A5034281305- Single-cell and spatial transcriptomics
- Peroxisome Proliferator-Activated Receptors
- Nerve injury and regeneration
- Zebrafish Biomedical Research Applications
- Cell Image Analysis Techniques
- Neurogenesis and neuroplasticity mechanisms
- Pluripotent Stem Cells Research
- Cancer-related molecular mechanisms research
- 3D Printing in Biomedical Research
- Endoplasmic Reticulum Stress and Disease
- Cholesterol and Lipid Metabolism
- Adipose Tissue and Metabolism
- Cellular Mechanics and Interactions
- Congenital heart defects research
- MicroRNA in disease regulation
- Neuroscience and Neuropharmacology Research
- RNA Research and Splicing
- Image Processing Techniques and Applications
University of Helsinki
2017-2023
National Institute of Dental and Craniofacial Research
2020-2023
National Institutes of Health
2020-2023
Minerva Foundation
2019-2021
Abstract The ability of the pluripotent epiblast to contribute progeny all three germ layers is thought be lost after gastrulation. later-forming neural crest (NC) rises from ectoderm and it remains poorly understood how its exceptionally high stem-cell potential generate mesodermal- endodermal-like derivatives obtained. Here, we monitor transcriptional changes gastrulation neurulation using single-cell-Multiplex-Spatial-Transcriptomics (scMST) complemented with RNA-sequencing. We show...
Abstract Neurotrophins and their receptors have highly conserved evolutionary lineage in vertebrates including zebrafish. The NTRK2 receptor has two isoforms zebrafish, Ntrk2a Ntrk2b. spatio-temporal expression pattern of bdnf ntrk2b the zebrafish brain was studied using situ hybridization. robust corresponding to suggests that is key for brain, unlike its duplicate isoform ntrk2a . To study function, different genetic strategies, TILLING mutant morpholino oligonucleotides (MO), were used....
Abstract Lipid-induced toxicity is part of several human diseases, but the mechanisms involved are not fully understood. Fatty liver characterized by expression different growth and tissue factors. The neurotrophin, nerve factor (NGF) its pro-form, pro-NGF, present in fatty together with p75 neurotrophin receptor (p75NTR). Stimulation Huh7 hepatocyte cells NGF pro-NGF induced Sterol-regulator-element-binding protein-2 (SREBP2) activation increased Low-Density Lipoprotein Receptor (LDLR)...
The demand for single-cell level data is constantly increasing within life sciences. In order to meet this demand, robust cell segmentation methods that can tackle challenging in vivo tissues with complex morphology are required. However, currently available and volumetric analysis perform poorly on 3D images. Here, we generated ShapeMetrics, a MATLAB-based script segments cells and, by performing unbiased clustering using heatmap, separates the into subgroups according their morphological...
Peroxisome proliferator-activated receptor-γ coactivator-1α (PGC-1α) is a master regulator of mitochondria biogenesis and cell stress playing role in metabolic degenerative diseases. In the brain PGC-1α expression has been localized mainly to GABAergic interneurons but its overall not fully understood. We observed here that protein levels γ-aminobutyric acid (GABA) type A receptor-α2 subunit (GABARα2) were increased hippocampus cortex transgenic (Tg) mice overexpressing neurons. Along with...
The ability of the pluripotent epiblast to contribute progeny all three germ layers is thought be lost after gastrulation. later-forming neural crest (NC) rises from ectoderm and it remains poorly understood how its exceptionally high stem-cell potential generate mesodermal- endodermal-like cells obtained. We monitored transcriptional changes gastrulation neurulation using single-cell-Multiplex-Spatial-Transcriptomics (scMST) complemented with RNA-sequencing. Unexpectedly, we find...
Abstract Brain-derived neurotrophic factor (BDNF), together with its cognate receptor tyrosine kinase B (TrkB), plays an essential role in the development and plasticity of brain is widely implicated psychiatric diseases (Autry Monteggia, 2012). Due to highly conserved evolutionary lineage neurotrophins their receptors vertebrates, zebrafish a well-suited model for this study. The TrkB receptor, also known as NTRK2, has two forms zebrafish, Ntrk2a Ntrk2b. spatio-temporal expression pattern...