A5100723768- Lung Cancer Research Studies
- RNA modifications and cancer
- Epigenetics and DNA Methylation
- Chromatin Remodeling and Cancer
- Blood Coagulation and Thrombosis Mechanisms
- Neuroendocrine Tumor Research Advances
- Peptidase Inhibition and Analysis
- Cancer, Lipids, and Metabolism
- Cancer Cells and Metastasis
- Cancer, Hypoxia, and Metabolism
- Angiogenesis and VEGF in Cancer
- Ubiquitin and proteasome pathways
- Cancer therapeutics and mechanisms
- Hippo pathway signaling and YAP/TAZ
- Cancer-related molecular mechanisms research
- Cancer Genomics and Diagnostics
- Cancer Mechanisms and Therapy
- Cell Adhesion Molecules Research
- Cancer-related gene regulation
- Genetic factors in colorectal cancer
- Plant Molecular Biology Research
- ATP Synthase and ATPases Research
- Ferroptosis and cancer prognosis
- HER2/EGFR in Cancer Research
- Microtubule and mitosis dynamics
Center for Excellence in Molecular Cell Science
2019-2025
Chinese Academy of Sciences
2012-2025
NYU Langone Health
2024
University of Chinese Academy of Sciences
2016-2023
State Key Laboratory of Cell Biology
2021
SAIC-GM (China)
2021
Nanjing Medical University
2020
Weatherford College
2020
Shanghai Institutes for Biological Sciences
2019
Nanjing University of Chinese Medicine
2019
Alterations of gut microbiota have been implicated in multiple diseases including cancer. However, the spectrum lung cancer remains largely unknown. Here we profiled composition a discovery cohort containing 42 early-stage patients and 65 healthy individuals through 16S ribosomal RNA (rRNA) gene sequencing analysis. We found that displayed significant shift contrast to populations. To identify optimal signature for noninvasive diagnosis purpose, took advantage Support-Vector Machine (SVM)...
KRASG12C inhibitors (adagrasib and sotorasib) have shown clinical promise in targeting KRASG12C-mutated lung cancers; however, most patients eventually develop resistance. In with adenocarcinoma STK11/LKB1 co-mutations, we find an enrichment of the squamous cell carcinoma gene signature pre-treatment biopsies correlates a poor response to adagrasib. Studies Lkb1-deficient KrasG12D cancer mouse models organoids treated KRAS reveal tumors invoke lineage plasticity program, adeno-to-squamous...
Abstract Lkb1 deficiency confers the Kras -mutant lung cancer with strong plasticity and potential for adeno-to-squamous transdifferentiation (AST). However, it remains largely unknown how dynamically regulates AST. Using classical AST mouse model ( LSL-G12D/+ ;Lkb1 flox/flox , KL ), we here comprehensively analyze temporal transcriptomic dynamics of tumors at different stages by dynamic network biomarker (DNB) identify tipping point which Wnt signaling is abruptly suppressed excessive...
Abstract Cathepsin D (CD) up-regulation has been associated with human malignancy and poor prognosis. Thrombin up-regulated CD mRNA protein in eight tumor cell lines as well umbilical vascular endothelial cells (HUVEC). increased the secretion of by 3- to 8-fold enhanced chemotaxis (∼2-fold) 4T1 murine mammary CA cells, which was completely inhibited knockdown CD. Secreted induced neoangiogenesis 2.4-fold on a chick chorioallantoic membrane, blocked CD-KD cells. The addition pure (2 ng)...
Drug resistance is a significant hindrance to effective cancer treatment. Although mechanisms of epidermal growth factor receptor (EGFR) mutant cells lethal EGFR tyrosine kinase inhibitors (TKI) treatment have been investigated intensively, how orchestrate adaptive response under sublethal drug challenge remains largely unknown. Here, we find that 2-h TKI elicits transient drug-tolerant state in lung cells. Continuous reinforces this tolerance and eventually establishes long-term resistance....
Metastasis is the dominant cause of patient death in small-cell lung cancer (SCLC), and a better understanding molecular mechanisms underlying SCLC metastasis may potentially improve clinical treatment. Through genome-scale screening for key regulators mouse Rb1-/- Trp53-/- using pooled CRISPR/Cas9 library, we identified Cullin5 (CUL5) suppressor cytokine signaling 3 (SOCS3), two components Cullin-RING E3 ubiquitin ligase complex, as top candidates. Mechanistically, deficiency CUL5 or SOCS3...
