A5014177719- Fungal Infections and Studies
- Antifungal resistance and susceptibility
- Respiratory viral infections research
- Nail Diseases and Treatments
- Viral gastroenteritis research and epidemiology
- Wound Healing and Treatments
- SARS-CoV-2 and COVID-19 Research
- Virology and Viral Diseases
- Toxin Mechanisms and Immunotoxins
- Polysaccharides and Plant Cell Walls
- Influenza Virus Research Studies
- SARS-CoV-2 detection and testing
- Animal Virus Infections Studies
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Surgical site infection prevention
- Biosensors and Analytical Detection
- Immunodeficiency and Autoimmune Disorders
- RNA Research and Splicing
- Mosquito-borne diseases and control
- Thermal Regulation in Medicine
- Barrier Structure and Function Studies
- COVID-19 Clinical Research Studies
- Metal Forming Simulation Techniques
- Optical measurement and interference techniques
- Pediatric health and respiratory diseases
The University of Tokyo
2024-2025
Tohoku University
2018-2024
Sendai Medical Center
2022-2024
National Hospital Organization
2024
Ritsumeikan University
2017
Interferon (IFN)-γ is mainly secreted by CD4+ T helper 1 (Th1), natural killer (NK) and NKT cells after skin injury. Although IFN-γ well known regarding its inhibitory effects on collagen synthesis fibroblasts in vitro, information limited role wound healing vivo. In the present study, we analyzed how defect of affects healing. Full-thickness wounds were created backs wild type (WT) C57BL/6 IFN-γ-deficient (KO) mice. We percent closure, breaking strength, accumulation leukocytes, expression...
Morbilliviruses, including measles virus (MV), canine distemper (CDV), and cetacean morbillivirus, pose a significant threat to humans animals. While the host range of morbilliviruses is generally well-defined, severe cross-species transmission events also have been reported.& Their entry into immune cells, primary targets morbilliviruses, relies on signaling lymphocytic activation molecule (SLAM), receptor whose species-specific variations influence viral range. However, extent which...
Respiratory syncytial virus (RSV) is a seasonal respiratory pathogen that primarily affects young children, potentially causing severe lower tract disease. Despite the high disease burden, understanding of RSV pathophysiology remains limited. To address this, advanced infection models are needed. Whereas HEp-2 cells widely used because their susceptibility to RSV, they do not accurately reflect host response human tract. In this study, we evaluated human-induced pluripotent stem cell-derived...
The cell walls and capsules of Cryptococcus neoformans , a yeast-type fungal pathogen, are rich in polysaccharides. Dectin-2 is C-type lectin receptor (CLR) that recognizes high-mannose
IL-17A is a proinflammatory cytokine produced by many types of innate immune cells and Th17 involved in the elimination extracellularly growing microorganisms, yet role this host defense against intracellularly microorganisms not well known. Cryptococcus deneoformans an opportunistic intracellular growth fungal pathogen that frequently causes fatal meningoencephalitis patients with impaired responses. In current study, we analyzed C. infection. was quickly γδT at phase infected lungs....
Cryptococcus neoformans is rich in polysaccharides of the cell wall and capsule. Dectin-2 recognizes high-mannose plays a central role immune response to fungal pathogens. Previously, we demonstrated was involved activation dendritic cells upon stimulation with C. neoformans, suggesting existence ligand recognized by Dectin-2. In present study, examined structures contributing Dectin-2-mediated cells. NFAT-GFP reporter assay reported expressing Dectin-2, lysates, but not whole yeast cells,...
Cryptococcus deneoformans is an opportunistic fungal pathogen that frequently causes fatal meningoencephalitis in patients with impaired cell-mediated immune responses such as AIDS. Caspase-associated recruitment domain 9 (CARD9) plays a critical role the host defense against cryptococcal infection, suggesting involvement of one or more C-type lectin receptors (CLRs). In present study, we analyzed macrophage-inducible (Mincle), CLRs, C. infection. Mincle expression lungs wild-type (WT) mice...
