A5034141205- Cholangiocarcinoma and Gallbladder Cancer Studies
- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- Cancer, Lipids, and Metabolism
- Cancer, Hypoxia, and Metabolism
- Peptidase Inhibition and Analysis
- Immune Cell Function and Interaction
- Ubiquitin and proteasome pathways
- Autophagy in Disease and Therapy
- Metabolomics and Mass Spectrometry Studies
- Cancer Mechanisms and Therapy
- Hepatocellular Carcinoma Treatment and Prognosis
- MicroRNA in disease regulation
- Liver Disease Diagnosis and Treatment
- Genomics, phytochemicals, and oxidative stress
- Hydrogen's biological and therapeutic effects
- Ferroptosis and cancer prognosis
- Nanoplatforms for cancer theranostics
- Microscopic Colitis
- Wnt/β-catenin signaling in development and cancer
- Epigenetics and DNA Methylation
- Antioxidants, Aging, Portulaca oleracea
- Liver Disease and Transplantation
- Cancer-related Molecular Pathways
- Plant Molecular Biology Research
Eastern Hepatobiliary Surgery Hospital
2016-2025
Second Military Medical University
2016-2025
Shanghai Cancer Institute
2013-2025
The University of Melbourne
2025
Xijing Hospital
2024
Shandong University of Traditional Chinese Medicine
2022-2024
Air Force Medical University
2024
University of British Columbia
2022-2023
Affiliated Hospital of Shandong University of Traditional Chinese Medicine
2022-2023
China Medical University
2023
Abstract COVID-19, caused by SARS-CoV-2, has recently affected over 1,200,000 people and killed more than 60,000. The key immune cell subsets change their states during the course of COVID-19 remain unclear. We sought to comprehensively characterize transcriptional changes in peripheral blood mononuclear cells recovery stage single-cell RNA sequencing technique. It was found that T decreased remarkably, whereas monocytes increased patients early (ERS) COVID-19. There an ratio classical CD14...
Src-homology 2 domain-containing phosphatase (Shp2) has been reported to play an important role in the maintenance and self-renewal of embryonic adult stem cells, but its cancer cells (CSCs) remains obscure. Herein, we observed high expression Shp2 both chemoresistant hepatocellular carcinomas (HCCs) recurrent HCCs from patients. A remarkable increase was detected sorted epithelial cell adhesion molecule-positive or cluster differentiation 133-positive liver CSCs CSC-enriched hepatoma...
Solid tumors often suffer from suboptimal oxygen and nutrient supplies. This stress underlies the requirement for metabolic adaptation. Aberrantly activated de novo lipogenesis is critical development progression of human hepatocellular carcinoma (HCC). However, whether influences biological behaviors HCCs under conditions are still poorly understood. Here, we show that display distinct levels glucose‐derived lipogenesis, which positively correlated with their survival responses to glucose...
Hepatocellular carcinoma (HCC) is one of the most lethal malignancies. The lack effective screening methods for early diagnosis has been a longstanding bottleneck to improve survival rate. In present study, capillary electrophoresis–time-of-flight mass spectrometry (CE–TOF/MS)-based metabolomics method was employed discover novel biomarkers HCC. A total 183 human serum specimens (77 sera in discovery set and 106 external validation set) were enrolled this "serum biomarker model" including...
Introduction Cholangiocarcinoma (CCA) is a malignant tumor of the biliary epithelium with poor prognosis. The lack biomarkers to predict therapeutic response and prognosis one major challenges for CCA treatment. Tertiary lymphoid structures (TLS) provide local pivotal microenvironment immune responses. prognostic value clinical relevance TLS in remain unclear. We aimed explore characteristics significance CCA. Methods investigated using surgery cohort containing 471 patients (cohort 1) an...
Abstract Arbuscular mycorrhizal fungi (AMF) that establish reciprocal symbiosis with plant roots can enhance resistance to various stresses, including salt stress, but relevant mechanisms, especially at the molecular level, are scarce. The objective of this study was analyze effect an arbuscular fungus Paraglomus occultum on growth, leaf gas exchange, and expression plasma membrane intrinsic proteins ( PIPs ), tonoplast TIPs ) overly sensitive SOS genes in tomato under (150 mmol/L NaCl)...
Abstract The treatment of hepatocellular carcinoma (HCC) is particularly challenging due to the inherent tumoral heterogeneity and easy resistance towards chemotherapy immunotherapy. Arsenic trioxide (ATO) has emerged as a cytotoxic agent effective for treating solid tumors, including advanced HCC. However, its effectiveness in HCC remains limited, underlying mechanisms are still uncertain. Therefore, this study aimed characterize effects ATO By evaluating susceptibilities human murine cell...
