A5067563039- Pluripotent Stem Cells Research
- Mesenchymal stem cell research
- Tissue Engineering and Regenerative Medicine
- CRISPR and Genetic Engineering
- RNA Interference and Gene Delivery
- MicroRNA in disease regulation
- 3D Printing in Biomedical Research
- Cancer-related gene regulation
- Extracellular vesicles in disease
- Renal and related cancers
- Cell Image Analysis Techniques
- Cancer Cells and Metastasis
- Epigenetics and DNA Methylation
- Reproductive Biology and Fertility
- Cancer Diagnosis and Treatment
- Coronary Interventions and Diagnostics
- Digital Imaging for Blood Diseases
- Innovative Microfluidic and Catalytic Techniques Innovation
- Cancer-related Molecular Pathways
- Microfluidic and Bio-sensing Technologies
- Cancer-related molecular mechanisms research
- Aortic Thrombus and Embolism
- Image Processing Techniques and Applications
- Chromosomal and Genetic Variations
- Reconstructive Surgery and Microvascular Techniques
Vitenparken
2025
Consejo Nacional de Investigaciones Científicas y Técnicas
2013-2023
Fundación para la Lucha contra las Enfermedades Neurológicas de la Infancia
2015-2023
Instituto de Neurología Cognitiva
2021-2023
University of Buenos Aires
2007-2016
Fundación Ciencias Exactas y Naturales
2007-2016
Instituto de Química y Fisicoquímica Biológicas
2015
Deep learning is a significant step forward for developing autonomous tasks. One of its branches, computer vision, allows image recognition with high accuracy thanks to the use convolutional neural networks (CNNs). Our goal was train CNN transmitted light microscopy images distinguish pluripotent stem cells from early differentiating cells. We induced differentiation mouse embryonic epiblast-like and took at several time points initial stimulus. found that can be trained recognize...
Abstract Epithelial to mesenchymal transition (EMT) is a critical cellular process that has been well characterized during embryonic development and cancer metastasis it also implicated in several physiological pathological events including stem cell differentiation. During early stages of differentiation, human cells pass through EMT where deeper morphological, molecular biochemical changes occur. Though initially considered as decision between two states, now regarded fluid exist on...
Abstract Human embryonic and induced pluripotent stem cells are self-renewing (PSC) that can differentiate into a wide range of specialized cells. Basic fibroblast growth factor is essential for PSC survival, stemness self-renewal. PI3K/AKT pathway regulates cell viability apoptosis in many types. Although it has been demonstrated activation by bFGF relevant maintenance its role on survival remains elusive. In this study we explored the molecular mechanisms involved regulation AKT. We found...
Mesenchymal stem/stromal cells (MSCs) obtained from pluripotent stem (PSCs) constitute an interesting alternative to classical MSCs in regenerative medicine. Among their many mechanisms of action, MSC extracellular vesicles (EVs) are a potential suitable substitute for future cell-free-based therapeutic approaches. Unlike cells, EVs do not elicit acute immune rejection, and they can be produced large quantities stored until ready use. Although the has already been proven, thorough...
Mesenchymal stem cells (MSCs) are a promising source of for regenerative therapies. Although they can be isolated easily from several tissues, cell expansion is limited since their properties lost with successive passages. Hence, pluripotent derived MSCs (PD-MSCs) arise as suitable alternative MSC production. Nevertheless, at present, PD-MSC derivation protocols either expensive or not clinical purposes.In this work we present therapy-grade, inexpensive and simple protocol to derive (PSCs)...
The levels of the cyclin-dependent kinase (CDK) inhibitor p21 are low in S phase and insufficient to inhibit CDKs. We show here that endogenous p21, instead being residual, it is functional necessary preserve genomic stability unstressed cells. p21depletion slows down nascent DNA elongation, triggers permanent replication defects promotes instability hard-to-replicate regions, namely common fragile sites (CFS). p21’s PCNA interacting region (PIR), not its CDK binding domain, needed prevent...
The demand for equine cloning as a tool to preserve high genetic value is growing worldwide; however, nuclear transfer efficiency still very low. To address this issue, we first evaluated the effects of time from cell fusion activation (<1h, n = 1261; 1-2h, 1773; 2-3h, 1647) on in vitro and vivo development embryos generated by cloning. Then, using different donor types two successive experiments: I) induced pluripotent stem cells (iPSCs) vs. adult fibroblasts (AF) fused ooplasts injected...
Cell plasticity is crucial in cloning to allow an efficient nuclear reprogramming and healthy offspring. Hence, cells with high plasticity, such as multipotent mesenchymal stem (MSCs), may be a promising alternative for horse cloning. In this study, we evaluated the use of bone marrow-MSCs (BM-MSCs) donors cloning, compared vitro vivo embryo development respect fibroblasts.Zona-free transfer was performed using BM-MSCs (MSC group, n=3432) or adult fibroblasts (AF n=4527). Embryos produced by...
