A5042255048- Nanoparticle-Based Drug Delivery
- Alzheimer's disease research and treatments
- Gold and Silver Nanoparticles Synthesis and Applications
- 3D Printing in Biomedical Research
- Microfluidic and Bio-sensing Technologies
- Trace Elements in Health
- Nanoplatforms for cancer theranostics
- Photoacoustic and Ultrasonic Imaging
- Characterization and Applications of Magnetic Nanoparticles
- Genetic factors in colorectal cancer
- X-ray Spectroscopy and Fluorescence Analysis
- Quantum Dots Synthesis And Properties
- Genomic variations and chromosomal abnormalities
- Drug-Induced Adverse Reactions
- Graphene and Nanomaterials Applications
- Chemotherapy-related skin toxicity
- Health Promotion and Cardiovascular Prevention
- Intramuscular injections and effects
- Advanced Proteomics Techniques and Applications
- Advanced Photocatalysis Techniques
- Iron Metabolism and Disorders
- Phosphodiesterase function and regulation
- Neuroscience and Neuropharmacology Research
- Skin and Cellular Biology Research
- Supramolecular Self-Assembly in Materials
Italian Institute of Technology
2019-2023
National Technological University
2018-2020
Universidad Politécnica de Madrid
2011-2019
Consejo Nacional de Investigaciones Científicas y Técnicas
2018
University of Buenos Aires
2018
Research Institute Hospital 12 de Octubre
2015-2016
Universidad Autónoma de Madrid
2015
Colciencias
2014
Hospital General Universitario Gregorio Marañón
2013-2014
Abstract Nanoparticle‐based magnetic hyperthermia is a well‐known thermal therapy platform studied to treat solid tumors, but its use for monotherapy limited due incomplete tumor eradication at temperature (45 °C). It often combined with chemotherapy obtaining more effective therapeutic outcome. Cubic‐shaped cobalt ferrite nanoparticles (Co–Fe NCs) serve as agents and cytotoxic agent the known ion toxicity, allowing achievement of both heat effects from single platform. In addition this...
Cancer stem cells (CSCs) are the tumor cell subpopulation responsible for resistance to chemotherapy, recurrence, and metastasis. An efficient therapy must act on low proliferating quiescent-CSCs (q-CSCs). We here investigate effect of magnetic hyperthermia (MHT) in combination with local chemotherapy as a dual inhibit patient-derived colorectal qCR-CSCs. apply iron oxide nanocubes MHT heat mediators, coated thermoresponsive polymer (TR-Cubes) loaded DOXO (TR-DOXO) chemotherapeutic agent....
Lead-based halide perovskite nanocrystals are highly luminescent materials, but their sensitivity to humid environments and biotoxicity still important challenges solve. Here, we develop a stepwise approach encapsulate representative CsPbBr3 into water-soluble polymer capsules. We show that our protocol can be extended coated with different ligands, enabling an outstanding high photoluminescence quantum yield of ∼60% is preserved over two years in capsules dispersed water. demonstrate this...
The phosphodiesterase (PDE) 7 inhibitor S14 is a cell-permeable small heterocyclic molecule that able to cross the blood-brain barrier. We previously found intraperitoneal treatment with exerted neuroprotection in an Alzheimer's disease (AD) model (in APP/PS1 mice). objective of this study was investigate neurogenic and cellular effects oral administration on amyloid β (Aβ) overload.We orally administered PDE7 (15 mg/kg/day) or vehicle 6-month-old mice. After 5 weeks treatment, we evaluated...
Early diagnosis in Alzheimer's disease (AD), prior to the appearance of marked clinical symptoms, is critical prevent irreversible neuronal damage and neural malfunction that lead dementia death. Therefore, there an urgent need generate new contrast agents which reveal by a noninvasive method presence some pathological signs AD. In present study, we demonstrate for first time nanoconjugate composed magnetic nanoparticles bound antiferritin antibody, has been developed based on existence iron...
Here, we report a nanoplatform based on iron oxide nanocubes (IONCs) coated with bioresorbable polymer that, upon exposure to lytic enzymes, can be disassembled increasing the heat performances in comparison initial clusters. We have developed two-dimensional (2D) clusters by exploiting benchmark IONCs as mediators for magnetic hyperthermia and polyhydroxyalkanoate (PHA) copolymer, biodegradable produced bacteria that digested intracellular esterase enzymes. The of performance 2D assemblies...
Chromosomal instability resulting in copy number alterations is a hallmark of colorectal cancer (CRC). However, few studies have attempted to characterize the chromosomal changes occurring early-onset CRC order compare them with those taking place within more extensively studied late-onset subset. Our aim was genomic profiles these two groups tumors and each other. Array comparative hybridization profiling 146 (60 86 late-onset) combination an unsupervised analysis used define common...
