Ionizing radiation is extensively employed in both diagnostic and therapeutic medical practices. The impact of this on collagen, a primary structural protein humans, remains underexplored, particularly at varying doses hydration states. This study explores the ionizing type I collagen fibrils three (diagnostic, therapeutic, sterilization) under two conditions using an engineered acellular membrane to reflect biological conditions. Techniques including atomic force microscopy (AFM),...

Abstract Stereotactic body radiotherapy (SBRT) is an emerging cancer treatment due to its logistical and potential therapeutic benefits as compared conventional radiotherapy. However, mechanism of action yet be fully understood, likely involving the ablation tumour microvasculature by higher doses per fraction used in SBRT. In this study, we hypothesized that longitudinal imaging quantification vascular architecture may elucidate relationship between response kinetics. Pancreatic human...

Preclinical intravital imaging such as microscopy and optical coherence tomography have proven to be valuable tools in cancer research for visualizing the tumor microenvironment its response therapy. These modalities micron-scale resolution but limited use clinic due their shallow penetration depth into tissue. More clinically applicable CT, MRI, PET much greater comparatively lower spatial (mm scale). To translate preclinical findings clinic, new methods must developed bridge this...

Abstract The dominant consequence of irradiating biological systems is cellular damage, yet microvascular damage begins to assume an increasingly important role as the radiation dose levels increase. This currently becoming more relevant in medicine with its pivot towards higher-dose-per-fraction/fewer fractions treatment paradigm (e.g., stereotactic body radiotherapy (SBRT)). We have thus developed a 3D preclinical imaging platform based on speckle-variance optical coherence tomography...

Preclinical intravital imaging such as microscopy and optical coherence tomography have proven to be valuable tools in cancer research for visualizing the tumor microenvironment its response therapy. These modalities micron-scale resolution but limited use clinic due their shallow penetration depth into tissue. More clinically applicable CT, MRI, PET much greater comparatively lower spatial (mm scale). To translate preclinical findings clinic, new methods must developed bridge this...

Dynamic contrast enhanced magnetic resonance imaging (DCE-MRI) is emerging as a valuable tool for non-invasive volumetric monitoring of the tumor vascular status and its therapeutic response. However, clinical utility DCE-MRI challenged by uncertainty in ability to quantify microvasculature ([Formula: see text] scale) given relatively poor spatial resolution (mm scale at best). To address this challenge, we directly compared parameter maps with co-registered micron-scale-resolution speckle...

Stereotactic body radiotherapy (SBRT) shows promise for increasing local tumour control many of the most lethal cancer types including pancreatic ductal carcinoma (PDA), compared to conventional radiotherapy. Yet SBRT radiation fractionation schedules may still be improved as its mechanism action remains largely unknown. It has been suggested that this accelerated hypofractionated treatment benefits from vascular damage (in particular blood capillaries ~10-30μm in diameter). We therefore...

SignificanceLymphatic and peripheral nervous system imaging is of prime importance for monitoring various important pathologic processes including cancer development metastasis, response to therapy.AimOptical coherence tomography (OCT) a promising approach this task but challenged by the near-transparent nature these structures. Our aim detect differentiate semi-transparent materials using OCT texture analysis, toward label-free neurography lymphography.ApproachWe have recently demonstrated...

1 Abstract Window chamber models enable a range of preclinical in-vivo optical studies with high spatial resolution and contrast, most notably probing the tumour microenvironment (TME). However, there are multiple sources experimental variability that can affect quality resultant data, especially in context longitudinal data acquisition, where accurate registration between images acquired at different times is crucial to understanding changes spatially heterogeneous TME. Further, it...

Abstract Stereotactic body radiotherapy (SBRT) is an emerging cancer treatment due to its logistical and potential therapeutic benefits as compared conventional radiotherapy. However, mechanism of action yet be fully understood, likely involving the ablation tumour microvasculature by higher doses per fraction used in SBRT. In this study, we hypothesized that longitudinal imaging quantification vascular architecture may elucidate relationship between response kinetics. Pancreatic human...

Abstract Significance Lymphatic and peripheral nervous system imaging is of prime importance for monitoring various important pathologic processes including cancer development, metastasis, response to therapy. Aim Optical coherence tomography (OCT) a promising approach this task but challenged by the near-transparent nature these structures. Our aim detect differentiate semi-transparent materials using OCT texture analysis, towards label-free neurography lymphography. Approach We have...

OCT can monitor the tumor vascular response to stereotactic body radiotherapy (SBRT). We find correlations between microvascular metrics and MRI angiography macrovascular metrics, supporting use of in SBRT treatment monitoring.

Stereotactic body radiotherapy's radiobiological mechanism of action is unknown, impeding development adaptive irradiation schedules. A new vascular biomarker with predictive potential tumour radiation response and hypoxia extracted via OCT angiography longitudinally.