Abstract Human parainfluenza viruses (hPIVs) are causative agents of upper and lower respiratory tract infections they have four serotypes. The virion surface displays hemagglutinin‐neuraminidase (HN), having hemagglutinating (HA) neuraminidase (NA) activities in a single molecule. HA activity binds the to sialic acid on viral receptor host cells NA releases progeny from cell surface. There several methods for assaying activity, such as thiobarbituric assay, 4‐methylumbelliferyl‐ N...
Viral RNA synthesis of mononegaviruses occurs in cytoplasmic membraneless organelles called inclusion bodies (IBs). Here, we report that IBs mumps virus (MuV), which is the causative agent and belongs to family Paramyxoviridae , displayed liquid organelle properties formed by liquid-liquid phase separation. Super-resolution microscopic analysis MuV demonstrated nucleocapsid phospho (P)-proteins a cage-like structure viral polymerase adopted reticular pattern colocalized with RNAs. In...
Virus isolates are not only useful for diagnosing infections, e.g., respiratory syncytial virus (RSV), but can also facilitate many aspects of practical viral studies such as analyses antigenicity and the action mechanisms antivirals, among others. We have been isolating RSV from clinical specimens patients with symptoms every year since our first isolation in 1964, rates varied considerably over years. As collected conventionally stored a refrigerator collection to inoculation into cells,...
Abstract Our previous findings indicated that many respiratory syncytial virus (RSV) isolates are unstable at 4 °C compared to 20 °C. Some of the strains completely lose infectivity after 24 h This study analyzed inactivation process using a representative strain, RSV/Sendai/851/13. After storage °C, was inactivated but retained its ability attach and be taken into host cells. It suggested reduced fusion between viral cellular membranes. During RSV (F) protein underwent conformational change...
Cryptococcus deneoformans is an opportunistic fungal pathogen that infects the lungs via airborne transmission and frequently causes fatal meningoencephalitis. Claudins (Cldns), a family of proteins with 27 members found in mammals, form tight junctions within epithelial cell sheets. Cldn-4 18 are highly expressed airway tissues, yet roles these claudins respiratory infections have not been clarified. In present study, we analyzed lung-specific Cldn-18 (luCldn-18) host defense against C....
A critical aspect of the mechanism severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection is protease-mediated activation viral spike (S) protein. The type II transmembrane serine protease TMPRSS2 crucial for SARS-CoV-2 in lung epithelial Calu-3 cells and murine airways. However, importance needs to be re-examined because ability utilize significantly reduced Omicron variants that spread globally. For this purpose, replication profiles were analyzed human organoids. All...
Immunofluorescence methods to detect seasonal human coronavirus (HCoV)-OC43 in nasopharyngeal swab specimens using cell lines have not yet been established. A rectal adenocarcinoma line (HRT-18) was exposed the obtained from patients with upper respiratory tract infections. staining conducted combination of serum containing HCoV-OC43 anti-spike protein antibody and a fluorescence-labeled anti-human antibody. Positive HRT-18 cells detected after exposure nine eleven which RNA FilmArray...
is a yeast-type fungus that causes fatal meningoencephalitis in immunocompromised patients and evades phagocytic cell elimination through an escape mechanism. Memory T (Tm) cells play central role preventing the reactivation of this fungal pathogen. Among these cells, tissue-resident memory (T
周囲への感染リスクを有するCOVID-19感染者を検出するために迅速抗原検査を用いたスクリーニングを行うことの妥当性の有無を知る目的で,COVID-19患者/疑い患者ならびに無症状の患者濃厚接触者から採取した鼻咽頭拭い検体の中で定量的リアルタイムRT-PCR検査でSARS-CoV-2遺伝子が検出された162検体について,ウイルス分離を試み分離陽性検体を101検体得た.また,これらの162検体について本邦で市販されていた5社5種類の簡易迅速抗原検出キット製品を用いた抗原検査を試みた.PCRで得られた検体中のウイルス遺伝子コピー数のデータとウイルス分離成績の結果をあわせ,用いた製品ごとに解析した.その結果,被験者の症状の有無にかかわらず,すべてが感染性ウイルスが分離された検体の95%以上に陽性を示し,このような検査対象集団においては迅速抗原検査に感染伝播リスクの見逃しが少ないことがわかった.