A5086644895- Cancer, Hypoxia, and Metabolism
- Epigenetics and DNA Methylation
- Autophagy in Disease and Therapy
- Cancer-related Molecular Pathways
- Histone Deacetylase Inhibitors Research
- Sirtuins and Resveratrol in Medicine
- Pluripotent Stem Cells Research
- ATP Synthase and ATPases Research
- Natural Antidiabetic Agents Studies
- Phytochemicals and Antioxidant Activities
- Glioma Diagnosis and Treatment
- Cancer Cells and Metastasis
- Nigella sativa pharmacological applications
- Cancer-related gene regulation
- Calcium signaling and nucleotide metabolism
- Cell death mechanisms and regulation
- Advances in Cucurbitaceae Research
- Tannin, Tannase and Anticancer Activities
- Genomics, phytochemicals, and oxidative stress
- Chemical Reactions and Isotopes
- Microtubule and mitosis dynamics
- RNA modifications and cancer
- Colorectal Cancer Treatments and Studies
- Gastric Cancer Management and Outcomes
- PARP inhibition in cancer therapy
University of Manitoba
2021-2025
Dalhousie University
2014-2024
CancerCare Manitoba
2023
Laboratoire de Biophotonique et Pharmacologie
2009-2018
Centre National de la Recherche Scientifique
2009-2015
Université de Strasbourg
2009-2015
University of Hong Kong
2013
Bahauddin Zakariya University
2008
University of Virginia
1997
Pluripotency is an important feature of cancer stem cells (CSCs) that contributes to self-renewal and chemoresistance. The maintenance pluripotency CSCs under various pathophysiological conditions requires a complex interaction between cellular pathways including those involved in homeostasis energy metabolism. However, the exact mechanisms maintain CSC remain poorly understood. In this report, using both human murine models CSCs, we demonstrate basal levels autophagy are required process...
Polyphenols are natural compounds widely present in fruits and vegetables, which have antimutagenic anticancer properties. The aim of the study was to determine effect a polyphenol-rich Aronia melanocarpa juice (AMJ) containing 7.15 g/L polyphenols acute lymphoblastic leukemia Jurkat cell line, and, if so, clarify underlying mechanism identify active involved. AMJ inhibited proliferation, associated with cycle arrest G2/M phase, caused induction apoptosis. These effects were an upregulation...
NAD+ is a key metabolic redox cofactor that regenerated from nicotinamide through the salvage pathway. Here, we find inhibiting pathway depletes serine biosynthesis glucose by impeding NAD+-dependent protein, 3-phosphoglycerate dehydrogenase (PHGDH). Importantly, PHGDHhigh breast cancer cell lines are exquisitely sensitive to inhibition of Further, PHGDH protein levels and those rate-limiting enzyme salvage, NAMPT, correlate in ER-negative, basal-like cancers. Although inhibitors actively...
Abstract Despite tremendous research efforts, successful targeting of aberrant tumor metabolism in clinical practice has remained elusive. Tumor heterogeneity and plasticity may play a role the failure metabolism-targeting interventions for treating cancer patients. Moreover, compensatory growth-related processes adaptive responses exhibited by heterogeneous subpopulations to metabolic inhibitors are poorly understood. Here, using clinically-relevant patient-derived glioblastoma (GBM) cell...
Abstract Background The mitochondrial pyruvate carrier (MPC), a central metabolic conduit linking glycolysis and metabolism, is instrumental in energy production. However, the role of MPC cancer controversial. In particular, importance glioblastoma (GBM) disease progression following standard temozolomide (TMZ) radiation therapy (RT) remains unexplored. Methods Leveraging vitro vivo patient-derived models TMZ-RT treatment GBM, we characterize temporal dynamics abundance downstream...
Class I major histocompatibility complex (MHC-I)-bound peptide ligands dictate the activation and specificity of CD8+ T cells thus are important for devising T-cell immunotherapies. In recent times, advances in mass spectrometry (MS) have enabled precise identification these MHC-I peptides, wherein MS spectra compared against a reference proteome. Unfortunately, matching to proteome databases is hindered by inflated search spaces attributed lack enzyme restriction searches, limiting...
Cancer stem-like cells (CSCs), a small population of pluripotent residing within heterogeneous tumor mass, remain highly resistant to various chemotherapies as compared the differentiated cancer cells. It is being postulated that CSCs possess unique molecular mechanisms, such autophagic homeostasis, allow withstand therapeutic assaults. Here we demonstrate HDAC6 inhibition differentially modulates macroautophagy/autophagy in Using human and murine CSC models cells, show or knockdown (KD)...
Tumor-associated immunosuppression aids cancer cells to escape immune-mediated attack and subsequent elimination. Recently, however, many oncolytic viruses, including reovirus, have been reported overturn such promote the development of a clinically desired antitumor immunity, which is known favorable patient outcomes. Contrary this existing paradigm, in article we demonstrate that reovirus augments tumor-associated immediately following its therapeutic administration. Our data show induces...
NAD+ metabolism plays key roles not only in energy production but also diverse cellular physiology. Aberrant is considered a hallmark of cancer. Recently, the tumor suppressor p53, major player cancer signaling pathways, has been implicated as an important regulator metabolism. This notion led us to examine whether p53 can regulate biosynthesis cell. Our search resulted identification nicotinamide mononucleotide adenylyltransferase 2 (NMNAT-2), synthetase, novel downstream target gene p53....
Stem-like cancer cells, with characteristic self-renewal abilities, remain highly refractory to various clinical interventions. As such, stemness-inhibiting entities, such as tumor suppressor p53, are therapeutically pursued for their anticancer activities. Interestingly, similar implications TAp73 in regulating stemness features within stem-like cells unknown.Experimental Design: This study utilizes vitro molecular biology techniques, including immunoblotting, qRT-PCR, and mass...
Abstract Group 3 medulloblastoma (G3 MB) carries the worst prognosis of all MB subgroups. MYC oncoprotein is elevated in G3 tumors; however, mechanisms that support abundance remain unclear. Using metabolic and mechanistic profiling, we pinpoint a role for mitochondrial metabolism regulating MYC. Complex-I inhibition decreases MB, attenuates expression MYC-downstream targets, induces differentiation, prolongs male animal survival. Mechanistically, complex-I increases inactivating acetylation...
Abstract Cancer cells with a less differentiated stem-like phenotype are more resistant to therapeutic manipulations than their counterparts, and considered as one of the main causes cancer persistence relapse. As such, induction differentiation in (CSLCs) has emerged an alternative strategy enhance efficacy anticancer therapies. CSLCs metabolically distinct from cells, any aberration intrinsic metabolic state can induce CSLCs. Therefore, metabolism-related molecular targets, capacity...