A5103008582- Microfluidic and Capillary Electrophoresis Applications
- 3D Printing in Biomedical Research
- Microfluidic and Bio-sensing Technologies
- Innovative Microfluidic and Catalytic Techniques Innovation
- Nanopore and Nanochannel Transport Studies
- Plant and soil sciences
- Nanofabrication and Lithography Techniques
- Anodic Oxide Films and Nanostructures
- Enhanced Oil Recovery Techniques
- Viral Infectious Diseases and Gene Expression in Insects
- Cancer Cells and Metastasis
- Agricultural and Food Production Studies
- Bacterial biofilms and quorum sensing
- Carbon Nanotubes in Composites
- Nanowire Synthesis and Applications
- Analog and Mixed-Signal Circuit Design
- Analytical Chemistry and Sensors
- Reservoir Engineering and Simulation Methods
- Advanced Chemical Sensor Technologies
- Advanced MEMS and NEMS Technologies
- Insect Resistance and Genetics
- Agriculture and Rural Development Research
- Latin American rural development
- Petroleum Processing and Analysis
- Cell Image Analysis Techniques
Florida International University
2020-2025
National Technological University
2014-2025
University of Buenos Aires
2009-2024
Pontificia Universidad Católica de Chile
2024
University of Miami
2023
Ministerio de Salud - Provincia de Buenos Aires
2022
Centro Científico Tecnológico - San Juan
2021
Universidad Nacional Arturo Jauretche
2021
Instituto Superior de Teologia Aplicada
2018
Consejo Nacional de Investigaciones Científicas y Técnicas
2018
Treatment of cancer patients with autologous T-cells expressing a chimeric antigen receptor (CAR) is one the most promising therapeutic modalities for hematological malignancy treatment. For this treatment, primary T-cell expansion needed. Microfluidic technologies can be used to better understand activation and proliferation. Microfluidics have had meaningful impact in way experimental biology biomedical research are approached general. Furthermore, microfluidic technology allows generation...
ABSTRACT This work describes a novel and cost‐effective method of polydimethylsiloxane (PDMS) microchips fabrication by using printing plate photopolymer called Flexcel as master mold (Fmold). has demonstrated the ability to generate multiple devices from single master, reaching minimum channel size 25 μm, structures height ranging 53 1500 μm achieving dimensions 1270 × 2062 mm 2 , which are larger than those obtained known techniques date. Scanning electron microscopy, atomic force...
Abstract A simple methodology to generate polydopamine (PDA) surfaces featured with color due thin‐film interference phenomena is presented. It based on depositing ultra‐thin films of a Si/Si 3 N 4 wafer that exhibits an interferential reflectance maximum right at the visible/UV boundary (∼400 nm). Therefore, small deposit PDA modifies optical path, in such manner wavelength red shifts. Because human eye very sensitive any change light spectral distribution visible region, film thickness...
This paper describes a methodology of photopolymer mold fabrication with multi-level microstructures for polydimethylsiloxane (PDMS) microfluidic device manufacture. Multi-level can be performed by varying UVA exposure time and channel width. Scanning Electron Microscopy (SEM), Atomic Force (AFM) profilometry techniques have been employed to characterize the molds. Multiple molds formed in unique piece. Overall height/depth structures reaches up 677 μm minimum 21 μm. The method provides...
Over the past few years, image analysis has emerged as a powerful tool for analyzing various cell biology parameters in an unprecedented and highly specific manner. The amount of data that is generated requires automated methods processing all resulting information. software available so far are suitable fluorescence phase contrast images, but often do not provide good results from transmission light microscopy due to intrinsic variation acquisition images technique itself (adjustment...
Conventional manufacturing methods for polydimethylsiloxane (PDMS)-based microdevices require multiple steps and elements that increase cost production time. Also, these PDMS are mostly limited to single use, it is difficult recover the contents inside microchannels or perform advanced microscopy visualization due their irreversible sealing method. Herein, we developed a novel method based on polymethylmethacrylate (PMMA) plates adjusted using mechanical pressure-based system. One...
Gene editing tools have triggered a revolutionary transformation in the realms of cellular and molecular physiology, serving as fundamental cornerstone for evolution disease models assays cell culture reactions, marked by various enhancements. Concurrently, microfluidics has emerged over recent decades versatile technology capable elevating performance reducing costs daily experiments across diverse scientific disciplines, with pronounced impact on biology. The amalgamation these...
Journal Article Production of monoclonal antibodies in microfluidic devices Get access Natalia Bourguignon, Bourguignon Facultad Regional Haedo, Universidad Tecnológica Nacional (UTN), Provincia de Buenos Aires CP 1706, Argentina Search for other works by this author on: Oxford Academic Google Scholar Carolina Attallah, Attallah Laboratorio Cultivos Celulares, Bioquímica y Ciencias Biológicas, del Litoral (UNL), CONICET, Santa Fe, 3000ZAA, Paola Karp, Karp Biotecnología Farmacéutica,...
Lab on a Chip (LOC) farming systems have emerged as powerful tool for single cell studies combined with non‐adherent culture substrate and capture chips the study of derived tumor spheres. Cancer is characterized by its cellular heterogeneity where only small population cancer stem cells (CSCs) are responsible metastases recurrences. Thus, in vitro strategy to formation cell‐derived sphere an attractive alternative identify CSCs. In this study, we test effectiveness microdevices analysis...
A method development aimed for high-throughput and automated antibody screening holds great potential areas ranging from fundamental molecular interactions to the discovery of novel disease markers, therapeutic targets, monoclonal engineering. Surface display techniques enable efficient manipulation large libraries in small volumes. Specifically, phage appeared as a powerful technology selecting peptides proteins with enhanced, target-specific binding affinities. Here, we present...
Microfluidic devices are a new platform for Enhanced Oil Recovery (EOR) assays. The application of Colloidad Dispersion Gels (CDG) injection method has been proposed in recent years. However, the mechanism oil recovery enhancement not completely elucidated. Its purpose is to mobilize by decreasing permeability channeled zones. success depends on characteristics water, porous medium and oil. Therefore, successful reservoir can be different another, hence importance methodology assessment...
This work shows the integration of a sensor based on carbon nanotubes using CMOS technology. A chip (CS) was designed and manufactured 0.30 μm process, leaving free window passivation layer that allowed deposition SWCNTs over electrodes. We successfully investigated with CS effect humidity temperature electrical transport properties SWCNTs. The possibility large scale process opens new route in design more efficient, low cost sensors high reproducibility their manufacture.
To produce innovative biopharmaceuticals, highly flexible, adaptable, robust, and affordable bioprocess platforms for bioreactors are essential. In this article, we describe the development of a large-area microfluidic bioreactor (LM bioreactor) mammalian cell culture that works at laminar flow perfusion conditions. The 184 cm2 32 cisterns LM is largest polydimethylsiloxane (PDMS) device fabricated by photopolymer flexographic master mold methodology, reaching final volume 2.8 mL. was...
Nowadays, image analysis has a relevant role in most scientific and research areas. This process is used to extract understand information from images obtain model, knowledge, rules the decision process. In case of biological areas, are acquired describe behavior agent time such as cells using mathematical computational approach generate system with automatic control. this paper, MCF7 model their growth death when they have been injected drug. These mammalian allow understanding behavior,...
Microfluidic tools have recently made possible many advances in biological and biomedical research. Research fields such as physics, engineering, chemistry biology combined to produce innovation microfluidics which has positively impacted diverse areas nucleotide sequencing, functional genomics, single-cell studies, single molecules assays diagnostics. Among these areas, regenerative medicine stem cells benefited from since had a profound impact on their applications. In this study, we...