A5056830004- Plant Virus Research Studies
- Plant tissue culture and regeneration
- Plant and Fungal Interactions Research
- Transgenic Plants and Applications
- Plant Pathogenic Bacteria Studies
- CRISPR and Genetic Engineering
- Banana Cultivation and Research
- Plant Disease Resistance and Genetics
- Insect-Plant Interactions and Control
- Mosquito-borne diseases and control
- Sugarcane Cultivation and Processing
- Plant Pathogens and Fungal Diseases
- Phytoplasmas and Hemiptera pathogens
- Cocoa and Sweet Potato Agronomy
- Bacteriophages and microbial interactions
- Viral gastroenteritis research and epidemiology
- Viral Infections and Vectors
- Vector-Borne Animal Diseases
- Plant-Microbe Interactions and Immunity
- Chromosomal and Genetic Variations
- Plant Disease Management Techniques
- Viral Infections and Immunology Research
- Pacific and Southeast Asian Studies
- Insect Resistance and Genetics
- Herpesvirus Infections and Treatments
Queensland University of Technology
2014-2023
Renaissance Services (United States)
2023
University of Cambridge
2017
Institute of Archaeology
2004
The University of Queensland
1991-1993
Banana (Musa spp.) is a staple food for more than 400 million people. Over 40% of world production and virtually all the export trade based on Cavendish banana. However, banana under threat from virulent fungus, Fusarium oxysporum f. sp. cubense tropical race 4 (TR4) which no acceptable resistant replacement has been identified. Here we report identification transgenic with resistance to TR4. In our 3-year field trial, two lines Cavendish, one transformed RGA2, gene isolated TR4-resistant...
Agroinfiltration is a simple and effective method of delivering transgenes into plant cells for the rapid production recombinant proteins has become preferred transient expression platform to manufacture biologics in plants. Despite its popularity, few studies have sought improve efficiency agroinfiltration further increase protein yields. This study aimed agroinfiltration-based gene Nicotiana benthamiana by improving all levels transgenesis. Using benchmark pEAQ-HT deconstructed virus...
Vitamin A deficiency remains one of the world's major public health problems despite food fortification and supplements strategies. Biofortification staple crops with enhanced levels pro-vitamin (PVA) offers a sustainable alternative strategy to both supplementation. As proof concept, PVA-biofortified transgenic Cavendish bananas were generated field trialed in Australia aim achieving target level 20 μg/g dry weight (dw) β-carotene equivalent (β-CE) fruit. Expression Fe'i banana-derived...
We have cloned, sequenced and analysed an additional five circular ssDNA components of banana bunchy top virus (BBTV) which we called 2, 3, 4, 5 6. These were present in all BBTV infections tested. Four these (components 6) had one large open reading frame (ORF) the virion sense located 3′ a stem-loop structure. Each ORF potential TATA box or two polyadenylation signals associated with it each signal GC-rich region containing trinucleotide sequence TTG. A number ORFs identified component 2...
Summary Fusarium wilt, caused by oxysporum f. sp. cubense (Foc), is one of the most devastating diseases banana ( Musa spp.). Apart from resistant cultivars, there are no effective control measures for disease. We investigated whether transgenic expression apoptosis‐inhibition‐related genes in could be used to confer disease resistance. Embryogenic cell suspensions cultivar, ‘Lady Finger’, were stably transformed with animal that negatively regulate apoptosis, namely Bcl‐xL , Ced‐9 and Bcl‐2...
Sixteen viruses, belonging to 16 species of begomovirus, that infect crops and weeds in Vietnam were identified. Sequence analysis the complete genomes showed nine viruses (six monopartite three bipartite) belong novel five them identified for first time. Additionally, eight DNA- β nanovirus-like DNA-1 molecules also found associated with some viruses. Five novel. Importantly, a second bipartite Corchorus golden mosaic virus, shared several features previously characterized virus yellow vein...
One DNA component of the banana bunchy top virus (BBTV) genome was cloned and sequenced. This is present as a circular, ssDNA in virions consists 1111 nucleotides. It contains one large open reading frame (ORF) 858 nucleotides virion sense; this ORF encodes putative replicase based on presence dNTP-binding motif (GGEGKT). Two smaller ORFs (249 366 nucleotides), complementary orientation, could not be assigned any obvious function. Neither these had significant sequence homology with known...
