A5091741220- Plant tissue culture and regeneration
- Banana Cultivation and Research
- Plant-Microbe Interactions and Immunity
- CRISPR and Genetic Engineering
- Antioxidant Activity and Oxidative Stress
- Animal Genetics and Reproduction
- Crop Yield and Soil Fertility
- Plant Growth Enhancement Techniques
- Phytase and its Applications
- Cassava research and cyanide
- Plant Pathogenic Bacteria Studies
- Plant Micronutrient Interactions and Effects
- Agricultural Science and Fertilization
- Plant Reproductive Biology
- Transgenic Plants and Applications
- Nematode management and characterization studies
Queensland University of Technology
2011-2022
Banana (Musa spp.) is a staple food for more than 400 million people. Over 40% of world production and virtually all the export trade based on Cavendish banana. However, banana under threat from virulent fungus, Fusarium oxysporum f. sp. cubense tropical race 4 (TR4) which no acceptable resistant replacement has been identified. Here we report identification transgenic with resistance to TR4. In our 3-year field trial, two lines Cavendish, one transformed RGA2, gene isolated TR4-resistant...
Vitamin A deficiency remains one of the world's major public health problems despite food fortification and supplements strategies. Biofortification staple crops with enhanced levels pro-vitamin (PVA) offers a sustainable alternative strategy to both supplementation. As proof concept, PVA-biofortified transgenic Cavendish bananas were generated field trialed in Australia aim achieving target level 20 μg/g dry weight (dw) β-carotene equivalent (β-CE) fruit. Expression Fe'i banana-derived...
Summary Fusarium wilt, caused by oxysporum f. sp. cubense (Foc), is one of the most devastating diseases banana ( Musa spp.). Apart from resistant cultivars, there are no effective control measures for disease. We investigated whether transgenic expression apoptosis‐inhibition‐related genes in could be used to confer disease resistance. Embryogenic cell suspensions cultivar, ‘Lady Finger’, were stably transformed with animal that negatively regulate apoptosis, namely Bcl‐xL , Ced‐9 and Bcl‐2...
Bananas are one of the top ten world food crops. Unlike most other major crops, bananas difficult to genetically improve. The challenge is that nearly all banana cultivars and landraces triploids, with high levels male female infertility. There a number international conventional breeding programs many these developing new cultivars. However, it virtually impossible backcross bananas, thus excluding possibility introgressing traits into current cultivar. alternative strategy “modify”...
The death of plant cells in culture following exposure to Agrobacterium tumefaciens remains a major obstacle developing Agrobacterium-mediated transformation into highly efficient genotype-independent technology. Here, we present evidence that A. induces cell banana suspensions. More than 90% embryogenic died after and was accompanied by subset features associated with apoptosis mammalian cells, including DNA laddering, fragmentation, formation apoptotic-like bodies. Importantly, these...
Abstract Genetic improvement of commercially accepted banana cultivars is strongly reliant on the ability to introduce genes that encode important agro-traits such as disease resistance. In most cases this can only be achieved using a transgenic approach. Public and regulatory acceptance these events would greatly increase with “clean” single copy integration free selectable marker gene extraneous vector backbone. This also allow for successive addition new traits they become available....
Bananas and plantains (Musa spp.) are an important staple food security crop in sub-Saharan Africa. In Uganda, where the consumption of East African highland banana (EAHB) is highest world, population suffers from a high incidence vitamin A deficiency (VAD). Since pro-vitamin carotenoids (pVAC) made available through can help alleviate these ailments, we set out to identify most suitable variety use future biofortification strategies genetic engineering. The study focussed on eight popular...
Genetic modification is one possible strategy to generate bananas (Musa spp.) with resistance the soil-borne pathogen causing Fusarium wilt. The availability of banana root-specific promoters target transgene expression sites infection would be beneficial. We have assessed 18 promoter sequences derived from a range plant species for their profiles in tissues identify those activity. Promoter were isolated and fused β-glucuronidase (GUS) gene assess levels tissue specificity both model...
An experiment was conducted in the Horticultural Farm of Sher-e-Bangla Agricultural University, Nagar, Dhaka-1207 during period from October 2010 to March 2011 find out effect GA 3 and seedling age on growth yield tomato.The laid randomized complete block design (RCBD) with three replications.The consisted four concentration such as control G 0 = no , 1 75 ppm 2 100 =125 ; different ages S 20 days, 25 days 30 old seedling.All parameter varied significantly at DAT.The combined (125 seedling)...