A5091592836- RNA modifications and cancer
- Cancer-related gene regulation
- RNA and protein synthesis mechanisms
- Cancer-related molecular mechanisms research
- Circular RNAs in diseases
- RNA Research and Splicing
- Genetics and Neurodevelopmental Disorders
- MicroRNA in disease regulation
- Genetic Syndromes and Imprinting
- Kruppel-like factors research
- Epigenetics and DNA Methylation
University of Copenhagen
2011-2023
Roche (Denmark)
2022
Ribose methylation is one of the two most abundant modifications in human ribosomal RNA and believed to be important for ribosome biogenesis, mRNA selectivity translational fidelity. We have applied RiboMeth-seq rRNA from HeLa cells ribosome-wide, quantitative mapping 2′-O-Me sites obtained a comprehensive set 106 sites, including novel with plausible box C/D guide RNAs assigned all but three sites. find approximately two-thirds fully methylated remainder fractionally modified support...
The past decade has shown mammalian genomes to be pervasively transcribed and identified thousands of noncoding (nc) transcripts. It is currently unclear what extent these transcripts are functional importance, as experimental evidence exists for only a small fraction. Here, we characterize the expression evolutionary conservation properties 12,115 known novel nc transcripts, including structural RNAs, long RNAs (lncRNAs), antisense EvoFold predictions, ultraconserved elements, expressed...
miRNAs are small regulatory RNAs that, due to their considerable potential target a wide range of mRNAs, implicated in essentially all biological process, including cancer. miR-10a is particularly interesting considering its conserved location the Hox cluster developmental regulators. A role for this microRNA has been described regulation as well various cancers. However, previous studies exclusively based on transient knockdowns miRNA and extensively study loss we have generated knock out...
Abstract Angelman syndrome (AS) is a severe neurodevelopmental disorder caused by the loss of neuronal E3 ligase UBE3A with no available treatment. Restoring levels via downregulation paternally cis-acting long non-coding antisense transcript (UBE3A-ATS) potential disease modifying. Developing molecules targeting human UBE3A-ATS challenging because it expressed only in neurons and lacks animal species sequence conservation. To overcome this, we performed library screen locked-nucleic acid...
Abstract Translational regulation impacts both pluripotency maintenance and cell differentiation. To what degree the ribosome itself exerts control over this process remains unanswered. Accumulating evidence has demonstrated heterogeneity in composition various organisms. 2’-O-methylation of rRNA represents an important source heterogeneity, where site-specific alteration methylation levels can modulate translation. Here we explore changes during mouse brain development tri-lineage...
Abstract Ribosomes are complex ribozymes that interpret genetic information by translating messenger RNA (mRNA) into proteins. Natural variation in ribosome composition has been documented several organisms, and can stem from different sources. A key question is whether specific control over heterogeneity represents a mechanism which translation be regulated. We used RiboMeth-seq to demonstrate differential 2’-O-methylation of ribosomal (rRNA) considerable source human cells, modification...