A5060235077- Cardiac Valve Diseases and Treatments
- Nitric Oxide and Endothelin Effects
- Cardiac and Coronary Surgery Techniques
- Electrospun Nanofibers in Biomedical Applications
- Cardiac Ischemia and Reperfusion
- Pulmonary Hypertension Research and Treatments
- Aortic Disease and Treatment Approaches
- Tissue Engineering and Regenerative Medicine
- Renin-Angiotensin System Studies
- Cardiovascular Function and Risk Factors
- Receptor Mechanisms and Signaling
- Coronary Interventions and Diagnostics
- Cardiac Fibrosis and Remodeling
- Transplantation: Methods and Outcomes
- Congenital heart defects research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Congenital Heart Disease Studies
- Infective Endocarditis Diagnosis and Management
- Orthopaedic implants and arthroplasty
- Neuropeptides and Animal Physiology
- Atrial Fibrillation Management and Outcomes
- Cardiac, Anesthesia and Surgical Outcomes
- Cardiovascular Issues in Pregnancy
- Connective tissue disorders research
- Mesenchymal stem cell research
Imperial College London
2014-2025
Harefield Hospital
2012-2022
Qatar Foundation
2013-2015
University College London
1989-2015
King's College London
2015
Maastricht University
2015
British Heart Foundation
2015
Great Ormond Street Hospital
2015
National Institutes of Health
1994-2014
Qatar Cardiovascular Research Center
2012-2014
Rationale: Matrix vesicles (MVs), secreted by vascular smooth muscle cells (VSMCs), form the first nidus for mineralization and fetuin-A, a potent circulating inhibitor of calcification, is specifically loaded into MVs. However, processes fetuin-A intracellular trafficking MV biogenesis are poorly understood. Objective: The objective this study to investigate regulation, role, in VSMC calcification. Methods Results: Alexa488-labeled was internalized human VSMCs, trafficked via endosomal...
Background— Calcific aortic valve stenosis is a common disease in the elderly and characterized by progressive calcification fibrous thickening of valve, but cellular molecular mechanisms are not fully understood. We hypothesized that human interstitial cells (ICs) able to differentiate into osteoblast-like through influence defined mediators this process can be modulated pharmacologically. Methods Results— To test hypothesis, we treated primary cultures ICs with osteogenic media, bone...
Arterial endothelial cells maintain vascular homeostasis and vessel tone in part through the secretion of nitric oxide (NO). In this study, we determined how aortic valve (VEC) regulate interstitial cell (VIC) phenotype matrix calcification NO. Using an anchored vitro collagen hydrogel culture system, demonstrate that three-dimensionally cultured porcine VIC do not calcify osteogenic medium unless under mechanical stress. Co-culture with VEC, however, significantly attenuated inhibition...
Crosstalk between cardiomyocytes and fibroblasts in physiological conditions during disease remains poorly defined. Previous studies have shown that myocytes interact via paracrine communication, but several experimental confounding factors, including the use of immature induction alpha-smooth muscle actin (α-SMA) expression by prolonged culture, hindered our understanding this phenomenon. We hypothesize myofibroblasts differentially affect viability, volume, Ca2+ handling soluble mediators....
Abstract Polydimethylsiloxane (PDMS) is widely used in biomedical science and can form composites that have broad applicability. One promising application where PDMS offer several advantages optical ultrasound generation via the photoacoustic effect. Here, methods to create these are reviewed classified. It highlighted how be applied a range of substrates, from micrometer‐scale, temperature‐sensitive fibers centimeter‐scale curved planar surfaces. The resulting enabled all‐optical imaging...
Similar risk factors and mediators are involved in calcific aortic stenosis (CAS) atherosclerosis. Since normal valves harbour a low percentage of smooth muscle cells (SMCs), we hypothesize that the SMC phenotype participates pathogenesis CAS.We analysed 12 22 calcified for markers expression co-activators gene expression, myocardin myocardin-related transcription (MRTF-A/B). Transforming growth factor β (TGFβ1) was used to upregulate valve interstitial (VICs) transmission electron...
Cardiac expression of angiotensin II (Ang II) AT1 and AT2 receptor subtypes is species dependent, changes in their relative proportion may influence myocardial hypertrophy fibrosis. Regional differences the distribution Ang receptors normal failing human heart were assessed using 125I-(Sar1,Ile8)Ang binding quantitative autoradiography. Receptor distinguished by affinity for selective nonpeptide antagonists (losartan PD123319) sensitivity to dithiothreitol. Ventricular atrial tissues...
The cells that reside within valve cusps play an integral role in the durability and function of heart valves. There are principally two types found cusp tissue: endothelial cover surface interstitial (ICs) form a network extracellular matrix (ECM) body cusp. Both cell exhibit unique functions unlike those other ICs throughout body. express complex pattern cell-surface, cytoskeletal muscle proteins. They able to bind to, communicate with, each ECM. on outflow inflow surfaces differ from one...
Journal Article 5-Hydroxytryptamine receptor profile in healthy and diseased human epicardial coronary arteries Get access Adrian H Chester, Chester Correspondence to: Professor Yacoub Search for other works by this author on: Oxford Academic PubMed Google Scholar Graeme R Martin, Martin Mikeal Bodelsson, Bodelsson Brigitta Arneklo-Nobin, Arneklo-Nobin Samad Tadjkarimi, Tadjkarimi Kenneth Tornebrandt, Tornebrandt Magdi Cardiovascular Research, Volume 24, Issue 11, November 1990, Pages...
Raman spectroscopy of tissue biochemistry reveals the interplay between atherosclerosis and medial calcification in human aorta.
Valve interstitial cells (VICs) are fibroblastic in nature however culture it is widely accepted that they differentiate into a myofibroblastic phenotype. This study assessed fibroblast media formulation for its ability to maintain the phenotype and function of VICs as intact healthy valve. Normal human were cultured separately standard DMEM consisting FGF2 (10ng/ml), insulin (50ng/ml) 2% FCS at least week. Cell morphology, aspect ratio, size, levels distribution protein expression,...
Background Oestrogen-replacement therapy is associated with a reduced incidence of cardiovascular disease. The acute administration oestrogen improves myocardial ischemia in women coronary heart In this study we investigated the relaxing effect oestradiol-17β on human arteries vitro and determined role endothelial modulation relaxation by using isolated arteries. Methods Atherosclerosis-free epicardial from men were removed patients undergoing or combined lung transplantation. cut into ring...