A5084672605- Plant Physiology and Cultivation Studies
- Plant Gene Expression Analysis
- GABA and Rice Research
- Plant Disease Resistance and Genetics
- Genetic Mapping and Diversity in Plants and Animals
- Cocoa and Sweet Potato Agronomy
- Chromosomal and Genetic Variations
- Legume Nitrogen Fixing Symbiosis
- Genomics and Phylogenetic Studies
- Plant Molecular Biology Research
- Plant Stress Responses and Tolerance
- Genetic and Environmental Crop Studies
- Rice Cultivation and Yield Improvement
- Agricultural pest management studies
- Photosynthetic Processes and Mechanisms
- Phytoplasmas and Hemiptera pathogens
- Plant Pathogens and Fungal Diseases
- Plant Virus Research Studies
- Plant-Microbe Interactions and Immunity
- Bacillus and Francisella bacterial research
- 14-3-3 protein interactions
- Microbial Metabolism and Applications
- Plant Pathogenic Bacteria Studies
- Plant nutrient uptake and metabolism
- Soybean genetics and cultivation
National Research Centre on Plant Biotechnology
2011-2023
Indian Council of Agricultural Research
2023
Indian Agricultural Research Institute
2011-2018
Banasthali University
2017
Pigeonpea (Cajanus cajan) is an important grain legume of the Indian subcontinent, South-East Asia and East Africa. More than eighty five percent world pigeonpea produced consumed in India where it a key crop for food nutritional security people. Here we present first draft genome sequence popular variety ‘Asha’. The was assembled using long reads 454 GS-FLX sequencing chemistry with mean read lengths >550 bp >10-fold coverage, resulting 510,809,477 high quality sequence. Total 47,004...
Abstract Single nucleotide polymorphism (SNP) is the most abundant DNA sequence variation present in plant genomes. Here, we report design and validation of a unique genic-SNP genotyping chip for genetic evolutionary studies as well molecular breeding applications rice. The incorporates 50,051 SNPs from 18,980 different genes spanning 12 rice chromosomes, including 3,710 single-copy (SC) conserved between wheat rice, 14,959 SC to 194 agronomically important cloned 117 multi-copy genes....
Increased water and labour scarcity in major rice growing areas warrants a shift towards direct seeded cultivation under which management of weeds is issue. Use broad spectrum non-selective herbicides an efficient means to manage weeds. Availability genotypes with complete tolerance against broad-spectrum pre-requisite for advocating use such herbicides. In the present study, we developed EMS induced mutant, 'HTM-N22', exhibiting herbicide, 'Imazethapyr', identified mutations imparting...
Here, we present the draft genome (377.3 Mbp) of Corchorus olitorious cv. JRO-524 (Navin), which is a leading dark jute variety developed from cross between African (cv. Sudan Green) and indigenous JRO-632) types. We predicted total 57,087 protein-coding genes with annotated functions. identified large number 1765 disease resistance-like defense response in genome. The showed highest sequence similarities that Theobroma cacao followed by Gossypium raimondii. Seven chromosome-scale...
Pigeonpea is the second most important pulse legume crop for food and nutritional security of South Asia that requires accelerated breeding using high throughput genomic tools. Single nucleotide polymorphisms (SNPs) are highly suitable markers this purpose because their bi-allelic nature, reproducibility abundance in genome. Here we report on development use a pigeonpea 62 K SNP chip array 'CcSNPnks' Affymetrix GeneTitan® platform. The was designed after filtering 645,662 genic-SNPs...
Mango is one of the most important fruits tropical ecological region world, well known for its nutritive value, aroma and taste. Its world production >45MT worth >200 billion US dollars. Genomic resources are required improvement in productivity management mango germplasm. There no web-based genomic available mango. Hence rapid cost-effective high throughput putative marker discovery to develop such resources. RAD-based can cater this urgent need till whole genome sequence becomes available....
