Abstract Background In our previous investigation, we revealed a significant increase in the expression of microRNA-6881-3p (miR-6881-3p) follicular fluid granulosa cells (GCs) from women with diminished ovarian reserve (DOR) compared to those normal (NOR). However, role miR-6881-3p development DOR remains poorly understood. Objective This study aimed elucidate involvement regulation function and pathogenesis DOR. Materials methods Initially, assessed levels GCs obtained human both NOR cases...

Abstract Introduction Laser-assisted hatching (LAH) is a commonly used adjunct technique; however, its effectiveness has not been fully established. Objective We evaluated the effects of LAH on pregnancy outcomes in frozen-thawed embryo transfer (FET) cycles cleavage-stage embryos. Materials and methods This retrospective study involved 5779 FET performed at Reproductive Genetic Center Affiliated Hospital Shandong University Traditional Chinese Medicine between January 2016 December 2020....

To investigate BMAL1 and BMP6 expressive differences in ovarian granulosa cells (OGCs) of patients with polycystic ovary syndrome (PCOS), explore regulatory relationship, assess their impacts on OGC proliferation apoptosis, analyze correlations ART outcomes patients. A clinical study selected 40 PCOS who underwent IVF/ICSI our hospital from January to October 2022 39 controls male or tubal factor infertility. RT-qPCR Western blot assessed mRNA/protein levels. The number oocytes retrieved,...

Objective To investigate the efficacy of Er Zhi Tian Gui Formula (EZTGF) in diminished ovarian reserve (DOR) patients undergoing vitro fertilization-embryo transfer (IVF-ET); to examine pharmacological mechanism EZTGF inhibiting granulosa cell (OGC) apoptosis. Methods A total 120 DOR IVF-ET Affiliated Hospital Shandong University TCM from March December 2021 were randomly assigned (n = 60) or Placebo groups. All participants followed a gonadotropin-releasing hormone antagonist protocol for...

Infertility is a global reproductive-health problem, and diminished ovarian reserve (DOR) one of the common causes female infertility. Long noncoding RNAs (lncRNAs) are crucial regulators numerous physiological pathological processes in humans. However, whether lncRNAs involved development DOR remains to be elucidated.Ovarian granulosa cells (OGCs) extracted from infertile women with normal (NOR) were subjected high-throughput sequencing. Comprehensive bioinformatics analysis was conducted...

Despite a large number of studies on the selection trigger drugs, it remains unclear whether dual with human chorionic gonadotropin (hCG) and gonadotropin-releasing hormone (GnRH) agonist, compared to hCG alone, can improve reproductive outcome patients undergoing assisted technology. Therefore, this study aimed compare laboratory clinical outcomes versus single trigger.In retrospective cohort study, we evaluated 520 in vitro fertilization/intracytoplasmic sperm injection (IVF/ICSI) cycles...

This retrospective study aimed to explore whether puncturing and aspirating asynchronized large follicles during long GnRH-a protocol COH impacted IVF-ET outcomes.A total of 180 patients with were retrospectively analyzed. They divided into a puncture group, Group 1 (n = 81), non-puncture 2 99), according aspiration performed on the prematurely developing follicles. The data selected statistically analyzed assess effect follicle ovulation induction final pregnancy results. In addition, we...

Abstract Background/Aim Cangfu Daotan Wan (CFDTW) has been widely used for polycystic ovary syndrome (PCOS) patients in the type of stagnation phlegm and dampness. In this study, we aimed to evaluate mechanism underlying therapeutic effect CFDTW on PCOS with phlegm-dampness (PDS). Methods silico analysis was adopted identify potential targets downstream pathways treatment PCOS. Expression PKP3 examined ovarian granulosa cells from PDS rat models induced by dehydroepiandrosterone (DHEA)....

Abstract Purpose To evaluate whether PTX3 is differentially expressed in the granulosa lutein cells derived from women with PCOS and BMP6 can regulate expression of hGL cells. Methods The levels were evaluated by RT-qPCR. correlation between /PTX3 oocyte quality indexes analyzed using clinical samples. incubated at different concentrations times to check KGN TGF-β type I inhibitors small interfering RNA targeting ALK2/3/6,SMAD1/5/8 SMAD4 used study involvement SMAD dependent pathways Results...

In contemporary times, the employment of vitrification freezing technology has led to widespread adoption frozen-thawed embryo transfer (FET) worldwide. Meanwhile, hormone replacement therapy (HRT) is a crucial protocol for priming endometrium during FET cycles. Estrogen required in HRT cycles induction progesterone receptors and promote endometrial thickness. However, there no universal consensus on treatment duration, dosage regimen, administration route, target serum estrogen levels....

Abstract Background Previous studies have shown that frozen embryo transfer (FET) resulted in increased live birth rates (LBR) and reduced the risk of ovarian hyperstimulation syndrome (OHSS) than did fresh women with polycystic ovary (PCOS). In addition, overweight/obese PCOS are at subfertility complications pregnancy, compared normal-weight women. The stimulation artificial hormone regimes two more commonly used endometrial preparation protocols patients.This retrospective study aims to...

Objective . This was a population‐based cohort study, to compare the type and structure of intestinal flora in patients with polycystic ovary syndrome (PCOS) phlegm‐dampness syndrome, nonphlegm‐dampness normal population. Besides, changes clinical curative effects Jiawei Qi Gong Wan on were evaluated. Patients Methods A total 22 PCOS 21 20 control volunteers selected for this study. The general index, sex hormone fasting blood glucose (FPG), serum insulin (FINS) determined each groups. group...

Abstract Objective The objective of this study was to evaluate the effects triggering strategies on pregnancy outcomes in letrozole-stimulated cycles for frozen-thawed embryo transfer (L-FET), employing gonadotropin-releasing hormone agonist (GnRHa) and human chorionic gonadotropin (hCG) combination, conventional hCG trigger alone GnRHa alone. Materials Methods 773 women undergoing L-FET were enrolled retrospective study. grouping participants based method employed. dual group (N = 105)...

<title>Abstract</title> Long non-coding RNAs (lncRNAs) play an important role in ovarian aging by affecting the biological functions of granulosa cells (GCs) through multiple mechanisms. The lncRNA NEAT1 is significantly downregulated ovaries; however, exact regulatory mechanism has not been elucidated. In current study, we aimed to investigate effects GC during and explore its therapeutic potential. We observed that expression GCs older patients with diminished reserve (DOR), which closely...

Infertility is a reproductive health problem that attracts worldwide attention. Polycystic ovary syndrome (PCOS) major cause of female infertility and patients with obesity PCOS are particularly common in clinical practice. Long non-coding RNA (lncRNAs) functional core cells regulate gene expression, transcription, chromatin modification processes, participate epigenetics, cell cycle, differentiation. LncRNAs assumed to play role the occurrence development PCOS; however, their specific...

Objective : The objective of this study was to investigate the micro-RNA differences between women advanced age with a diminished ovarian reserve (DOR) and young normal (NOR), causes leading decline function oocyte in women, which may be related aging. Methods prospective cohort investigation method used study. We microRNA sequencing detect expression profiles for DOR NOR function. Then, differentially expressed microRNAs were compared agerelated mechanism predicted by target genes. Results...