Human lung adenosquamous cell carcinoma (LUAS), containing both adenomatous and squamous pathologies, harbors strong plasticity is significantly associated with poor prognosis. We established an up-to-date comprehensive genomic transcriptomic landscape of LUAS in 109 Chinese specimens demonstrated development via adeno-to-squamous transdifferentiation. Unsupervised clustering dynamic network biomarker analysis identified inflammatory subtype as the critical transition stage during...
Abstract The mechanism of thrombin-induced angiogenesis is poorly understood. Using a gene chip array to investigate the pro-malignant phenotype thrombin-stimulated cells, we observed that thrombin markedly up-regulates growth-regulated oncogene-α (GRO-α) in several tumor cell lines as well endothelial cells by mRNA and protein analysis. Thrombin enhanced secretion GRO-α from 25- 64-fold. CXC chemokine with tumor-associated angiogenic oncogenic activation following ligation its CXCR2...
Aberrant expression of A20 has been reported in several human malignancies including hepatocellular carcinoma (HCC). However, its clinical relevance and potential role HCC remain unknown. Quantitative PCR, Western blots immunohistochemistry analyses were used to quantify samples cell lines. The correlation with clinicopathologic features was analyzed a cohort containing 143 patients primary HCC. Kaplan-Meier curves evaluate the association between patient survival. Functional studies...
Human lung adenosquamous cell carcinoma (LUAS), containing both adenomatous and squamous pathologies, exhibits strong cancer plasticity. We find that ALK rearrangement is detectable in 5.1–7.5% of human LUAS, transgenic expression EML4-ALK drives adenocarcinoma (LUAD) formation initially transition at late stage. identify club cells as the main cell-of-origin for transition. Through recapitulating lineage organoid system, we JAK-STAT signaling, activated by phase separation, significantly...
Phenotypic plasticity is a rising cancer hallmark, and lung adeno-to-squamous transition (AST) triggered by LKB1 inactivation significantly associated with drug resistance. Mechanistic insights into AST are urgently needed to identify therapeutic vulnerability in LKB1-deficient cancer. Here, we find that ten-eleven translocation (TET)–mediated DNA demethylation elevated during KrasLSL-G12D/+; Lkb1L/L (KL) mice, knockout of individual Tet genes reveals Tet2 required for squamous transition....
Antiplatelet GPIIIa49–66 Ab of HIV-related thrombocytopenic patients induces thrombocytopenia and platelet fragmentation by the generation peroxide other reactive oxygen species (ROS). Here we report presence a functional NADPH oxidase pathway that requires activation 12-lipoxygenase (12-LO) to fragment platelets. A new Ab-mediated mechanism is described in which 12-LO product, 12(S)-HETE activates generate ROS.
Abstract Kv2.1 as a voltage-gated potassium (Kv) channel subunit has pivotal role in the regulation of glucose-stimulated insulin secretion (GSIS) and pancreatic β -cell apoptosis, is believed to be promising target for anti-diabetic drug discovery, although mechanism underlying Kv2.1-mediated apoptosis obscure. Here, small molecular compound, ethyl 5-(3-ethoxy-4-methoxyphenyl)-2-(4-hydroxy-3-methoxybenzylidene)-7-methyl-3-oxo-2,3-dihydro-5H-[1,3]thiazolo[3,2–a]pyrimidine-6-carboxylate...
Aberrant expression of choline kinase alpha (CHKA) has been reported in a variety human malignancies including colorectal carcinoma (CRC). However, the role CHKA progression and prognosis CRC remains unknown. In this study, we found that was frequently upregulated clinical samples CRC-derived cell lines significantly correlated with lymph node metastasis (p = 0.028), TNM stage 0.009), disease recurrence 0.004) death < 0.001). Survival analyses indicated patients higher had shorter...
Chromobox 4 (CBX4) is a core component of polycomb-repressive complex 1 with important roles in cancer biology and tissue homeostasis. Aberrant expression CBX4 has been implicated several human malignancies. However, its role underlying mechanisms the tumorigenesis lung adenocarcinoma (LUAD) have not defined vivo. Here, we found that was frequently up-regulated LUAD samples correlated poor patient survival. Importantly, genetic ablation greatly dampened tumor formation improved survival...
Carbonic anhydrases (CAs) have been implicated in the pathogenesis of human cancers. anhydrase VII (CA7), a member CA gene family, was recently demonstrated to be expressed several tissues including colon. Nevertheless, expression and clinical relevance CA7 colorectal carcinoma (CRC) has not investigated.Real-time PCR, western blot, immunohistochemistry analyses were used determine CRC samples. The correlation with clinicopathologic features assessed 228 patients from Luoyang, China...