Abstract Erratic rainfall leading to flash flooding causes huge yield losses in lowland rice. The traditional varieties and landraces of rice possess variable levels tolerance submergence stress, but gene discovery utilization these resources has been limited the Sub1A-1 allele from variety FR13A. Therefore, we analysed allelic sequence variation three Sub1 genes a panel 179 genotypes its association with tolerance. Population structure diversity analysis based on 36-plex genome wide...
Mango (Mangifera indica L.), acclaimed as the 'king of fruits' in tropical world, has historical, religious, and economic values. It is grown commercially more than 100 countries, fresh mango world trade accounts for ~3,200 million US dollars year 2020. widely cultivated sub-tropical regions with India, China, Thailand being top three producers. fruit adored its taste, color, flavor, aroma. Fruit color firmness are important quality traits consumer acceptance, but their genetics poorly...
Rice is the most important food crop both in value and volume for Asian population. Frequent drought, flood salinity stresses exacerbated by global climate change adversely affect rice production more than fifty percent of growing areas. Green revolution high yielding varieties carrying sd1 dwarfing gene have almost fully replaced traditional resilient landraces rice. However, these were bred primarily yield under input conditions therefore are sensitive to adverse climatic conditions....
Effect of SNAC1 gene variants on drought tolerance was investigated in wild rice accessions. A total 46 INDELs and 44 SNPs were identified including 17 the coding region across 164 Indian accessions four cultivated genotypes. Based sequence variation, 25 haplotypes specific to tolerant Translation different allele sequences showed existence five protein variants. Evolutionary study basis nucleotide variation with neutrality test suggested purifying selection Association analysis revealed...
Whole genome sequence in mango offers unprecedented opportunities for genomics assisted crop improvement via enabling access to genome-wide genetic markers. In the present study, simple repeats (SSRs) were predicted from of mango. Based on SSR length (? 50 bp), highly-variable SSRs (MSSRs) sorted. A sub-set 129 MSSRs was validated a set 24 diverse genotypes yielding marker validation and polymorphism 88.96 85.27 per cent, respectively. One hundred ten polymorphic markers identified...
Rapid advances in DNA sequencing technologies have resulted the accumulation of large data sets public domain, facilitating comparative studies to provide novel insights into evolution life. Phylogenetic across eukaryotic taxa been reported but on basis a limited number genes. Here we present genome-wide analysis different plant, fungal, protist, and animal species, with reference 36,002 expressed genes rice genome. Our revealed 9831 unique 98 conserved all 49 species analysed. The diverse...
Actin and tubulin are cytoskeleton proteins, which important components of the celland conserved across species. Despite their crucial significance in cell motility division distribution phylogeny actin genes taxa is poorly understood. Here we used publicly available genomic data 49 model species plants, animals, fungi Protista for further understanding these among diverse eukaryotic using rice as reference. The highest numbers gene homologs were present plants followed by species, whereas...
Cathepsin B like cysteine proteases representing a major component of the lysosomal proteolytic repertoire plays an important role in intracellular protein degradation. Comparative models cathepsin (CatB) six different Leishmania strains were developed using MODELLER. The modeled three-dimensional (3-D) structure has correct stereochemistry as gauged from Ramachandran plot and good 3-D compatibility assessed by PROCHECK DOPE score ( DS2.1, Accelrys ). T he proteins energy minimized validated...
WRKY proteins play crucial roles in plant defense regulatory networks, development process and physiological programs including responses to several biotic abiotic stresses. Evalutionary analysis revealed, genes were categorized into the four major groups. In developed phylogenetic tree, group-D contain highest number (15) of followed by group-B (10), group-A (7), group-C (6). Several CRE’s identified from mango transcriptome belonging different categories like light responsiveness,...
Polianthes tuberosa is commercially popular because of their economic importance in floriculture for cut and loose flowers perfume industry the unique fragrance. Despite its commercial importance, no ready-to-use transcript sequence information available public database. We have sequenced RNA obtained from tuberose using Illumina HiSeq. 2000 platform carried out a de novo analysis transcriptome data. The assembly generated 11,100 transcripts. These transcripts represent total 7876 unigenes...