A5007804430- Genetics, Aging, and Longevity in Model Organisms
- DNA Repair Mechanisms
- Heavy Metal Exposure and Toxicity
- Effects and risks of endocrine disrupting chemicals
- Carcinogens and Genotoxicity Assessment
- Nanoparticles: synthesis and applications
- Toxic Organic Pollutants Impact
- Reproductive Biology and Fertility
- Microplastics and Plastic Pollution
- Advanced Chemical Sensor Technologies
- Water Treatment and Disinfection
- Antimicrobial agents and applications
- Spaceflight effects on biology
- Adipose Tissue and Metabolism
- CRISPR and Genetic Engineering
- Insect and Pesticide Research
- Environmental Chemistry and Analysis
- FOXO transcription factor regulation
- Trace Elements in Health
- Signaling Pathways in Disease
- Cancer Mechanisms and Therapy
- Reproductive System and Pregnancy
- Analytical Methods in Pharmaceuticals
- Bone Metabolism and Diseases
- Connective tissue disorders research
Korea Institute of Toxicology
2020-2025
Virginia Commonwealth University
2015-2021
University of Ulsan
2004-2015
Institute on Aging
2008
National Institute on Aging
2008
National Institutes of Health
2008
Stress depletes STAT3 in the mitochondria, and restoration of this pool suppresses stress-induced ROS production.
Bisphenol A (BPA) is a chemical compound commonly used in the production of plastics for daily lives and industry. As BPA well known its adverse health effects, several alternative materials have been developed. This study comprehensively analyzed toxicity three substitutes including bisphenol S (BPS), F (BPF), tetramethyl (TMBPF) on aging, healthspan, mitochondria using an vivo Caenorhabditis elegans (C. elegans) model animal cultured mammalian fibroblast cells. C. treated with 1 mM...
Abstract Melatonin protects against Cadmium (Cd)-induced toxicity, a ubiquitous environmental toxicant that causes adverse health effects by increasing reactive oxygen species (ROS) production and mitochondrial dysfunction. However, the underlying mechanism remains unclear. Here, we demonstrate Cd exposure reduces levels of mitochondrially-localized signal transducer activator transcription 3 (mitoSTAT3) using human prostate stromal cells mouse embryonic fibroblasts. enhances mitoSTAT3...
DNA repair is an important mechanism by which cells maintain genomic integrity. Decline in capacity or defects factors are thought to contribute premature aging mammals. The nematode Caenorhabditis elegans a good model for studying longevity and because of key advances understanding the genetics this organism. Long-lived C. mutants have been identified shown be resistant oxidizing agents UV irradiation, suggesting genetically determined correlation between life span. In report, gene-specific...
WRN-1 is the Caenorhabditis elegans homolog of human Werner syndrome protein, a RecQ helicase, mutations which are associated with premature aging and increased genome instability. Relatively little known as to how functions in DNA repair damage signaling. Here, we take advantage genetic cytological approaches C. dissect epistatic relationship various checkpoint pathways. We found that required for CHK1 phosphorylation induced by replication inhibition, but not UV radiation. Furthermore,...
Abstract Animals change feeding behavior depending on their metabolic status; starved animals are eager to eat and satiated stop eating. C. elegans exhibits satiety quiescence under certain conditions that mimics many aspects of post-prandial sleep in mammals. Here we show this depends fat metabolism mediated by the SREBP-SCD pathway, an acetyl-CoA carboxylase (ACC) nuclear hormone receptors (NRs). Mutations genes pathway reduce quiescence. An RNA interference (RNAi) screen regulate glucose...
N-(1,3-Dimethylbutyl)-N'-phenyl-p-phenylenediamine (6PPD), a synthetic additive widely used in the rubber industry, and its oxidized product 6PPD-quinone (6PPDQ), have garnered widespread attention as an emerging hazardous chemicals owing to their potential detrimental effects on aquatic ecosystem human health. The of 6PPD 6PPDq female reproductive tract, especially embryo implantation, remain unknown were investigated this study. We spheroid attachment outgrowth models BeWo trophoblastic...
