Berberine (BBR) is widely used to treat gastrointestinal diseases. However, the pharmacological mechanism of action BBR in anti-chronic atrophic gastritis (CAG) remains unclear. This study aimed investigate CAG by integration molecular biology and multi-omics studies strategy. The model was established alternating drinking water 0.1% ammonia 20 mmol/L sodium deoxycholate, accompanied an irregular diet. Serum biochemical indices including PGI, PGII, GAS-17, IL-6, IL-1β, TNF-α were analyzed....

Background: Gastric ulcers (GUs) are prevalent digestive disorders worldwide. Wuzhuyu Decoction (WZYT) is a traditional Chinese medicine that has been employed for centuries to alleviate ailments like indigestion and vomiting. This study aims explore the potential effects underlying mechanisms of WZYT on alcohol induced gastric ulcer treatment. Methods: We macroscopic assessment evaluate index (UI), while enzyme-linked immunosorbent assay (ELISA) was utilized detecting biochemical...

Background: The Zuojin Pill (ZJP) is widely used for treating chronic atrophic gastritis (CAG) in clinical practice, effectively ameliorating symptoms such as vomiting, pain, and abdominal distension patients. However, the underlying mechanisms of ZJP CAG has not been fully elucidated. Purpose: This study aimed to clarify characteristic function treatment its potential mechanism. Methods: model was established by alternant administrations ammonia solution sodium deoxycholate, well an...

Identifying predictors of mortality from diabetes mellitus (DM) and heart disease can help shape treatment strategies. Presence androgenetic alopecia (AGA) might be such a predictor.To determine whether the presence AGA is associated with an elevated rate DM in both sexes after adjustment for potential confounders.A population-based prospective cohort study.Community-based integrated screening Taiwan.A total 7252 subjects aged 30 to 95 years participated baseline survey using Norwood Ludwig...

Aim. The aim of this study was to explore the antipyretic effect and potential mechanism Huanglian Jiedu Decoction (HLJDD) on LPS-induced fever in rats. Materials Methods. rat model established by LPS. Anal temperature rats measured every 1 hour after modeling. TNF-α, IL-6, PGE2, cAMP serum or hypothalamus tissue were detected ELISA kit. In order active ingredients HLJDD, we predicted underlying using network pharmacology then verified its Western Blotting. Results. results showed that HLJDD...

Objective: The objective of the study was to explore mechanism action Wuzhuyu decoction (WZYD) in treating chronic atrophic gastritis (CAG). Materials and Methods: Intersectional target genes WZYD, CAG, their differential metabolites were obtained. Search Tool for Retrieval Interacting Genes/Proteins (STRING) used construct a protein–protein interaction network targets, critical WZYD treatment CAG screened. Functional pathway enrichment analyses performed complete network. Pathological tests...

Coptisine (COP), a naturally occurring alkaloid, is recognized for its varied pharmacological impacts and supportive function in intestinal well-being. However, the role of COP to protect colonic epithelium colitis has not been extensively investigated. The objective this study was assess efficacy ameliorating by investigating histopathology, mucosal barrier function, transient receptor potential (TRP) signaling pathways mice with colon disease compared control group, thereby elucidating...

Purpose: The purpose of this analysis was to assess the utility Pitx2+/− mice as a model for ocular features Axenfeld-Rieger Syndrome and congenital glaucoma. Methods: Eyes wild-type littermates were examined clinically using optical coherence tomography (OCT) fundus photography. Intraocular pressures measured TonoLab rebound tonometer. histologically PITX2 expression, structural integrity, optic nerve ganglion cell content. Results: is present postnatally in corneal endothelium stroma, iris...

Background: Paeoniflorin (PF), the major active compound isolated from roots of Paeonia lactiflora Pall., has been used in treatment severe hepatic diseases for several decades and displays bright prospects liver protective effect. However, its biological mechanism that regulates bile acid metabolism cholestatic injury not fully elucidated. Our study aims to investigate by which PF using a comprehensive approach combining metabolomics network pharmacological analysis. Methods: The...

Background: Farnesoid X receptor (FXR) is a key metabolic target of bile acids (BAs) and also for drugs against several liver diseases. However, the contribution FXR in pathogenesis cholestasis still not fully understood. The purpose this study to provide comprehensive insight into properties FXR-involved mice. Materials methods: In study, an alpha-naphthylisothiocyanate (ANIT)-induced mouse model FXR-/- mice were established investigate effect on cholestasis. ileal pathology was evaluated....

To investigate the role of disulfide bonds in capsid structure, a recombinant JC virus‐like particle (VLP) was used. The major protein, VP1, virus expressed yeast cells. yeast‐expressed VP1 self‐assembled into VLP. Disulfide were found VLP which caused dimeric and trimeric linkages as demonstrated by non‐reducing SDS–PAGE. remained intact when reduced dithiothreitol. without could be disassembled capsomeres EGTA alone, but those with not EGTA. Capsomeres reassembled VLPs presence calcium...

The aim of the present study was to identify potential prognostic microRNA (miRNA) biomarkers for colon adenocarcinoma (COAD) prediction using dataset Cancer Genome Atlas (TCGA). genome‑wide miRNA sequencing and corresponding COAD clinical information were downloaded from TCGA. Prognosis‑related screening performed by multivariable Cox regression analysis used signature construction. Ten miRNAs (hsa‑mir‑891a, hsa‑mir‑6854, hsa‑mir‑216a, hsa‑mir‑378d‑1, hsa‑mir‑92a‑1, hsa‑mir‑4709,...

Paeoniflorin (PF), a water-soluble monoterpene glycoside, is initially isolated from the dried roots of Paeonia lactiflora Pall., which has effects on ameliorating cholestasis in our previous study. However, comprehensive approaches for understanding protective and mechanisms underlying cholestatic liver injury regulating bile acid metabolism have not been sufficiently elucidated. This study was aimed to explore effectiveness as well potential mechanism PF alpha-naphthylisothiocyanate...

Abstract Background and Aim Small heterodimer partner (SHP, encoded by NR0B2) plays an important role in maintaining bile acid homeostasis. The loss of the hepatic farnesoid X receptor (FXR)/SHP signal can cause severe cholestatic liver injury (CLI). FXR SHP have overlapping nonoverlapping functions However, key played CLI is unclear. Methods In this study, alpha‐naphthylisothiocyanate (ANIT)‐induced cholestasis mouse model was established. effect knockout (SHP‐KO) on ileal pathology...

ABSTRACT Coptisine (COP), a naturally occurring alkaloid, is known for its diverse pharmacological effects and supportive role in intestinal health. Despite this, the detailed mechanisms behind therapeutic benefits are not yet fully understood. The objective of this study to investigate potential COP treatment Ulcerative Colitis (UC) delineate critical pathways by which it exerts effects. To assess COP's effectiveness, mice were administered monitored clinical symptoms, activity, disease...

Small hepatocellular carcinoma (HCC) affects millions of individuals worldwide. Surveillance high-risk patients increases the early detection small HCC.To identify prognostic factors affecting overall survival (OS) and recurrence-free (RFS) with HCC.The present prospective study enrolled 140 Taiwanese stage I or II HCC. Clinical parameters interest included operation type, tumour size, histology, Child- Pugh class, presence hepatitis B surface antigen liver cirrhosis, C status,...