A5045436829- Legume Nitrogen Fixing Symbiosis
- Protein Kinase Regulation and GTPase Signaling
- Inflammatory mediators and NSAID effects
- Drug-Induced Hepatotoxicity and Protection
- Metal complexes synthesis and properties
- Prostate Cancer Treatment and Research
- Venomous Animal Envenomation and Studies
- Ferrocene Chemistry and Applications
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Eicosanoids and Hypertension Pharmacology
- Metalloenzymes and iron-sulfur proteins
- Pregnancy and preeclampsia studies
- Plant nutrient uptake and metabolism
- Birth, Development, and Health
- Cancer Cells and Metastasis
- Rheumatoid Arthritis Research and Therapies
- Agronomic Practices and Intercropping Systems
- Immune Response and Inflammation
- Protease and Inhibitor Mechanisms
- Skin and Cellular Biology Research
- Wastewater Treatment and Nitrogen Removal
- Cancer, Lipids, and Metabolism
- NF-κB Signaling Pathways
- Cell Adhesion Molecules Research
- Fatty Acid Research and Health
Western Sydney University
2013-2024
Ingham Institute
2013-2024
Liverpool Hospital
2014-2024
Michigan State University
2024
Camden and Campbelltown Hospitals
2013-2023
UNSW Sydney
1999-2022
St Vincent's Hospital
1993-2013
St. Vincent's Hospital
1993-2013
Saint Vincent's Catholic Medical Center
1993-2013
St. Vincent's Birmingham
2010-2013
Abstract Purpose: Cytosolic phospholipase A2-α (cPLA2-α) provides intracellular arachidonic acid to supply both cyclooxygenase and lipoxygenase pathways. We aim determine the expression activation of cPLA2-α in prostate cancer cell lines tissue effect targeting vitro vivo. Experimental Design: The was determined cells by reverse transcription-PCR, Western blot, immunocytochemistry. Growth inhibition, apoptosis, activity were after inhibition with small interfering RNA or inhibitor (Wyeth-1)....
The majority of prostate cancers are indolent, whereas a significant portion patients will require systemic treatment during the course their disease. To date, only high Gleason scores best associated with poor prognosis in cancer. No validated serum biomarker has been identified prognostic power. Previous studies showed that secretory phospholipase A2-IIa (sPLA2-IIa) is overexpressed almost all human cancer specimens and its elevated levels correlated tumor grade. Here, we found sPLA2-IIa...
Human type IIA secretory phospholipase A2 (sPLA2-IIA) is induced in association with several immune-mediated inflammatory conditions. We have evaluated the effect of sPLA2-IIA on PG production primary synovial fibroblasts from patients rheumatoid arthritis (RA). At concentrations found fluid RA patients, exogenously added dose-dependently amplified TNF-alpha-stimulated PGE2 by cultured fibroblasts. Enhancement cells was accompanied increased expression cyclooxygenase (COX)-2 and cytosolic...
Abstract Mortality from prostate cancer is associated with progression of tumors to androgen-independent growth and metastasis. Eicosanoid products both the cyclooxygenase (COX) lipoxygenase (LOX) pathways are important mediators proliferation cells in culture regulate tumor vascularization metastasis animal models. Pharmacologic agents that block either COX or LOX effectively reduce size xenografts. Phospholipase A2 (PLA2) enzymes provision arachidonic acid COX- LOX-derived eicosanoids, a...
A nodulation locus from the broad–host–range, non–legume symbiont Bradyrhizobium sp. (Parasponia) strain ANU289, has been identified by hybridisation to cloned Rhizobium trifolii ( nod ) genes. Transfer of ANU289 genes R. nodulation–deficient mutants showed that contains a functional homologue nodD gene. DNA sequence analysis revealed presence three additional nodA , nodB and nodC clustered adjacent . The four share substantial homology with those characterised narrow–host–range strains....
Tumour necrosis factor (TNF) is a pleiotropic cytokine with dual roles in cancer biology including prostate (PCa). On the one hand, there evidence that it stimulates tumour angiogenesis, involved initiation of PCa from an androgen-dependent to castrate resistant state, plays role epithelial mesenchymal plasticity, and may contribute aberrant regulation eicosanoid pathways. other TNF has also been reported inhibit neovascularisation, induce apoptosis cells, stimulate antitumour immunity. Much...
A sandwich enzyme-linked immunosorbent assay (ELISA) for human non-pancreatic phospholipase A2 (PLA2) was developed using monoclonal antibodies raised against purified recombinant PLA2 This shown to be specific showing no cross-reactivity with pancreatic or snake venom (Crotalus durissus). The immunoassay showed plasma components, and reproducible quantitative between 39 pmol 2·7 nmol PLA2/l levels of in the patients arthritis measured this immunoassay. There were significantly higher...
Biological nitrogen fixation in the Rhizobium-legume symbiosis is dependent on induction of a bacterially-encoded enzyme complex, nitrogenase. To examine organization and expression genes encoding components nitrogenase this complex system, these have been isolated from legume symbiont Rhizobium trifolii by molecular cloning. DNA sequence analysis entire nifH gene (encoding Fe-protein component nitrogenase) amino-terminal 141 codons nifD α-subunit Mo-Fe protein) indicates that are linked...
Platinum(IV) prodrugs of the [Pt(P
The oxidation of low-density lipoprotein (LDL) is thought to contribute atherogenesis. 15-Lipoxygenase (15LO) induces LDL oxidation, and phospholipase A2 enhances this process [Sparrow, C. P., Parthasarathy, S., Steinberg, D. (1988) J. Lipid Res. 29, 745−753]. As the underlying mechanism enhancing effect has not been investigated previously, we here show that in presence soybean 15LO (SLO) or human (rhLO), addition lipase, porcine pancreatic, type IIa secretory (sPLA2) greatly enhanced...