A5064213510- Amino Acid Enzymes and Metabolism
- Enzyme Structure and Function
- DNA Repair Mechanisms
- Bacterial Genetics and Biotechnology
- Enzyme Production and Characterization
- RNA and protein synthesis mechanisms
- Biochemical and Molecular Research
- Metabolism and Genetic Disorders
- Marine and fisheries research
- Diet and metabolism studies
- CRISPR and Genetic Engineering
- Biofuel production and bioconversion
- Marine Biology and Ecology Research
- Trace Elements in Health
- Bacteriophages and microbial interactions
- Animal Genetics and Reproduction
- Escherichia coli research studies
- Enzyme Catalysis and Immobilization
- Fire effects on ecosystems
- ATP Synthase and ATPases Research
- Bacillus and Francisella bacterial research
- Marine and coastal plant biology
- Archaeology and Natural History
- Water Quality and Resources Studies
- Endoplasmic Reticulum Stress and Disease
Liverpool John Moores University
2024-2025
University of Nottingham
2020-2023
University of Bath
2019
South Carolina Department of Natural Resources
2001
Martin Marietta Materials (United States)
1986
Abstract Recombination directionality factors (RDFs) for large serine integrases (LSIs) are cofactor proteins that control the of recombination to favour excision over insertion. Although RDFs predicted bind their cognate LSIs in similar ways, there is no overall common structural theme across LSI RDFs, leading suggestion some them may be moonlighting with other primary functions. To test this hypothesis, we searched characterized structures known RDFs. Our search shows two LSIs, TG1...
ABSTRACT Integrases from the “large serine” family are simple, highly directional site-specific DNA recombinases that have great promise as synthetic biology and genome editing tools. Integrative recombination (mimicking phage or mobile element insertion) requires only integrase two short (∼40 – 50) sites. The reverse reaction, excisive recombination, does not occur until it is triggered by presence of a second protein termed Recombination Directionality Factor (RDF), which binds...
Abstract Background In all living organisms, DNA replication is exquisitely regulated in a wide range of growth conditions to achieve timely and accurate genome duplication prior cell division. Failures this regulation cause damage with potentially disastrous consequences for viability human health, including cancer. To cope these threats, cells tightly control initiation using well-known mechanisms. They also couple synthesis nutrient richness rate through poorly understood process thought...
The glycolytic enzyme PykA has been reported to drive the metabolic control of replication through a mechanism involving moonlighting functions on essential DnaE polymerase, DnaC helicase and regulatory determinants catalytic activity in Bacillus subtilis. mutants this suffer from critical cell cycle defects, showing that plays important overall rate replication. Using biochemical approaches, we demonstrate here interacts with for modulating its when is bound primed DNA template. This...
Recombination directionality factors (RDFs) for large serine integrases (LSIs) are cofactor proteins that control the of recombination to favor excision over insertion. Although RDFs predicted bind their cognate LSIs in similar ways, there is no overall common structural theme across LSI RDFs, leading suggestion some them may be moonlighting with other primary functions. To test this hypothesis, we searched characterized structures known RDFs. Our search shows two LSIs, TG1 integrase and...
Serine integrases are phage- (or mobile element-) encoded enzymes that catalyse site-specific recombination reactions between a short DNA sequence on the phage genome (attP) and corresponding host (attB), thereby integrating into genome. Each integrase has its unique pair of attP attB sites, feature allows them to be used as orthogonal tools for modification applications. In presence second protein, Recombination Directionality Factor (RDF), catalyses reverse excisive reaction, generating...
Parageobacillus thermoglucosidasius is a thermophilic and ethanol-producing bacterium capable of utilising both hexose pentose sugars for fermentation. The organism has been proposed to be suitable the production bioethanol from lignocellulosic feedstocks. These feedstocks may difficult degrade, potential strategy optimise this process engineer strains that secrete hydrolases liberate increased amounts those However, very little known about protein transport in P. limitations process, as...
SUMMARY Cells have evolved a metabolic control of DNA replication to respond wide range nutritional conditions. Accumulating data suggest that this poorly understood depends, at least in part, on Central Carbon Metabolism (CCM). In Bacillus subtilis , the glycolytic pyruvate kinase (PykA) is intricately linked replication. This 585 amino-acid-long enzyme comprises catalytic (Cat) domain binds phosphoenolpyruvate (PEP) and ADP produce ATP, C-terminal unknown function. Interestingly, termed...
Campylobacter jejuni colonizes hosts by interacting with Blood Group Antigens (BgAgs) on the surface of gastrointestinal epithelia. Genetic variations in BgAg expression affects host susceptibility to C. . Here, we show that essential major outer membrane protein (MOMP) NCTC11168 binds Lewis b (Le ) antigen epithelia tissues and this interaction can be competitively inhibited ferric quinate (QPLEX), a chelate structurally similar bacterial siderophores. We provide evidence QPLEX inhibits...
The benthic element of the Chesapeake Bay Monitoring Program, jointly sponsored by Maryland Office Environmental Programs (OEP) and Power Plant Research Program (PPRP), is designed around concept that populations are likely to respond changes in water sediment quality resulting from specific management efforts. Both OEP PPRP monitoring programs have demonstrated organisms sensitive indicators integrators cumulative environmental effects a range anthropogenic perturbations as long major...
Cells have evolved a metabolic control of DNA replication to respond wide range nutritional conditions. Accumulating data suggest that this poorly understood depends, at least in part, on Central Carbon Metabolism (CCM). In Bacillus subtilis , the glycolytic pyruvate kinase (PykA) is intricately linked replication. This 585 amino-acid-long enzyme comprises catalytic (Cat) domain binds phosphoenolpyruvate (PEP) and ADP produce ATP, C-terminal unknown function. Interestingly, termed PEPut...