A5059125131- Enzyme Structure and Function
- DNA and Nucleic Acid Chemistry
- Molecular Biology Techniques and Applications
- Legionella and Acanthamoeba research
- DNA Repair Mechanisms
- Retinal Development and Disorders
- Advanced Chemical Sensor Technologies
- Cellular transport and secretion
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Radioactive Decay and Measurement Techniques
- Peptidase Inhibition and Analysis
- Microtubule and mitosis dynamics
- Metalloenzymes and iron-sulfur proteins
- Adenosine and Purinergic Signaling
- Meat and Animal Product Quality
- Metabolomics and Mass Spectrometry Studies
- Heme Oxygenase-1 and Carbon Monoxide
- Studies on Chitinases and Chitosanases
- Spectroscopy and Chemometric Analyses
CIC energiGUNE
2024
CIC bioGUNE
2017-2024
GAIKER Technology Centre
2024
Microbial pathogens employ sophisticated virulence strategies to cause infections in humans. The intracellular pathogen Legionella pneumophila encodes RidL hijack the host scaffold protein VPS29, a component of retromer and retriever complexes critical for endosomal cargo recycling. Here, we determined crystal structure L. complex with human VPS29-VPS35 subcomplex. A hairpin loop protruding from inserts into conserved pocket on VPS29 that is also used by cellular ligands, such as...
Abstract Endosomal trafficking ensures the proper distribution of lipids and proteins to various cellular compartments, facilitating intracellular communication, nutrient transport, waste disposal, maintenance cell structure. Retromer, a peripheral membrane protein complex, plays an important role in this process by recruiting associated actin‐polymerizing WASH complex establish distinct sorting domains. The is recruited through interaction VPS35 subunit retromer with FAM21. Here, we report...
p15PAF is an oncogenic intrinsically disordered protein that regulates DNA replication and lesion bypass by interacting with the human sliding clamp PCNA. In absence of DNA, traverses PCNA ring via extended PIP-box contacts surface. Here, we probed atomic-scale structure p15PAF-PCNA-DNA ternary complexes. Crystallography MD simulations show that, when occupies two subunits homotrimer, within channel binds unoccupied subunit. The PCNA-bound in presence invariant, solution NMR confirms does...
Human DNA polymerase δ is essential for replication and acts in conjunction with the processivity factor proliferating cell nuclear antigen (PCNA). In addition to its catalytic subunit (p125), pol comprises three regulatory subunits (p50, p68, p12). PCNA interacts all of these subunits, but only interaction p68 has been structurally characterized. Here, we report solution NMR–, isothermal calorimetry–, X-ray crystallography–based analyses p12–PCNA interaction, which takes part modulation...
Nowadays, the meat industry requires non-destructive, sustainable, and rapid methods that can provide objective accurate quality assessment with little human intervention. Therefore, present research aimed to create a model classify beef samples from longissimus thoracis muscle according their tenderness degree based on hyperspectral imaging (HSI). In order obtain different textures, two main strategies were used: (a) aging type (wet dry or without starters) (b) times (0, 7, 13, 21, 27...
Abstract Endosomal trafficking ensures the proper distribution of lipids and proteins to various cellular compartments, facilitating intracellular communication, nutrient transport, waste disposal, maintenance cell structure. Retromer, a peripheral membrane protein complex, plays an important role in this process by recruiting associated actin- polymerizing WASH complex establish distinct sorting domains. The is recruited through interaction VPS35 subunit retromer with FAM21. Here, we report...