscopic dimensions, multimodal and multiscale approach has be envisaged. However, integration results complementary neuroimaging techniques poses particular challenge. In this paper, we describe steadily evolving technique referred as three-dimensional polarized light...
While hippocampal connectivity is essential to normal memory function, our knowledge of human circuitry largely inferred from animal studies. Using polarized light microscopy at 1.3 µm resolution, we have directly visualized the 3D course key medial temporal pathways in 3 ex vivo hemispheres and 2 vervet monkey hemispheres. The multiple components perforant path system were clearly identified: Superficial sheets fibers emanating entorhinal cortex project presubiculum parasubiculum,...
Abstract In recent years, brain research has indisputably entered a new epoch, driven by substantial methodological advances and digitally enabled data integration modelling at multiple scales—from molecules to the whole brain. Major are emerging intersection of neuroscience with technology computing. This science combines high-quality research, across scales, culture multidisciplinary large-scale collaboration, translation into applications. As pioneered in Europe’s Human Brain Project...
Research of the human brain connectome requires multiscale approaches derived from independent imaging methods ideally applied to same object. Hence, comprehensible strategies for data integration across modalities and scales are essential. We have successfully established a concept bridge spatial microscopic fiber orientation measurements based on 3D-Polarized Light Imaging (3D-PLI) meso- or macroscopic dimensions. By creating distribution functions (pliODFs) high-resolution vector via...
The human hippocampus plays a key role in memory management and is one of the first structures affected by Alzheimer's disease. Ultra-high magnetic resonance imaging provides access to its inner structure vivo. However, gradient limitations on clinical systems hinder connectivity microstructure. A major target this paper demonstration diffusion MRI potential, using ultra-high field (11.7 T) strong gradients (750 mT/m), reveal extra- intra-hippocampal addition To purpose, multiple-shell...
The neuroimaging technique three-dimensional polarized light imaging (3D-PLI) provides a high-resolution reconstruction of nerve fibres in human post-mortem brains. orientations the are derived from birefringence measurements histological brain sections assuming that fibres—consisting an axon and surrounding myelin sheath—are uniaxial birefringent measured optic axis is oriented direction (macroscopic model). Although experimental studies support this assumption, molecular structure sheath...
The avian brain displays a different architecture compared to mammals. This has led the first pioneers of comparative neuroanatomy wrong conclusions about bird evolution by assuming that telencephalon is hypertrophied striatum. Based on growing evidence from divers analysis demonstrating most forebrain pallial in nature, this view substantially changed during past decades. Further, birds show cognitive abilities comparable or even exceeding those some mammals, without "six-layered" cortex....
A GPU-based tool to generate realistic phantoms of the brain microstructure is presented. Using a spherical meshing technique which decomposes each microstructural item into set overlapping spheres, phantom construction made very fast while reliably avoiding collisions between items in scene. This novel method applied human white matter components, namely axonal fibers, oligodendrocytes and astrocytes. The algorithm reaches high values packing density angular dispersion for even case...
Abstract When transmitting polarised light through histological brain sections, different types of diattenuation (polarisation-dependent attenuation light) can be observed: In some regions, the is minimally attenuated when it parallel to nerve fibres (referred as D + ), in others, maximally − ). The underlying mechanisms these effects and their relationship tissue properties were so far unknown. Here, we demonstrate experimental studies that both observed samples from species (rodent,...
Classic anatomical atlases depict a contralateral hemispheral representation of each side the face. Recently, however, bilateral projection hemiface was hypothesized, based on animal studies that showed coexistence an additional trigeminothalamic tract sprouting from trigeminal principal sensory nucleus ascends ipsilaterally. This study aims to provide substrate for hypothesized projection. Three post-mortem human brainstems were scanned and diffusion magnetic resonance imaging at 11.7T. The...
Unraveling the structure and function of brain requires a detailed knowledge about neuronal connections, i.e., spatial architecture nerve fibers. One most powerful histological methods to reconstruct three-dimensional fiber pathways is 3D-polarized light imaging (3D-PLI). The technique measures birefringence sections derives orientations whole human with micrometer resolution. However, yields only single orientation for each measured tissue voxel even if it composed fibers different...
Although the primate visual system has been extensively studied, detailed spatial organization of white matter fiber tracts carrying information between areas not fully established. This is mainly due to large gap tracer studies and diffusion-weighted MRI studies, which focus on specific axonal connections macroscale tracts, respectively. Here we used 3D polarization light imaging (3D-PLI), enables direct visualization at micrometer resolution, identify visualize system, such as stratum...
Analyzing the structure of neuronal fibers with single axon resolution in large volumes is a challenge connectomics. Different technologies try to address this goal; however, they are limited either by ineffective labeling or achievable resolution. The possibility discriminating between different adjacent myelinated axons gives opportunity providing more information about fiber composition and architecture within specific area. Here, we propose MAGIC (Myelin Autofluorescence imaging Glycerol...
For developing a detailed network model of the brain based on image reconstructions, it is necessary to spatially resolve crossing nerve fibers. The accuracy hereby depends many factors, including spatial resolution imaging technique. 3D Polarized Light Imaging (3D-PLI) allows three-dimensional reconstruction fiber tracts in whole sections with micrometer in-plane resolution, but leaves uncertainties pixels containing Here we introduce Scattered (SLI) substructure crossings. measurement...
Polarised light imaging (PLI) utilises the birefringence of myelin sheaths in order to visualise orientation nerve fibres microtome sections adult human post-mortem brains at ultra-high spatial resolution. The preparation for PLI involves fixation, freezing and cutting into 100-mum-thick sections. Hence, geometrical distortions histological are inevitable have be removed 3D reconstruction subsequent fibre tracking. We here present a processing pipeline these using derived multimodal images...
White matter is composed of irregularly packed axons leading to a structural disorder in the extra-axonal space. Diffusion MRI experiments using oscillating gradient spin echo sequences have shown that diffusivity transverse this space dependent on frequency employed sequence. In study, we observe same frequency-dependence 3D simulations diffusion process disordered media. We design novel white numerical phantom generation algorithm which constructs biomimicking geometric configurations with...
High-resolution multiscale and multimodal 3D models of the brain are essential tools to understand its complex structural functional organization. Neuroimaging techniques addressing different aspects organization should be integrated in a reference space enable topographically correct alignment subsequent analysis various datasets their modalities. The Waxholm Space (http://software.incf.org/software/waxholm-space) is publicly available coordinate-based standard for mapping registration...
The hippocampus is an archicortical structure, consisting of subfields with unique circuits. Understanding its microstructure, as proxied by these subfields, can improve our mechanistic understanding learning and memory has clinical potential for several neurological disorders. One prominent issue how to parcellate, register, or retrieve homologous points between two hippocampi grossly different morphologies. Here, we present a surface-based registration method that solves this in...