A heterotrimeric G alpha i subunit, i-3, is localized on Golgi membranes in LLC-PK1 and NRK epithelial cells where it colocalizes with mannosidase II by immunofluorescence. The i-3 was found to be the cytoplasmic face of cisternae distributed across whole stack. subunit isolated rat liver Western blotting apparatus ADP ribosylated pertussis toxin. were stably transfected an MT-1, inducible promoter order overexpress membranes. intracellular processing constitutive secretion basement membrane...

Research has found a strong inverse association between discrimination and health well-being. Most of these studies have been conducted among African-Americans, examined the relationship at individual-level. To fill gaps in knowledge we estimated prevalence perceived nationally representative sample Latino adults US, investigated state-level anti-immigrant policies discrimination. We merged survey data with policy index. First, fit hierarchical logistic regression models to test crude...

The effect of brefeldin-A (BFA), a reversible inhibitor vesicular transport, on cholera toxin (CT)-induced Cl- secretion (Isc) was examined in the polarized human intestinal cell line, T84. Pretreatment T84 monolayers with 5 microM BFA reversibly inhibited Isc response to apical or basolateral addition 120 nM CT (2.4 +/- 0.5 vs. 68 3 microA/cm2, n = 5). In contrast, did not inhibit responses cAMP agonist VIP (63 7 microA/cm2). had no surface binding and endocytosis functional fluorescent...

To ameliorate the public health problem of adolescent suicide, it is imperative to go beyond simply establishing which factors increase risk. Multiple often interact in such a way that escalates suicide risk, and some combinations may be particularly perilous. Therefore, essential examine sequence interplay these various contributors understand how they confer enhance our understanding this process, we used qualitative in-depth interviews with 20 psychiatrically hospitalized adolescents who...

We have recently demonstrated that the amiloride-sensitive Na+ channel in apical membrane of renal epithelial cell line, A6, is modulated by alpha i-3 subunit Gi-3 protein. also showed a 700-kDa protein complex can be purified from membranes A6 epithelia which (a) reconstitute influx liposomes and planar bilayer (b) consists six major bands observed on reducing sodium dodecyl sulfate-polyacrylamide gels with molecular masses ranging 35 to 320 kDa. The present study was undertaken determine...

This randomized controlled trial (Clinicaltrials.gov NCT [01996280]) compared the efficacy of a brief motivational interview (MI) adapted to address social stressors and cultural influences (culturally MI [CAMI]) standard for heavy-drinking Latinxs. CAMI was hypothesized reduce heavy drinking days frequency alcohol-related consequences more than MI. Moderators treatment effect were explored.

Basement membrane proteins such as the heparan sulfate proteoglycan (HSPG) are secreted in a polarized fashion from basolateral of epithelial cells. We have used microtubule-disrupting drug colchicine to study role microtubule network directing constitutive secretion LLC-PK1 renal Microtubule depolymerization induced by resulted fragmentation and redistribution fluorescently labeled trans-Golgi membranes. Increased immunofluorescent staining HSPG was associated with these dispersed Golgi...

Most master of social work (MSW) programs provide minimal education or training on suicide prevention, intervention, and postvention despite that the majority workers encounter suicidal clients during their professional careers. This study describes development, implementation, evaluation a course in an MSW program. Changes student suicide-related knowledge, confidence, preparedness were evaluated using pre/post design. We found statistically significant increases knowledge (t [df = 21]...

Heterotrimeric guanine nucleotide-regulatory (G) proteins are associated with a variety of intracellular membranes and specific plasma membrane domains. In polarized epithelial LLC-PK1 cells we have shown previously that endogenous G alpha i-2 is localized on the basolateral membrane, whereas i-3 Golgi membranes. The targeting these highly homologous i to distinct domains was studied by transfection expression chimeric in cells. Chimeric cDNAs were constructed from for introduced into pMXX...

Researchers have found an inverse relationship between immigrant status and violence perpetration. Most studies examined Mexican immigrants, few assessed immigration factors other than nativity. Additionally, the majority focused on most serious forms of despite fact that moderate is more common. Using data from 2008 Boston Youth Survey, we generated prevalence estimates peer perpetration across related factors, whether risk for differed by these variables, explored contribution to Recent...

There is an increased interest in how neighborhood social processes, such as collective efficacy, may protect mental health. Yet little known about stable these processes are over time, or to change them influence other downstream factors. We used a population-based, repeat cross-sectional study of adults (n=5135) assess stability efficacy for families 38 Boston neighborhoods across 4 years (2006, 2008, 2010) (the Neighborhood Survey). test temporal and within using 2-level random effects...

ABSTRACT The formation of vesicles for protein trafficking requires the dynamic binding cytosolic coat proteins onto Golgi membranes and this is regulated by a variety GTPases, including heterotrimeric G pro-teins. We have previously shown presence pertussis toxin-sensitive i-3 on demonstrated functional role in secretory through complex. also described brefeldin A-sensitive phosphoprotein, p200, which found cytoplasm membranes. present study investigates regulation p200 to An vitro assay...

The prevalence and immigration‐related correlates of deliberate self‐injury (DSI) suicidal ideation (SI) were estimated in a sample Boston public high school students 2006. Compared with U.S.‐born youth, immigrant youth not at increased risk for DSI or SI, even if they had experienced discrimination due to their ancestry. By contrast, who reported having been discriminated against because ancestry an (odds ratio [OR] = 3.1, 95% confidence interval [CI] 1.6–5.9) (OR 2.1, CI 1.2–3.8)....

Journal Article Social Work's Ethical Responsibility to Train MSW Students Work with Suicidal Clients Get access Joanna Almeida, Almeida 1Joanna ScD, MPH, MSW, and Kimberly H. McManama O'Brien, PhD, LCSW, are assistant professors, School of Work, Simmons College, Boston. Ken Norton, LICSW, ACSW, is executive director, New Hampshire Chapter, National Alliance on Mental Illness, Concord, NH. *Address correspondence 300 the Fenway, Office P412-B, Boston, MA 02115; e-mail:...

Racial/ethnic minority and foreign-born women in the United States are at high risk of experiencing racial discrimination, which is associated with adverse health outcomes. Although discrimination metabolic disturbances such as insulin resistance type 2 diabetes, more studies should examine its effect on gestational diabetes mellitus (GDM), highest among racial/ethnic women.We used New York City Pregnancy Risk Assessment Monitoring System survey data (2012-2014) linked birth certificate...