Abstract Aims/hypothesis Type 2 diabetes is a chronic metabolic disorder characterised by insulin resistance and sustained hyperglycaemia, major cause of blindness, kidney failure, heart attacks stroke. Our team has recently identified hexosaminidase A (HEXA) as an endocrine factor secreted the liver that regulates sphingolipid metabolism in skeletal muscle. Specifically, HEXA converts GM2 to GM3 gangliosides within cell-surface lipid rafts. Remodelling ganglioside composition enhances IGF1...
Activation of the mitogen-activated protein kinases (MAPKs) and nuclear factor κB (NF-κB) cascades after Toll-like receptor (TLR) stimulation contributes to innate immune responses. Signal regulatory (SIRP) α, a member SIRP family that is abundantly expressed in macrophages, has been implicated regulating MAPK NF-κB signaling pathways. In addition, SIRPα can negatively regulate phagocytosis host cells by indicating an inhibitory role immunity. We provide evidences essential endogenous...
The drought-tolerant 'Ningchun 47' (NC47) and drought-sensitive 'Chinese Spring' (CS) wheat (Triticum aestivum L.) cultivars were treated with different PEG6000 concentrations at the three-leaf stage. An analysis on physiological proteomic changes of seedling in response to drought stress was performed. In total, 146 differentially accumulated protein (DAP) spots separated recognised using two-dimensional gel electrophoresis. 101 DAP representing 77 unique proteins identified by...
Asparagine synthetase (ASNS) is associated with drug resistance in leukaemia, and the function of this enzyme context hepatocellular carcinoma (HCC) not clear. In study, relationship between ASNS expression clinical outcomes after surgical resection was investigated, therapeutic value also evaluated. The evaluated HCC samples by real-time PCR immunohistochemistry assays. correlation clinicopathological features investigated. Potential prognostic factors were examined univariate multivariate...
Autophagy enables cells to recycle long-lived proteins or damaged organelles. Beclin 1 plays important roles in autophagy, differentiation, apoptosis and the development progression of cancer, but expression its possible role primary intrahepatic cholangiocarcinoma (ICC) has not been reported yet. This study aimed investigate prognostic significance ICC. First, we assessed levels Becn1 by real-time PCR 50 ICC samples found mRNA was markedly increased 78% (39 50) compared with normal bile...
CD133/Prominin-1 is a pentaspan transmembrane protein that has been frequently used as biomarker for cancer stem cells, although its biological function unclear. The aim of our study was to explore the intrinsic functions CD133 membrane in hepatoma cells during autophagy, apoptosis, tumorigenesis and cell survival through expression or downregulation CD133. In this study, found be dynamically released from plasma into cytoplasm both complete medium(CM) low glucose medium (LGM), LGM promoted...
A robust, reproducible, and high throughput method was developed for the relative quantitative analysis of glycoprotein abundances in human serum. Instead quantifying glycoproteins by glycopeptides conventional glycoproteomics, were quantified nonglycosylated peptides derived from digest, which consists capture serum samples release nonglycopeptides trypsin digestion captured followed two-dimensional liquid chromatography-tandem MS released peptides. Protein quantification achieved comparing...
Aberrant expression of A20 has been reported in several human malignancies including hepatocellular carcinoma (HCC). However, its clinical relevance and potential role HCC remain unknown. Quantitative PCR, Western blots immunohistochemistry analyses were used to quantify samples cell lines. The correlation with clinicopathologic features was analyzed a cohort containing 143 patients primary HCC. Kaplan-Meier curves evaluate the association between patient survival. Functional studies...
Deregulation of the immune system is believed to contribute cancer malignancy, which has led recent therapeutic breakthroughs facilitating antitumor immunity. In a malignant setting, immunoglobulin receptors, are fundamental components human system, fulfill paradoxical roles in pathogenesis. This study describes previously unrecognized pro‐oncogenic function polymeric receptor (pIgR) promotion cell transformation and proliferation. Mechanistically, pIgR overexpression associated with YES...
Oxidative stress status has a key role in hepatocellular carcinoma (HCC) development and progression. Normally, reactive oxygen species (ROS) levels are tightly controlled by an inducible antioxidant program that responds to cellular stressors. How HCC cells respond excessive oxidative remains elusive. Here, we identified feedback loop between gankyrin, oncoprotein overexpressed human HCC, Nrf2 maintaining the homeostasis cells. Mechanistically, gankyrin was found interact with Kelch domain...
Gemcitabine is a nucleoside analog that has been successfully used in the treatment of multiple cancers. However, intrinsic or acquired resistance reduces chemotherapeutic potential gemcitabine. Here, we revealed previously unappreciated mechanism by which phosphatase and tensin homolog (PTEN), one most frequently mutated genes human cancers, dominates decision-making process central to regulation gemcitabine efficacy cholangiocarcinoma (CCA). By investigating gemcitabine-treated CCA cohort,...