CuFeS2 chalcopyrite nanoparticles (NPs) can generate heat under exposure to near-infrared laser irradiation. Here, we develop a protocol decorate the surface of NPs (13 nm) with thermoresponsive (TR) polymer based on poly(ethylene glycol methacrylate) combine heat-mediated drug delivery and photothermal damage. The resulting TR-CuFeS2 feature small hydrodynamic size (∼75 nm), along high colloidal stability TR transition temperature 41 °C in physiological conditions. Remarkably, NPs, when...
This work presents the first application of total-reflection X-ray fluorescence (TXRF) spectrometry, a new and powerful alternative analytical method, to evaluation bioaccumulation kinetics gold nanorods (GNRs) in various tissues upon intravenous administration mice. The parameters for developed methodology by TXRF were evaluated means parallel analysis bovine liver certified reference material samples (BCR-185R) doped with 10 μg/g gold. average values (n = 5) achieved measurements...
Considering the high incidence of Alzheimer's disease among world population over years, and costs that poses in sanitary social terms to countries, it is necessary develop non-invasive diagnostic tests allow detect early biomarkers disease. Within diagnosis methods, development contrast agents for magnetic resonance imaging becomes especially useful. Accumulating evidence suggests cholesterol may play a role pathogenesis since abnormal deposits surrounding senile plaques have been described...
Gold nanorods (GNRs) are able to efficiently convert absorbed light into localized heat within a short period of time due the surface plasmon resonance effect.This property, along with their easy bioconjugation, allows use GNRs in photothermal therapy as selective agents target cancer cells.In this study, were combined an antibody against anti-epidermal growth factor receptor (EGFR), that is frequently overexpressed brain tumors, and potential nanoconjugate (EGFR-GNRs) eliminate tumor cells...
Over the past few years, image analysis has emerged as a powerful tool for analyzing various cell biology parameters in an unprecedented and highly specific manner. The amount of data that is generated requires automated methods processing all resulting information. software available so far are suitable fluorescence phase contrast images, but often do not provide good results from transmission light microscopy due to intrinsic variation acquisition images technique itself (adjustment...
The Alternating Gradient Field Magnetometer (AGFM) is an instrument whose high sensitivity (10-8 emu) allows the detection of small amounts magnetic nanoparticles (MNPs) with accuracy. Over last few years, different techniques have been used for in vitro measurements nanostructures inside biological tissues. However, vivo studies about their distribution within body are very scarce because dispersion, after being delivered, reduces signal and hinders detection. In this paper we compare...
Lab on a Chip (LOC) farming systems have emerged as powerful tool for single cell studies combined with non‐adherent culture substrate and capture chips the study of derived tumor spheres. Cancer is characterized by its cellular heterogeneity where only small population cancer stem cells (CSCs) are responsible metastases recurrences. Thus, in vitro strategy to formation cell‐derived sphere an attractive alternative identify CSCs. In this study, we test effectiveness microdevices analysis...
Glioblastoma cancer stem-like cells seeded on substrates exhibiting surface potential differences, undergo differentiation due to the forced hyperpolarization of membrane at cell/substrate interface.
This paper presents a methodology for cell detection and counting using device that combines PDMS (polydimethylsiloxane) microfluidic multilayer channels with single solid state micropore. Optimal conditions of solid-state micropore fabrication from crystalline silicon wafers are presented. Micropores varying size can be obtained by directly etching an etchant agent concentration 50 wt% KOH, at temperatures (40, 60, 80 °C) voltages (100, 500, 1000 mV). Scanning Electron Microscopy (SEM),...
When aqueous suspensions of gold nanorods are irradiated with a pulsing laser (808 nm), pressure waves appear even at low frequencies (pulse repetition rate 25 kHz). We found that the wave amplitude depends on dynamics phenomenon. For fixed concentration and average current intensity, shows trend increasing pulse slope maximum amplitude. postulate detected ultrasonic sort shock would be generated beginning each pulse, because result positive interference all individual waves.
Introducción: Los pacientes con cáncer de cabeza y cuello (CCC) pueden presentar tumores que se originan en la mucosa y/o submucosa recubre cavidad oral, faríngea laríngea, dando lugar a síntomas impactan habitualmente deglución favorecen los problemas nutricionales estos pacientes. Hasta un 52% enfermos presentan desnutrición al diagnóstico, incrementándose hasta cerca del 90% durante terapia antineoplásica. Por lo que, son uno grupos más vulnerables para desarrollar sarcopenia asociada...
The specific goal of this research line is the development new magnetic nanoparticles-based markers for amiloide protein early detection, pre-clinic screening and disease progression assessment Alzheimer's disease. Markers have to be: highly stable in vivo; cross blood brain barrier non-destructively following intravenous injection; bind specifically plaques with high affinity produce local changes tissue contrast detectable by MRI. analysis biocompatibility nanoparticles used these studies...