A5081459677- Enzyme Structure and Function
- Enzyme Production and Characterization
- Biochemical and Molecular Research
- Biopolymer Synthesis and Applications
- Amino Acid Enzymes and Metabolism
- Erythrocyte Function and Pathophysiology
- Carbohydrate Chemistry and Synthesis
- Glycosylation and Glycoproteins Research
- Spectroscopy Techniques in Biomedical and Chemical Research
- X-ray Diffraction in Crystallography
- Bee Products Chemical Analysis
- Fungal Biology and Applications
- Crystal structures of chemical compounds
- Research on Leishmaniasis Studies
- Tannin, Tannase and Anticancer Activities
- Curcumin's Biomedical Applications
- Silk-based biomaterials and applications
- Insect Pest Control Strategies
- Computational Drug Discovery Methods
- Polysaccharides and Plant Cell Walls
- Invertebrate Immune Response Mechanisms
- Biochemical effects in animals
- Trypanosoma species research and implications
- Enzyme function and inhibition
- Porphyrin Metabolism and Disorders
Synchrotron Light Research Institute
2009-2025
Suranaree University of Technology
2009
University of Edinburgh
2009
Institute of Structural and Molecular Biology
2009
Abstract Glycoside hydrolase family 1 (GH1) β-glucosidases play roles in many processes plants, such as chemical defense, alkaloid metabolism, hydrolysis of cell wall-derived oligosaccharides, phytohormone regulation, and lignification. However, the functions most 34 GH1 gene products rice (Oryza sativa) are unknown. Os3BGlu6, a β-glucosidase representing previously uncharacterized phylogenetic cluster GH1, was produced recombinant Escherichia coli. Os3BGlu6 hydrolyzed p-nitrophenyl...
Abstract Sericin, a silk protein from Bombyx mori (silkworms), has many applications, including cosmetics, anti-inflammation, and anti-cancer. Sericin complexes with nanoparticles have shown promise for breast cancer cell lines. Apoptosis, programmed death mechanism, stops growth. This study found that urea extract significantly affected HCT116 viability (IC 50 = 42.00 ± 0.002 µg/mL) caused apoptosis in over 80% of treated cells. S-FTIR analysis showed significant changes Sericin-treated...
Abstract ATP‐analogue inhibitors, Gefitinib (Iressa) and Erlotinib (Tarceva) had been approved for advanced metastatic nonsmall cell lung cancer (NSCLC) cells against tyrosine kinase domain of epidermal growth factor receptor (EGFR). Many techniques have developed to better understand the drug mechanism which is multistep, time‐consuming expensive. Herein, we performed Fourier‐transform infrared (FTIR) microscopy evaluating biochemical change on NSCLC (A549) after treatment. At levels that...
Abstract Rice BGlu1 β‐glucosidase nucleophile mutant E386G is a glycosynthase that can synthesize p ‐nitrophenyl ( NP)‐cellooligosaccharides of up to 11 residues. The X‐ray crystal structures the with and without α‐glucosyl fluoride were solved complex was found contain an ordered water molecule near position native structure, which likely stabilize departing fluoride. in complexes cellotetraose cellopentaose confirmed side chains N245, S334, Y341 interact glucosyl residues...
Bua Bok or Centella asiatica (CA) is an Asian vegetable with anti-inflammatory benefits. Asiaticoside, asiatic acid, madecassoside and madecassic have been characterised as major active ingredients a wide range of pharmacological advantages. In manufacturing processes, high-performance liquid chromatography (HPLC) chromatography-mass spectrometry (LCMS) are used to routinely determine the compounds in raw materials.This research aims explore properties, characterise metabolites observe...
Janus kinase (JAK) deregulation of the JAK/signal transducers and activators transcription pathway leads to myelofibrosis that can be treated by JAK inhibitors including Ruxolitinib Tofacitinib. Even though both are effective against myelofibrosis, each them has a different mode action in cells. is an inhibitor for selective JAK1/2, Tofacitinib JAK3. This study evaluated chemical fingerprints TF-1 cells after treatments synchrotron Fourier transform infrared microspectroscopy (S-FTIR)...
Mycelia were cultivated from a Thai wild mushroom identified as Ganoderma australe based on polymerase chain reaction (PCR) and morphological analyses. The mycelial extracts examined for their active ingredients using liquid chromatography-tandem mass spectrometry (LC‒MS/MS) method. This revealed the presence of lovastatin tentative compounds including p-coumaric, nicotinamide, gamma-aminobutyric acid, choline, nucleosides, amino acids, saccharides. had an inhibitory effect activity HMG-CoA...
Sulfated galactans (SG) isolated from red alga Gracilaria fisheri have been reported to inhibit the growth of cholangiocarcinoma (CCA) cells, which was similar epidermal factor receptor (EGFR)-targeted drug, cetuximab. Herein, we studied anti-cancer potency SG compared Biological studies demonstrated and cetuximab had inhibition mechanisms in CCA cells by down-regulating EGFR/ERK pathway, combined treatment induced a greater effect. The molecular docking study revealed that binds...
Fragrant rice (Oryza sativa L.) betaine aldehyde dehydrogenase 2 (OsBADH2) is a key enzyme in the synthesis of fragrance aroma compounds. The extremely low activity OsBADH2 catalyzing oxidation acetaldehyde believed to be crucial for accumulation volatile compound 2-acetyl-1-pyrroline (2AP) many scented plants, including fragrant rice. Recombinant was expressed Escherichia coli as an N-terminal hexahistidine fusion protein, purified using Ni Sepharose affinity chromatography and crystallized...
Abstract With the increased usage of silver nanoparticles (AgNPs), potential impacts released AgNPs in environment are increasingly concerned, especially to natural living organisms. Since properties significantly depend on their sizes, this work aimed compare effects 5-nm and 70‐nm toxicity bioaccumulation Hoplobatrachus rugulosus , edible East Asian Bullfrog. The synthesized were characterized by X‐ray diffraction selected area electron analyses. Both 5‐nm caused mortality, reduced growth,...
Marine green turtle (Chelonia mydas) egg-white ribonuclease (GTRNase) was crystallized from 1.1 M ammonium sulfate pH 5.5 and 30% glycerol using the sitting-drop vapour-diffusion method. The structure of GTRNase has been solved at 1.60 A resolution by molecular-replacement technique a model based on RNase 5 (murine angiogenin) Mus musculus (46% identity). crystal belonged to monoclinic space group C2, with unit-cell parameters = 86.271, b 34.174, c 39.738 A, alpha 90, beta 102, gamma 90...