Angiogenesis is initiated in response to a variety of external cues, including mechanical and biochemical stimuli; however, the underlying signaling mechanisms remain unclear. Here, we investigated proangiogenic role endothelial mechanosensor Piezo1. Genetic deletion pharmacological inhibition Piezo1 reduced sprouting lumen formation induced by wall shear stress mediator sphingosine 1-phosphate, whereas activation selective activator Yoda1 enhanced angiogenesis. Similarly stress, 1-phosphate...

The molecular mechanisms underlying the regulation of pluripotency by cellular metabolism in human embryonic stem cells (hESCs) are not fully understood. We found that high levels glutamine essential to prevent degradation OCT4, a key transcription factor regulating hESC pluripotency. Glutamine withdrawal depletes endogenous antioxidant glutathione (GSH), which results oxidation OCT4 cysteine residues required for its DNA binding and enhanced degradation. emergence OCT4(lo) cell population...

Endothelial cells normally line the vasculature and remain quiescent. However, these can be rapidly stimulated to undergo morphogenesis initiate new blood vessel formation given proper cues. This study reports a mechanism for initiating angiogenic sprout that involves vimentin, major intermediate filament protein in endothelial cells. Initial studies confirmed vimentin was required sphingosine 1-phosphate (S1P)- growth factor (GF)-induced cell invasion, cleaved by calpains during invasion....

Disruption of alveolar-capillary barriers is a major complication high-volume mechanical ventilation referred to as "ventilator-induced lung injury." The stretching force in alveoli transmitted endothelial cells, increasing the tension on underlying plasma membrane. mechanosensor Piezo1, membrane cation channel, was inducibly deleted cells mice (Piezo1iEC-/-), which allowed us study its role regulating barrier response alveolar stretch. We observed significant increase vascular permeability...

Endothelial cells are subjected to biochemical and mechanical stimuli, which regulate their angiogenic potential. We determined the synergistic effects of sphingosine-1-phosphate (S1P) fluid wall shear stress (WSS) on a previously established model human umbilical vein endothelial cell invasion into three-dimensional collagen matrices. Collagen matrices were incorporated parallel-plate flow chamber apply controlled WSS surface monolayers over period 24 h. Cell required presence S1P, with S1P...

본 연구는 고분자 당을 이용하여 탈수하는 방법인 분자압축탈수건조법으로 처리된 인삼을 분말화하여 쿠키에 첨가한 기능성 인삼 쿠키를 개발해보고자 한 것으로 그 품질특성을 살펴본 것이다. 연구 결과, 쿠키의 색도는 인삼분말 첨가량이 증가할수록 명도는 감소하고, 적색도는 증가했다. 퍼짐성은 인삼분말을 첨가하지 않은 대조군이 가장 컸으며 분말 유의적으로 작아짐을 알 수 있었다. 경도는 대조군과 1% 유의적인 차이가 없었으나 분말을 첨가할수록 경도가 증가하는 것을 보였다. 관능검사를 실시한 결과 외관은 쿠키가 높은 점수를 나타내었고 명도가 감소하고 적색도가 증가한 보아 이러한 현상이 외관의 기호도에 영향을 미친 생각된다. 향의 기호도는 모든 시료에서 없었다. 맛의 1, 3% 첨가군 간에 없었고 5% 첨가시 낮은 값을 단맛은 쿠키에서 받았다. 조직감은 높았고 1%, 순으로 낮게 나타났다. 전반적인 1%를 7.50으로 높았고, 대조군, 3%, 첨가하여 제조한 품질특성 색, 퍼짐성, 조직감...

Podosomes are compartmentalized actin-rich adhesions, defined by their ability to locally secrete proteases and remodel extracellular matrix. Matrix remodeling endothelial podosomes facilitates invasion thereby vessel formation. However, the mechanisms underlying podosome formation function remain unclear. Here, we demonstrate that Septin2, Septin6, Septin7 required for maturation of nascent into matrix-degrading organelles. We show development occurs through initial mobilization scaffolding...

즉석 백설기 제조 시 인삼분말 첨가가 품질 특성에 미치는 영향을 살펴본 결과 저장 24시간째에 수분함량이 인삼분말을 첨가하지 않았을 때는 감소한 반면, 첨가한 처리군에서는 수분함량을 초기 그대로 유지시켜 주는 것으로 나타났다. 색도는 인삼 분말을 첨가함에 따라 L값은 낮아지고, a, b값은 점점 증가하는 조직감에서는 0, hardness와 gumminess, chewiness가 가장 큰 유의적인 차이를 보였으나, 2%, 3% 첨가했을 모든 군에서 보이지 않았다. 마지막으로 관능평가 경우 수분을 보유하여 주기 때문에 않은 경우보다 촉촉함과 전반적인 기호도에서 높은 점수를 받았다. 이로 인해 첨가하였을 때 주고, 색과 조직감을 변하지 않게 해 준다는 것을 본 연구를 통해 확인할 수 있었다. 결론적으로 백설기의 있었고, 떡의 품질을 나타내는 척도인 hardness의 변화를 적게 하여 첨가군이 무첨가군에 비해 노화 억제에 효과가 있는 알 첨가는 수분, 색, 유지시켜주는 확인되었고...

