A5078644608- Insect and Pesticide Research
- Microtubule and mitosis dynamics
- Insect and Arachnid Ecology and Behavior
- Healthcare and Venom Research
- Genomics and Chromatin Dynamics
- Chromosomal and Genetic Variations
- Bee Products Chemical Analysis
- Mitochondrial Function and Pathology
- Plant and animal studies
- DNA Repair Mechanisms
- Neurobiology and Insect Physiology Research
- Photosynthetic Processes and Mechanisms
- Ubiquitin and proteasome pathways
- Plant-based Medicinal Research
- Nuclear Structure and Function
- Cancer, Hypoxia, and Metabolism
- Cellular transport and secretion
- Enzyme Production and Characterization
- Genetics and Neurodevelopmental Disorders
- Cancer Mechanisms and Therapy
- Growth Hormone and Insulin-like Growth Factors
- Cancer-related Molecular Pathways
- Phytase and its Applications
Max Planck Institute of Molecular Physiology
2016-2025
Max Planck Society
2022
Architecture et Fonction des Macromolécules Biologiques
2008-2009
Aix-Marseille Université
2009
Centre d’Immunologie de Marseille-Luminy
2008
Centre National de la Recherche Scientifique
2008
University of Hyogo
2008
Centre Hospitalier Régional et Universitaire de Nancy
2002
Centromeres are specialized chromosome loci that seed the kinetochore, a large protein complex effects segregation. A 16-subunit complex, constitutive centromere associated network (CCAN), connects between centromeric chromatin, marked by histone H3 variant CENP-A, and spindle-binding moiety of kinetochore. Here, we report cryo-electron microscopy structure human CCAN. We highlight unique features such as pseudo GTPase CENP-M how crucial CENP-C motif binds CENP-LN complex. The CCAN has...
Reflecting its pleiotropic functions, Polo-like kinase 1 (PLK1) localizes to various sub-cellular structures during mitosis. At kinetochores, PLK1 contributes microtubule attachments and mitotic checkpoint signaling. Previous studies identified a wealth of potential receptors at as well requirements for kinases, including BUB1, Aurora B, itself. Here, we combine ectopic localization, in vitro reconstitution, kinetochore localization demonstrate that most likely all the is recruited through...
The approximately thirty core subunits of kinetochores assemble on centromeric chromatin containing the histone H3 variant CENP-A and connect chromosomes with spindle microtubules. proximal 16-subunit CCAN (constitutive centromere associated network) creates a mechanically stable bridge between kinetochore's microtubule-binding machinery, 10-subunit KMN assembly. Here, we reconstituted stoichiometric 11-subunit human that forms when CENP-OPQUR complex binds to joint interface CENP-HIKM...
The centromere, a chromosome locus defined by the histone H3-like protein centromeric A (CENP-A), promotes assembly of kinetochore to bind microtubules during cell division. Centromere maintenance requires CENP-A be actively replenished dedicated machinery in early G
Abstract The centromere, a chromosome locus defined by the histone H3-like protein CENP-A, seeds kinetochore to bind microtubules during cell division. Centromere maintenance requires CENP-A be actively replenished dedicated machinery in early G1 cell-cycle phase, compensating for its two-fold dilution following DNA replication. Cyclin-dependent kinases (CDKs) limit deposition once per cycle and function as negative regulators outside G1. Antithetically, Polo-like kinase 1 (PLK1) promotes...
The FAcilitates Chromatin Transcription (FACT) complex is a dimeric histone chaperone that operates on chromatin during transcription and replication. FACT also interacts with specialized centromeric nucleosome containing the H3 variant centromere protein A (CENP-A) CENP-TW, two subunits of constitutive centromere–associated network (CCAN), 16-protein associated CENP-A. significance these interactions remains elusive. Here, we show has multiple additional binding sites CCAN. interaction CCAN...
The Polo-box domain (PBD) localizes Polo-like kinase 1 (PLK1) near mitotic substrates required for chromosome biorientation. Recent work on kinetochores showed PLK1 docking begins hierarchically at master motifs BUB1 and CENP-U. Whether have common molecular features remains poorly understood. Presence CENP-U of two neighbouring generated by initial CDK1 priming subsequent phosphorylation led us to hypothesize PBD dimerization might be involved. Using biochemical, biophysical, modelling...
Abstract Mitosis induces cellular rearrangements like spindle formation, Golgi fragmentation, and nuclear envelope breakdown. Similar to certain retroviruses, delivery during entry of human papillomavirus (HPV) genomes is facilitated by mitosis, which minor capsid protein L2 tethers viral DNA mitotic chromosomes. However, the mechanism genome tethering condensed chromosomes barely understood. It unclear, proteins facilitate this process or how regulated. This work identifies crucial...
During cell division, the microtubule cytoskeleton undergoes dramatic cycle-driven reorganizations of its architecture. Coordinated by changes in phosphorylation patterns a multitude associated proteins, mitotic spindle first self-assembles to capture chromosomes and then reorganizes anaphase as are segregated. A key protein for this reorganization is PRC1 which differentially phosphorylated kinases CDK1 PLK1. How state orchestrates not understood mechanistically. Here, we reconstitute vitro...
Centromeres are specialized chromosome loci that seed the kinetochore, a large protein complex effects segregation. The organization of interface between kinetochore and centromeric chromatin, marked by histone H3 variant CENP-A, remains incompletely understood. A 16-subunit complex, constitutive centromere associated network (CCAN), bridges CENP-A to spindle-binding moiety kinetochore. Here, we report cryo-electron microscopy structure human CCAN. We highlight unique features such as pseudo...
Abstract The FAcilitates Chromatin Transcription (FACT) complex is a dimeric histone chaperone that operates on chromatin during transcription and replication. FACT also interacts with specialized centromeric nucleosome containing the H3 variant CENP-A CENP-TW, two subunits of CCAN, 16-protein associated CENP-A. significance these interactions remains elusive. Here, we show has multiple additional binding sites CCAN. interaction CCAN strongly stimulated by casein kinase II (CK2)...
Poster Sessions 4 and 0.1 M TRIS-HCl buffer, pH 8.5.Diffraction data was collected to 2.4 Å resolution at beamline BL6A of Photon Factory.ERK1 structure solved by molecular replacement method using the ERK1 homology model as a probe.The structural refinement modification are currently in progress.A detailed comparison with that ERK2 will be reported.