This study developed and validated an analytical method for simultaneous identification quantification of 41 veterinary drugs in honey using liquid extraction (LLE) chromatography coupled to quadrupole-Orbitrap high-resolution mass spectrometry (LC-Q-Orbitrap HRMS). The was accordance with Commission Implementing Regulation (CIR) EU 2021/808 at five different concentrations ranging from 0.075 50 μg/kg. mean recoveries ranged 70 105, while repeatability values were all below 17%. linearity,...

Acetamiprid (neonicotinoid insecticide) and azoxystrobin (fungicide) are widespread pesticides used for pest management, but they have the potential toxicity to mammals. The goal of this study was look oxidative stress, metabolic alterations, reproductive problems in male rats' serum after 2 months exposure sub-lethal dosages acetamiprid azoxystrobin. Seven classes rats were formed: control, 3 groups (1/10, 1/20, 1/40 LD50), LD50) orally daily treated (n = 8/group). Our findings revealed...

Validation of a High-Resolution Orbitrap (HR-Orbitrap) method for the simultaneous identification, confirmation, and quantification seven 5-Nitroimidazoles (5-NDZs) in meat casing first time to our knowledge. The was validated under Commission Decision (CD) 2002/657/EC satisfied its requirements Minimum Method Performance Requirements (MMPRs) 1 µg/kg these Compounds casing. samples were extracted using modified QuEChERS method. analyte recovery ranging 75.4–104.8% 72.44–103.53% casing, with...

This study focused on evaluating and optimising the liquid-liquid extraction (LLE) method for multi-residue analysis of seven AGs in bovine liver, meat, milk, kidney conjunction with liquid chromatography coupled to a quadrupole-Orbitrap high-resolution mass spectrometry (LC-Q-Orbitrap HRMS) quantification. Of all hydrophilic interaction (HILIC) stationary phases tested, ZIC-HILIC column yielded best performance. The was validated according Commission Implementing Regulation (CIR) EU...

Nitroimidazoles are well-known antibacterial and antiprotozoal agents, effective against various infections. However, they may also exhibit genotoxic, carcinogenic mutagenic effects. This study aimed to develop an analytical method quantify nitroimidazole residues their metabolites in honey using Ultra Performance Liquid Chromatography coupled with Orbitrap High-Resolution Mass Spectrometry (UPLC-Orbitrap-HRMS) validate it according Commission Implementing Regulation (EU) 2021/808. The...

An analytical method was developed for identification and quantification of zilpaterol in bovine liver, meat, heart, kidney, using liquid chromatography coupled to quadrupole-Orbitrap high-resolution mass spectrometry (LC-Q-Orbitrap HRMS). It validated accordance with Commission Implementing Regulation (CIR) EU 2021/808 at six different concentrations, ranging from 0.1 5 μg/kg. The mean recoveries ranged 71% 99%, while the decision limit (CCα) detection capability (CCβ) ranges were 0.11-0.12...

This study was carried out to develop an accurate analytical method for the determination of 17 Sulfonamides (SAs), 4 Tetracyclines (TCs) and Chloramphenicol (CAP) residues be used in a monitoring program analysis studied veterinary drugs chicken samples from different poultry farms Egypt. Instrument linearity established using multi-level calibration curve (1 100) μg/L (0.1 20) Chloramphenicol; correlation coefficient ≥ 0.995 all compounds. Methods has been concentration levels which lie...

This paper reports a practical approach for the stability testing of 37 veterinary drugs in stock standard solutions stored at -20°C 1-3 years and study expiry date extension 7 expired reference materials 4°C. Stored were compared versus freshly prepared concentrations determined using LC-MS/MS. The validity was tested by new valid materials. LC-MS/MS method parameters optimised to get maximum signal analytes. Statistical analysis developed performed evaluate results according acceptability...

Endocrine-disrupting compounds as pesticides affect the hormonal balance, and this can result in several diseases. Therefore, analysis of representative hormones with acetamiprid (AC) azoxystrobin (AZ) was a good strategy for investigation endocrine-disrupting activity pesticides. Hence, sensitive rapid analytical method using liquid chromatography-tandem mass spectrometry (LC-MS/MS) developed. The validated AC, AZ, estriol, estrone, progesterone, testosterone serum, testis, liver rats....

In this study, a detection of antibiotic and pesticide residues in honey bees (Apis mellifera lamarckii) samples collected from the Egyptian local market followed by evaluation human health risk assessment for both adults children associated with consumption as result contamination calculation parameters such HQ HI.Modified method was used antibiotics analysis shows acceptable recovery 97% tested between 70 115% while only 3% recovered 60-69% RSD 21% accordance EU guidelines 2002/657/EC.The...

An analytical method for determination of inorganic bromide in cereals, herbs, fruits and vegetables had been validated. The comminuted samples are suspended an acidified aqueous solution propylene oxide, with being simultaneously extracted. Organic is converted to mixture 1-bromo-2-propanol 2-bromo-1-propanol partitioned by ethyl acetate then determined GC-ECD without further cleanup, the parameters such as limit quantification linearity have investigated. found be 2 mg/kg dry 0.2mg/kg...

A sensitive method for the determination, confirmation and validation of sum malachite green (MG), leucomalachite (LMG) crystal violet (CV) leucocrystal in fish muscle has been developed.It is based on liquid-liquid extraction by acetonitrile followed liquid chromatography-tandem mass spectrometry (LC-MSMS) with electrospray ion source (ESI) positive mode determination (malachite green, leuco violet, violet).The simple, fast inexpensive simultaneous analysis selected compounds.Fish samples...