A5050025658- Animal Genetics and Reproduction
- Advanced biosensing and bioanalysis techniques
- Reproductive Biology and Fertility
- Parkinson's Disease Mechanisms and Treatments
- Neurological disorders and treatments
- Genetic Neurodegenerative Diseases
- CRISPR and Genetic Engineering
- Mitochondrial Function and Pathology
- Biosensors and Analytical Detection
- Pluripotent Stem Cells Research
- PI3K/AKT/mTOR signaling in cancer
- Nanoparticle-Based Drug Delivery
- Renal and related cancers
- Chemical Synthesis and Analysis
- RNA Interference and Gene Delivery
- Cellular transport and secretion
- RNA Research and Splicing
- Monoclonal and Polyclonal Antibodies Research
- Neuroscience and Neuropharmacology Research
- Protein Kinase Regulation and GTPase Signaling
- ATP Synthase and ATPases Research
- Ubiquitin and proteasome pathways
- Nuclear Structure and Function
- RNA and protein synthesis mechanisms
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
Consejo Nacional de Investigaciones Científicas y Técnicas
2011-2024
National University of General San Martín
2012-2024
Centro Científico Tecnológico - San Juan
2024
Epatocentro Ticino
2014
Universidad Nacional de San Martín
2011
Administración Nacional de Laboratorios e Institutos de Salud
2001-2006
Fundación Instituto Leloir
1999-2006
University of Buenos Aires
2001
Instituto de Genética Veterinaria
1995
Fundación Ciencias Exactas y Naturales
1995
In recent years, reagentless aptamer biosensors, named aptasensors, have shown significant advancements. Particularly, electrochemical aptasensors could change the field of biosensors in this era, where digitalization seems to be a common goal many fields. Biomedical devices are integrating electronic technologies for detecting pathogens, biomolecules, small molecules, and ions, physical-chemical properties nucleic acid aptamers makes them very interesting these devices. Aptamers can easily...
The demand for equine cloning as a tool to preserve high genetic value is growing worldwide; however, nuclear transfer efficiency still very low. To address this issue, we first evaluated the effects of time from cell fusion activation (<1h, n = 1261; 1-2h, 1773; 2-3h, 1647) on in vitro and vivo development embryos generated by cloning. Then, using different donor types two successive experiments: I) induced pluripotent stem cells (iPSCs) vs. adult fibroblasts (AF) fused ooplasts injected...
Cystic fibrosis (CF), a disease caused by mutations in the cystic transmembrane regulator (CFTR) chloride channel, is associated respiratory system with accumulation of mucus and impaired lung function. The role CFTR channel regulation intracellular pathways that determine overexpression mucin genes unknown. Using differential display, we have observed expression several mRNAs may correspond to putative CFTR-dependent genes. One these was further characterized, it corresponds tyrosine kinase...
Abstract Anp32e/Cpd1, a member of the acidic nuclear phosphoprotein (Anp)32 family, is characterized by presence an amino terminal domain containing four leucine‐rich repeats and carboxyl‐terminal low‐compositional complexity region. In previous studies performed to understand biological role we showed predominant Anp32e/Cpd1 in nucleus. However, when cytoplasm, it co‐localizes spatially with protein phosphatase 2A (PP2A) near cell membranes, far from synapses. present work, show that also...
To analyze the Intracranial Hypertension (IH) development related to jugular bulb oxygen saturation (SjO2) disturbances in severe traumatic brain injury patients (sTBI).One hundred and thirty-five sTBI were reviewed. Those without IH at admission (n = 116) included. All underwent ICP SjO2 continuous monitoring. Two groups distinguished according values during first 24 hours. Group A: those with abnormal (SjO2 more than 75% or less 55%) B: normal (55-75%). Differences outcome between...
In a biological environment, nanoparticles are rapidly coated with serum proteins, which affects the NPs transport through medium, cellular uptake and response, all of can impair therapeutic efficiency. Reduction non-specific adsorption proteins is mandatory to overcome this drawback. We propose use albumin prepare multilayer coating reduce protein interactions in media. Biohybrid (bioHNPs) prepared by gold finally decorated Bombesin-related peptides (BD-bioHNPs). BioHNPs/biological media...
Abstract This study evaluates the effects of insulin-like growth factor (IGF)-1 receptor (IGF-1R) down-regulation in stimulated T lymphocytes by investigating expression early activation proteins CD69, CD25, and interleukin (IL)-2. We found that IGF-1 does not modify CD69 but increases transcription protein synthesis CD25 IL-2. The lowest level IGF-1R detected after 15 min suggested occur at initiation cell activation. was confirmed phosphorylation increased microtubule-associated kinase....
The generation of androgenetic haploid embryos enables several blastomeres to be obtained as identical copies a single spermatozoon genome. In the present study, we compared developmental ability bovine constructed by different methods, namely IVF and intracytoplasmic sperm injection (ICSI) before after oocyte enucleation. Once obtained, these were used male genome donors reconstruct biparental fusion with matured oocytes. To verify cytoplasmic contribution blastomeres, spermatozoa incubated...
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Decoration of nanoparticles with specific molecules such as antibodies, peptides, and proteins that preserve their biological properties is essential for the recognition internalization target cells. Inefficient preparation decorated leads to nonspecific interactions diverting them from desired target. We report a simple two-step procedure biohybrid containing core hydrophobic quantum dots coated multilayer human serum albumin. These were prepared by ultra-sonication, crosslinked using...
PTEN is a critical gene involved in the regulation of many cellular processes. The product this has dual phosphatase activity and able to dephosphorylate 5′ end phosphatidylinositol (3,4,5)-trisphosphate. Within nucleus, protein been associated with expression genes, although mechanism remains unclear. In paper, two specific oligonucleotide aptamers were developed selected, using SELEX procedure, according their ability detect different subcellular compartments neurons. While one aptamer was...