Abstract All females adopt an evolutionary conserved reproduction strategy; under unfavorable conditions such as scarcity of food or mates, oocytes remain quiescent. However, the signals to maintain oocyte quiescence are largely unknown. Here, we report that in four different species – Caenorhabditis elegans , remanei Drosophila melanogaster and Danio rerio octopamine norepinephrine play essential role maintaining quiescence. In absence mutants lacking signaling fail quiescent, but continue...
Environmental stress triggers multilevel adaptations in animal development that depend part on epigenetic mechanisms. In response to harsh environmental conditions and pheromone signals, Caenorhabditis elegans larvae become the highly stress-resistant long-lived dauer. Despite extensive studies of dauer formation pathways integrate specific cues appear transcriptional reprogramming, role regulation has remained unclear. Here we report BLMP-1, BLIMP-1 ortholog, regulates via pathways; absence...
Osteoporosis is a major public health concern, which requires novel therapeutic strategies to prevent or mitigate bone loss.Natural compounds have attracted attention as potential agents due their safety and efficacy.In this study, we investigated the regulatory activities of boeravinone B (BOB), natural rotenoid isolated from medicinal plant Boerhavia diffusa, on differentiation osteoclasts mesenchymal stem cells (MSCs), two main cell components responsible for remodeling.We found that BOB...
The highly conserved RecQ helicases are essential for the maintenance of genomic stability. Werner syndrome protein, WRN, is one five human helicase homologues, and a deficiency protein causes hereditary premature aging disorder that characterized by instability. A WRN orthologue, wrn-1 lacking exonuclease domain, has been identified in nematode Caenorhabditis elegans. wrn-1(RNAi) C. elegans shortened life span, increased sensitivity to DNA damage, accelerated phenotypes. However, little...
The Caenorhabditis elegans Werner syndrome protein, WRN-1, a member of the RecQ helicase family, has 3'-5' DNA activity. Worms with defective wrn-1 exhibit premature aging phenotypes and an increased level genome instability. In response to damage, WRN-1 participates in initial stages checkpoint activation concert C. replication protein A (RPA-1). is stimulated by RPA-1 on long duplex substrates. However, mechanism which stimulates unwinding function WRN-1-RPA-1 interaction are not clearly...
Cadmium (Cd), a harmful heavy metal, can lead to various pulmonary diseases, including chronic obstructive disease (COPD), by inducing cytotoxicity and disturbing redox homeostasis. The aim of the present study was investigate Cd-mediated using human lung fibroblasts therapeutic potential 3,3'-diindolylmethane (DIM). significantly reduced cell viability embryonic (HEL299) cells accompanied enhanced oxidative stress as evidenced increased expression autophagy-related proteins such LC3B p62....
Abstract Background Pectolinarigenin (PEC) is a flavone extracted from Cirsium , and because it has anti-inflammatory properties, anti-cancer research also being conducted. The objective of this work was to find out if PEC involved in tumor control which pathways regulates vivo vitro. Methods AGS cell lines were xenografted into BALB/c nude mice create tumors, administered intraperitoneally see control. Once animal testing completed, proteins isolated identified using LC–MS analysis, gene...
Bisphenol AF (BPAF) is found in high concentrations aquatic environments due to the increased use of thermal paper and food packaging. However, there have been relatively few toxicological studies potential risk assessments BPAF. In this study, quotient (RQ) hazard (HQ) BPAF were derived present safety standards for environmental management protection lakes, rivers, bays, Italian regions. We applied species sensitivity distribution (SSD) method based on previous ecotoxicological data results...
Abstract mua-3 is a Caenorhabditis elegans homolog of the mammalian fibrillin1, monogenic cause Marfan syndrome. We identified new mutation that carries an in-frame deletion 131 amino acids in extracellular domain, which allows mutants to survive temperature-dependent manner; at permissive temperature, grow normally without obvious phenotypes, but nonpermissive more than 90% die during L4 molt due internal organ detachment. Using temperature-sensitive lethality, we performed unbiased genetic...
The toxicity of cadmium (Cd) occurs through accumulation in the environment. precise mechanism underlying Cd remains unclear. Therefore, present study, we studied effects on MM55.K cells and investigated mechanisms Cd-induced cell death. CdCl2 significantly elevated apoptotic death, mitochondrial membrane potential (ΔΨm) loss, caspase-dependent Moreover, immunoblotting results revealed that down-regulated inhibitor protein such as survivin Bcl-2 which led to activation caspase-3 cleavage...