Human foreskin fibroblasts were used to generate the iPSC line iPSC-FH2.1 using EF1a-hSTEMCCA-loxP vector expressing OCT4, SOX2, c-MYC and KLF4, in 5% O2 culture conditions. Stemness was confirmed, as pluripotency both vivo vitro, normoxia hypoxia. Embryonic Stem Cell (hESC) WA-09 reprogrammed fibroblast primary HFF-FM controls.
Abstract Background Whole blood RNA expression is modulated in response to signals from tissues, including the vessel wall. The primary objective of this study was explore ability whole transcriptomes, analysed using artificial intelligence (AI), predict coronary artery calcifications (CAC). Methods A total 196 subjects (men aged 40–70 years and women 50–70 without known cardiovascular disease (CVD)) were non-consecutively enrolled for CAC assessment via chest computed tomography. isolated...
PIWI-interacting RNAs (piRNAs) are a class of non-coding initially thought to be restricted exclusively germline cells. In recent years, accumulating evidence has demonstrated that piRNAs actually expressed in pluripotent, neural, cardiac and even cancer However, controversy remains around the existence function somatic piRNAs. Using small RNA-seq samples from H9 pluripotent cells differentiated mesoderm progenitors cardiomyocytes we identified expression 447 piRNA transcripts, which 241...
Pluripotent stem cells possess complex systems that protect them from oxidative stress and ensure genomic stability, vital for their role in development. Even though it has been reported antioxidant activity diminishes along cell differentiation, little is known about the transcriptional regulation of involved genes. The modulation some these genes led us to hypothesize could be regulated by transcription factors critical self-renewal pluripotency embryonic (ESCs) induced pluripotent...
MicroRNAs are small non-coding RNAs involved in post-transcriptional regulation of gene expression related to many cellular functions. We performed a small-RNAseq analysis cardiac differentiation from pluripotent stem cells. Our analyses identified some new aspects about microRNA this process. First, we described dynamic profile microRNAs where them clustered according their level. Second, the extensive network isomiRs and ADAR modifications. Third, families clusters establishment lineage...
Abstract The stem cell niche has a strong influence in the differentiation potential of human pluripotent cells with integrins playing major role communicating extracellular environment. However, it is not well understood how interactions between and matrix are involved cardiac differentiation. To evaluate this, we performed profile expression two stages differentiation: mesodermal progenitors cardiomyocytes. We found an active regulation different during In particular, integrin α 5 subunit...
In normal physiological conditions, restoration of a functional epidermal barrier is highly efficient; nevertheless, when it fails, one the main consequences chronic ulcerative skin defect, most frequently recognized complications diabetes. Most these venous ulcers do not heal with conventional treatment, leading to appearance infections and in patient. Treatments based on use autologous mesenchymal stem cells (MSC) have been successful; however, its implementation entails complications. The...
Cell death experiments are routinely done in many labs around the world, these backbone of assays for drug development. detection is usually performed ways, and requires time reagents. However, cell preceded by slight morphological changes shape texture. In this paper, we trained a neural network to classify cells undergoing death. We found that was able highly predict after one hour exposure camptothecin. Moreover, prediction largely outperforms human ability. Finally, provide simple python...
Embryonic stem cells (ESC) need a set of specific factors to be propagated. They can also grow in conditioned medium (CM) derived from bovine granulosa cell line BGC (BGC-CM), that not only preserves their main features but increases ESC´s proliferation rate. The mitogenic properties this were previously reported, ascribing effect an alternative spliced generated fibronectin isoform contains the extra domain A (FN EDA(+)). Here, we investigated if FN EDA(+) increased mouse and human ES...
Cell reprogramming has been well described in mouse and human cells. The expression of specific microRNAs demonstrated to be essential for pluripotent maintenance cell differentiation, but not much information is available domestic species. We aim generate horse iPSCs, characterize them evaluate the different (miR-302a,b,c,d, miR-205, miR-145, miR-9, miR-96, miR-125b miR-296). Two equine iPSC lines (L2 L3) were characterized after fibroblasts with four Yamanaka's factors...
Murine embryonic stem cells (mESCs) are pluripotent that can be propagated in an undifferentiated state continuous culture on a feeder layer or without feeders the presence of leukemia inhibitory factor (LIF). Although there has been great advance since their establishment, ESC is still complex and expensive. Therefore, finding conditions maintain self-renewal ESCs, preventing differentiation promoting proliferation, area interest. In this work, we studied effects conditioned medium from...
New therapeutic options for liver cirrhosis are needed. Mesenchymal stem cell (MSC)-derived extracellular vesicles (EVs) have emerged as a promising tool delivering factors in regenerative medicine. Our aim is to establish new that employs EVs derived from MSCs deliver fibrosis. were isolated supernatants of adipose tissue MSCs, induced-pluripotent-stem-cell-derived and umbilical cord perivascular cells (HUCPVC-EVs) by ion exchange chromatography (IEC). To produce engineered EVs, HUCPVCs...