Virus-like particles were purified from banana plants with bunchy top disease. These isometric a diameter of 18 to 20 nm and density 1.28 1.30 g/ml in caesium sulphate. Associated these an ssDNA about 1 kb one major protein M r 20100. DsDNA was synthesized nucleic acid extracts cloned. One clone, pBT338, hybridized specifically (i) sap infected virus (BBTV) but not healthy (ii) the small virus-like particles. Banana disease transmitted bananas by aphid inoculation it demonstrated that It is...
In this study, we describe a novel protein production platform that provides both activation and amplification of transgene expression in planta. The Plant Activation (INPACT) system is based on the replication machinery tobacco yellow dwarf mastrevirus (TYDV) essentially transient gene from stably transformed plant, thus combining advantages means expression. INPACT cassette uniquely arranged such interest split only reconstituted presence TYDV-encoded Rep/RepA proteins. placed under...
Banana bunchy top virus (BBTV) DNA component 1 from isolates 10 different countries was cloned and sequenced the sequences were aligned compared. This analysis indicated two groups: South Pacific group (isolates Australia, Burundi, Egypt, Fiji, India, Tonga Western Samoa) Asian Philippines, Taiwan Vietnam). The mean sequence difference within each 1.9 to 3.0% between groups approximately 10%, but some parts of differed more than others. However, protein encoded by major open reading frame,...
Banana plants are hosts to a large number of streak virus (BSV) species. However, diagnostic methods for BSV inadequate because the considerable genetic and serological diversity among isolates presence integrated sequences in some banana cultivars which leads false positives. In this study, sequence-nonspecific, rolling-circle amplification (RCA) technique was developed shown overcome these limitations detection subsequent characterization infecting banana. This discriminate between...
Somatic embryogenesis and transformation systems are indispensable modern plant breeding components since they provide an alternative platform to develop control strategies against the plethora of pests diseases affecting many agronomic crops. This review discusses some factors somatic transformation, highlights advantages limitations these explores as tools for development crops with improved traits. The regeneration non-chimeric transgenic through introduced disease pest-resistant genes...
Bananas are one of the top ten world food crops. Unlike most other major crops, bananas difficult to genetically improve. The challenge is that nearly all banana cultivars and landraces triploids, with high levels male female infertility. There a number international conventional breeding programs many these developing new cultivars. However, it virtually impossible backcross bananas, thus excluding possibility introgressing traits into current cultivar. alternative strategy “modify”...
Although cowpea is an important food security crop in Africa, its production constrained by insect pests such as the legume pod borer (Maruca vitrata) (Lepidoptera). Potential control strategies have focussed on using insecticidal toxins crystal Cry proteins and vegetative (Vips) encoded cry vip genes, respectively, of bacterium Bacillus thuringiensis (Bt). This study sought to identify genes encoding active against Maruca Pod Borer (MPB), from Australian Bt isolates. A collection 224...
The major open reading frame of banana bunchy top virus (BBTV) DNA-1 encodes a putative replication initiation protein (Rep). In vitro, fusion BBTV Rep linked to maltose-binding exhibited both site-specific nicking and joining activities. These activities were dependent on the presence Mg2 or Mn2 , but did not require ATP. specifically cleaved ssDNA between bases 7 8 conserved nonanucleotide loop sequence which is present in virion-strand stem-loop common region each component. During this...
Green fluorescent protein (GFP)-tagging was used to determine the intracellular localization pattern of proteins encoded by banana bunchy top virus (BBTV) DNA-3, -4 and -6. The BBTV DNA-4, which possesses a hydrophobic N terminus, found localize exclusively cell periphery while DNA-3 -6 were in both nucleus cytoplasm. Co-expression DNA-4 revealed that able re-locate DNA-6 protein, but not periphery. 29 amino acid N-terminal region gene product appeared be essential for specific this since...
Banana leaf streak disease, caused by several species of banana virus (BSV), is widespread in East Africa. We surveyed for this disease Uganda and Kenya, used rolling-circle amplification to detect the presence BSV banana. Six distinct badnavirus sequences, three from were amplified which only partial sequences previously available. The complete genomes sequenced characterised. size organisation all six was characteristic other badnaviruses, including conserved functional domains present...