A5053089414- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- Cancer-related molecular mechanisms research
- Cancer Genomics and Diagnostics
- RNA Research and Splicing
National University of Singapore
2022-2025
National University Cancer Institute, Singapore
2022-2024
Most mammalian genes generate messenger RNAs with variable untranslated regions (UTRs) that are important post-transcriptional regulators. In cancer, shortening at 3' UTR ends via alternative polyadenylation can activate oncogenes. However, internal splicing remains poorly understood as studies have traditionally focused on protein-coding alterations. Here we systematically map the pan-cancer landscape of and present this in SpUR ( http://www.cbrc.kaust.edu.sa/spur/home/ ). is widespread,...
Colorectal cancer (CRC) ranks as the second leading cause of deaths globally. In recent years, short-read single-cell RNA sequencing (scRNA-seq) has been instrumental in deciphering tumor heterogeneities. However, these studies only enable gene-level quantification but neglect alterations transcript structures arising from alternative end processing or splicing. this study, we integrated short- and long-read scRNA-seq CRC samples to build an isoform-resolution transcriptomic atlas. We...
Abstract Background: Gastric cancer (GC) with peritoneal metastases (PM) carries a grim prognosis. Despite progress in elucidating transcriptomic mechanisms of metastasis, epigenetic factors and vulnerabilities are still not fully understood. Methods: Primary tumor (PT, n=157), primary normal (PN, n=33) (PM, n=97) from patients GC were retrieved sequenced whole transcriptome sequencing. PSI-Sigma was conducted to retrieve alternative pre-mRNA splicing (AS) changes. Differential events (DSE)...
Abstract Purpose: Peritoneal metastases (PM) in colorectal cancer (CRC) portend a poor prognosis. We sought to elucidate molecular features differentiating primary tumors (PTs) from PMs and actionable targets facilitating transcoelomic dissemination progression. Experimental Design: performed multi-omic profiling of 227 samples 136 patients, including 56 tumor (PT) 120 synchronous comprising 34 matched PT-PM pairs. Whole exome, bulk RNA-seq analysis was conducted identify underlying genomic...
Abstract Colorectal cancer (CRC) is the second leading cause of death worldwide. In recent years, short-read single-cell RNA sequencing (scRNA-seq) has been instrumental in deciphering tumor cell heterogeneities. However, these studies only enable gene-level expression quantification but neglect alterations transcript structures, which arise from alternative end processing or splicing, and are frequently observed cancer. this study, we integrated short- long-read scRNA-seq CRC patient...
Abstract Colorectal cancer (CRC), a complex and molecularly heterogenous disease that lacks robust predictive markers targeted therapies, is the second leading cause of death worldwide. In recent years, technological advances in short-read single-cell RNA sequencing (scRNA-seq) have been instrumental deciphering tumor cell heterogeneities. However, most studies focused on gene-level expression quantification without considering alterations transcript structures arising from widespread...