A5024337014- Telomeres, Telomerase, and Senescence
- Chromosomal and Genetic Variations
- DNA Repair Mechanisms
- Advanced biosensing and bioanalysis techniques
- Urban Green Space and Health
- Genomics and Phylogenetic Studies
- Urban Heat Island Mitigation
- Mitochondrial Function and Pathology
- Kruppel-like factors research
- RNA regulation and disease
- Viral Infectious Diseases and Gene Expression in Insects
- Genomic variations and chromosomal abnormalities
- Animal Genetics and Reproduction
- Noise Effects and Management
- Analytical Methods in Pharmaceuticals
- Click Chemistry and Applications
- Peptidase Inhibition and Analysis
- Computational Drug Discovery Methods
- NF-κB Signaling Pathways
- CRISPR and Genetic Engineering
- Carcinogens and Genotoxicity Assessment
- Cell Adhesion Molecules Research
- Neurological Disease Mechanisms and Treatments
- DNA and Nucleic Acid Chemistry
- Cancer-related Molecular Pathways
IFOM
2020-2023
University of Genoa
2023
University of Pavia
2015
Istituto di Genetica Molecolare
2015
Abstract Extrachromosomal telomeric circles are commonly invoked as important players in telomere maintenance, but their origin has remained elusive. Using electron microscopy analysis on purified telomeres we show that, apart from known structures, repeats accumulate internal loops (i-loops) that occur the proximity of nicks and single-stranded DNA gaps. I-loops induced by damage at normal represent majority structures detected ALT (Alternative Lengthening Telomeres) tumor cells. Our data...
The telomerase reverse transcriptase elongates telomeres to prevent replicative senescence. This process requires exposure of the 3'-end, which is thought occur when two sister are generated at replication completion. Using two-dimensional agarose gel electrophoresis (2D-gels) and electron microscopy, we found that telomeric repeats hotspots for fork reversal. Fork reversal generates 3' ends before To verify whether these elongated by telomerase, probed de novo synthesis in situ...
Sustainability brings together three dimensions: environment, economy and society, as according to the Agenda 21, a comprehensive plan of action be taken globally, nationally, locally by organizations United Nations System, governments, major groups in every area which humans impact environment. To define whether an intervention or process is ‘sustainable’, these spheres must co-exist equally contemplated. Green roofs have been extensively studied from environmental economic perspectives,...
Moderate DNA damage resulting from metabolic activities or sub-lethal doses of exogenous insults may eventually lead to cancer onset. Human 46BR.1G1 cells bear a mutation in replicative ligase I (LigI) which results low levels replication-dependent damage. This replication stress elicits constitutive phosphorylation the ataxia telangiectasia mutated (ATM) checkpoint kinase that fails arrest cell cycle progression activate apoptosis senescence. Stable transfection wild type LigI, as 7A3...
Centromeres are key elements for chromosome segregation. Canonical centromeres built over long-stretches of tandem repetitive arrays. Despite being quite abundant compared to other loci, centromere sequences overall still represent only 2 5% the human genome, therefore studying their genetic and epigenetic features is a major challenge. Furthermore, sequencing centromeric regions requires high coverage fully analyze length sequence variations, this can be extremely costly. To bypass these...
Building Integrated Greenery systems (BIGs), such as green roofs and walls, present valuable solutions to the environmental challenges that we are facing today. These provide numerous benefits enhancing biodiversity, controlling Urban Heat Island phenomenon, improving air quality water management, decreasing energy consumption by roof insulation. Recently, academic researchers have predominantly centred on sustainability economic issues, often neglecting other socio-cultural aspects....
Abstract Centromeres are key elements for chromosome segregation. Canonical centromeres built over long-stretches of tandem repetitive arrays. Despite being quite abundant compared to other loci, centromere sequences overall still represent only 2 5% the human genome, therefore studying their genetic and epigenetic features is a major challenge. Furthermore, sequencing centromeric regions requires high coverage fully analyze length sequence variations, which can be extremely costly. To...
Abstract Extrachromosomal telomeric circles are commonly invoked as important players in telomere maintenance, but their origin has remained elusive. Using electron microscopy analysis on purified telomeres we show that, apart from known structures, repeats accumulate internal loops (i-loops), that occur proximity of nicks and single-stranded DNA gaps. I-loops induced by damage at normal represent the majority structures detected ALT (Alternative Lengthening Telomeres) tumor cells. Our data...