Angiogenesis, the formation of new blood vessels from pre-existing structures, is a key step in development, wound healing and pathological events (1). ECs vascular system must continually sense respond to both biochemical mechanical stimuli appropriately initiate angiogenesis. Therefore, studying combined influences fluid wall shear stress (WSS) pro-angiogenic factors on 3D matrices critical precisely define signals that sprouting angiogenesis, where normally quiescent transition into an...

New blood vessel growth is essential for vascularization of wounded tissues. Endothelial cells are integral this process and stimulated by factors platelet‐derived lipids such as sphingosine‐1‐phosphate (S1P), while also integrating mechanical signals, wall shear stress (WSS). However the molecular signals activated downstream these cues not completely defined. Previously, we have established a system to demonstrate that S1P WSS combine stimulate primary endothelial cell invasion 3D collagen...

Endothelial cells are subjected to biochemical and mechanical stimuli which regulate their angiogenic potential. We determined the synergistic effects of sphingosine-1-phosphate (S1P) fluid shear wall stress (WSS) on invasion human umbilical vein endothelial (HUVECs) into three-dimensional collagen matrices. A gel was incorporated a parallel-plate flow chamber apply controlled WSS surface HUVEC monolayers over period 24hr. Cell gels required presence S1P, with S1P being enhanced by an extent...

Vascular endothelial cell (EC) sensing of wall shear stress (WSS) in areas disturbed hemodynamics induces severe neovascularization; however, the underlying mechanisms remain unclear. Here we determined role Piezo1, WSS mechanosensor localized luminal aspect ECs, mechanism angiogenesis. We demonstrate that activation Src kinase response to angiogenic mediators such as S1P and VEGF phosphorylation Piezo1 at Y1165. This Src-activated phospho-switch turn gates Ca2+ via signal vessel sprouting...

We have previously developed a cell culture model to study the effects of angiogenic factors, such as sphingosine-1-phosphate (S1P), on invasion endothelial cells into underlying extracellular matrix. In addition biochemical stimuli, vascular are subjected fluid shear stress due blood flow. The present is aimed at determining collagen gels. A device was constructed apply well-defined stresses confluent human umbilical vein (HUVECs) seeded Fluid induced significant increases in with maximal...

In the initial phases of angiogenesis endothelial cells must degrade extracellular matrix before migrating to alternative sites form nascent vessels. The degradation is performed by podosomes, actin rich structures that are regulated Src family kinases. Here we report filamentous GTPase Septin2 phosphorylated upon activation and plays a critical role in Src‐mediated podosome formation degradation. We have identified induces phosphorylation its translocation cells. Super resolution microscopy...

The sensing of wall shear stress (WSS) by endothelial cells (ECs) mediates sprouting angiogenesis; however, the underlying signaling mechanisms remain unclear. Here we investigated synergism between WSS and angiogenic mediator Sphingosine‐1‐phosphate (S1P) in activation mechanosensitive ion channel protein Piezo1, expressed ECs. Studies were made EC monolayers exposed to 5 dyne/cm 2 , level approximating venular WSS, which angiogenesis 3D collagen matrix was assessed with without challenge...

Endothelial cells (ECs) must respond rapidly to a variety of extracellular cues initiate sprouting events. We undertook the current study better understand how mechanical forces (shear stress) combine with biochemical promote sprout outgrowth in defined 3D model EC invasion, because while effects shear stress have been widely studied atherosclerosis, molecular events directed by angiogenic are incompletely understood. initially focused on known mechanosensitive protein Filamin A, which has...

Sphingosine‐1‐phosphate receptor 1 (S1PR1) is critical regulator of endothelial cell (EC) migration, cytoskeletal structure, capillary‐network formation, sprouting angiogenesis, and vascular maturation. Here we developed a strategy for controlling barrier function angiogenesis using an engineered photo activatable (PA)‐S1PR1 receptor. Regulation signaling by PA‐S1PR1 was demonstrated in human pulmonary arterial cells (HPAECs). Following illumination with blue light, induced activation known...

ERG, a member of ETS family transcription factors, acts as key modulator endothelial cell differentiation and haematopoiesis. Despite the high degree expression ERG in cells, its role maintaining adult vascular architecture remain elusive. We show that is expressed at highest level lung than other vascularized organs such kidney brain. Intriguingly, we found conditional deletion cells CDh5‐Cre‐ERT mice (ERG i Δ EC ) spontaneously compromised vessel density these lungs showed severe...