A5009432106- Bone Tissue Engineering Materials
- Parathyroid Disorders and Treatments
- Bone health and osteoporosis research
- Calcium Carbonate Crystallization and Inhibition
- Cardiac Valve Diseases and Treatments
- Orthopaedic implants and arthroplasty
- Cellular Mechanics and Interactions
- Bone and Dental Protein Studies
- Nanoplatforms for cancer theranostics
- Electrospun Nanofibers in Biomedical Applications
- Medical Imaging and Pathology Studies
- Extracellular vesicles in disease
- Cell Adhesion Molecules Research
- Aortic Disease and Treatment Approaches
- Advanced X-ray and CT Imaging
- Photoacoustic and Ultrasonic Imaging
- Paleontology and Evolutionary Biology
- Dermatological and Skeletal Disorders
- Caveolin-1 and cellular processes
- Silk-based biomaterials and applications
- 3D Printing in Biomedical Research
- Bone health and treatments
- Turtle Biology and Conservation
- Luminescence Properties of Advanced Materials
- Advanced Electron Microscopy Techniques and Applications
University College London
2016-2025
London Centre for Nanotechnology
2022-2025
Transnational Press London
2016-2019
Imperial College London
2010-2018
NIHR Imperial Biomedical Research Centre
2014-2018
Eindhoven University of Technology
2016
Harvard University
2016
University of Toronto
2016
King's College London
2015
Maastricht University
2015
Rationale: Matrix vesicles (MVs), secreted by vascular smooth muscle cells (VSMCs), form the first nidus for mineralization and fetuin-A, a potent circulating inhibitor of calcification, is specifically loaded into MVs. However, processes fetuin-A intracellular trafficking MV biogenesis are poorly understood. Objective: The objective this study to investigate regulation, role, in VSMC calcification. Methods Results: Alexa488-labeled was internalized human VSMCs, trafficked via endosomal...
Self-assembled biomaterials are an important class of materials that can be injected and formed in situ. However, they often not able to meet the mechanical properties necessary for many biological applications, losing at low strains. We synthesized hybrid hydrogels consisting a poly(γ-glutamic acid) polymer network physically cross-linked via grafted self-assembling β-sheet peptides provide non-covalent cross-linking through assembly, reinforced with backbone improve strain stability. By...
Hydroxyapatite nodules in retinal drusen may indicate risk for progression to advanced age-related macular degeneration.
Gold quantum dots exhibit distinctive optical and magnetic behaviors compared with larger gold nanoparticles. However, their unfavorable interaction living systems lack of stability in aqueous solvents has so far prevented adoption biology medicine. Here, a simple synthetic pathway integrates within mesoporous silica shell, alongside nanoparticles the shell's central cavity. This "quantum rattle" structure is stable solutions, does not elicit cell toxicity, preserves attractive near-infrared...
Abstract Polydimethylsiloxane (PDMS) is widely used in biomedical science and can form composites that have broad applicability. One promising application where PDMS offer several advantages optical ultrasound generation via the photoacoustic effect. Here, methods to create these are reviewed classified. It highlighted how be applied a range of substrates, from micrometer‐scale, temperature‐sensitive fibers centimeter‐scale curved planar surfaces. The resulting enabled all‐optical imaging...
Abstract Extracellular vesicles (EVs) have recently gained significant attention as important mediators of intercellular communication, potential drug carriers, and disease biomarkers. These natural cell‐derived nanoparticles are postulated to be biocompatible, stable under physiological conditions, show reduced immunogenicity compared other synthetic nanoparticles. Although initial clinical trials ongoing, the use EVs for therapeutic applications may limited due undesired off‐target...
Protein-mediated tethering of vesicles in the extracellular matrix contributes to microcalcification formation.
Abstract Exceptionally preserved organic remains are known throughout the vertebrate fossil record, and recently, evidence has emerged that such soft tissue might contain original components. We examined samples from eight Cretaceous dinosaur bones using nano-analytical techniques; not exceptionally show no external indication of tissue. In one sample, we observe structures consistent with endogenous collagen fibre displaying ∼67 nm banding, indicating possible preservation quaternary...
Raman spectroscopy of tissue biochemistry reveals the interplay between atherosclerosis and medial calcification in human aorta.
Significance Laminin-111 is one of the first extracellular matrix proteins expressed during embryogenesis and has been studied for decades, mainly because its major role in assembling basement membrane, but also it now become most popular cell culture substrates embryonic stem cells. However, considering importance this protein, laminin-111 plays remodeling—which not only great interest when seeking to understand cell–matrix interactions, using laminin as a substrate tissue engineering—is...
Collagen accumulation and calcification are major determinants of atherosclerotic plaque stability. Extracellular vesicle (EV)-derived microcalcifications in the collagen-poor fibrous cap may promote rupture. In this study, we hypothesize that collagen receptor discoidin domain receptor-1 (DDR-1) regulates deposition release calcifying EVs by vascular smooth muscle cells (SMCs) through transforming growth factor-β (TGF-β) pathway.SMCs from carotid arteries DDR-1(-/-) mice wild-type...
In this work, we studied the surface charge of α-alumina treated with carboxylic acids different carbon chain length. The results show possibility controlling charges alumina by using concentrations or changing size acids. We also report that part acid found on is strongly bound, therefore making it possible to obtain pH-resistant samples an isoelectric point (IEP) 5.5. It found, IEP values obtained for modified have a linear correlation number atoms dicarboxylic up five atoms. From...
Neuronatin (Nnat) is an imprinted gene implicated in human obesity and widely expressed neuroendocrine metabolic tissues a hormone- nutrient-sensitive manner. However, its molecular cellular functions precise role organismal physiology remain only partly defined. Here we demonstrate that mice lacking Nnat globally or specifically β cells display impaired glucose-stimulated insulin secretion leading to defective glucose handling under conditions of nutrient excess. In contrast, report no...
Despite decades of research, wound complications remain a major cause postoperative mortality, especially in the face multiple comorbidities. Addressing issue anastomotic leakages and impaired healing from new angle is great interest with prospect having direct impact on patient outcome. Recently, aqueous suspensions silica iron oxide nanoparticles have been employed to connect biological tissue by serving as an adhesive layer eventually leading macroscopic gluing tissue. In this work, we...
This work reports the synthesis of lithium-silicate glass, containing 10 mol% Li 2 O by sol-gel process, intended for regeneration cartilage. Lithium citrate and lithium nitrate were selected as precursors. The effects precursor on resulting glass structure, morphology, dissolution behaviour, chondrocyte viability proliferation, investigated. When was used, mesoporous a network modifier obtained, whereas use produced relatively dense glass-ceramic with presence metasilicate, shown X-ray...
Abstract Cancer cell extravasation, a key step in the metastatic cascade, involves cancer arrest on endothelium, transendothelial migration (TEM), followed by invasion into subendothelial extracellular matrix (ECM) of distant tissues. While research has mostly focused biomechanical interactions between tumor cells (TCs) and ECM, particularly at primary site, very little is known about mechanical properties endothelial ECM how they contribute to extravasation process. Here, an integrated...
Embryonic stem cells (ESCs) can differentiate into all cell types of the body and, therefore, hold tremendous promise for cell-based regenerative medicine therapies. One significant challenge that should be addressed before using ESCs in clinic is to improve methods efficiently and effectively directing differentiation this heterogeneous population. The work presented here examines potential harnessing naturally derived extracellular vesicles deliver genetic material from mature...