Hot-pressed peanut meal protein hydrolysates are rich in Arg residue, but there is a lack of research on their α-glucosidase inhibitory activity. In this study, different proteases were used to produce hot-pressed (PMHs) evaluate the All PMHs showed good activity with best inhibition effect coming from dual enzyme system Alcalase and Neutrase an IC50 5.63 ± 0.19 mg/mL. The fractions highest separated purified using ultrafiltration cation exchange chromatography. Four novel peptides...

Soybean oil bodies (OBs), naturally pre-emulsified soybean oil, have been examined by many researchers owing to their great potential utilizations in food, cosmetics, pharmaceutical, and other applications requiring stable oil-in-water emulsions. This study was the first time confirm that lectin, Gly m Bd 28K (Bd 28K, one allergenic protein), Kunitz trypsin inhibitor (KTI), Bowman-Birk (BBI) were not contained extracted OBs even neutral pH aqueous extraction. It clarified well-known 30K...

After oil bodies (OBs) were extracted from ungerminated soybean by pH 6.8 extraction, it was found that 24 and 18 kDa oleosins hydrolyzed in the OBs, which contained many OB extrinsic proteins (i.e., lipoxygenase, β-conglycinin, γ-conglycinin, β-amylase, glycinin, Gly m Bd 30K (Bd 30K), P34 probable thiol protease (P34)) as well intrinsic proteins. In this study, some properties (specificity, optimal temperature) of proteases oleosin hydrolysis germination examined, high relationship between...

In this study, the macronutrients and micronutrients of pH 6.8, 8.0, 9.5, 11.0 extracted soybean oil bodies (OBs) were examined, revealing that OBs might be used as a natural carrier for bioactive components (unsaturated fatty acids, phospholipid, tocopherol, phytosterol). 6.8 (dry basis) contained 85.88% neutral lipid, 8.18% protein, 5.85% polar lipid (mainly phospholipid) by gravimetric analysis. The percentage was increased, while those protein decreased with increasing pH. Tocopherol...

Abstract During thermal treatment of soymilk, a rapid incorporation Kunitz trypsin inhibitor (KTI) into protein aggregates by covalent (disulfide bond, SS) and/or noncovalent interactions with other proteins is responsible for its fast inactivation activity (TIA). In contrast, the slow cleavage single Bowman–Birk (BBI) peptide bond TIA and chymotrypsin (CIA). this study, effects Ohmic heating (220 V, 50 Hz) on soymilk CIA were examined compared to induction cooker electric stove similar...

In this study, we successfully prepared high internal phase emulsions (HIPEs) with 78%–90% oil based on pumpkin seed bodies (PSOBs) extracted by neutral extraction at 2800×g to 31000×g. There were no effects the protein compositions and contents of PSOBs different centrifugal forces. included oleosin (14.5 kDa) extrinsic proteins (22 kDa, 27 33 45 kDa). The HIPE-PSOB 78% had relatively spherical droplets, which was similar PSOB 72% 1000×g, while 83%–90% displayed polygonal droplets. All...

Surface functionalization of low-density polyethylene (LDPE), silanized glass, and free-standing polyaniline (PANI) films via UV-induced graft copolymerization with 1,1'-bis(4-vinyl-benzyl)-4,4'-bipyridinium dichloride (VBV) was carried out. The vinyl group containing viologen synthesized by the double Anderson reaction using 4,4'-bipyridine benzyl chloride characterized elemental analysis, FTIR spectrum, X-ray photoelectron spectroscopy. VBV out placing plasma-pretreated substrate in...

Abstract Soybean oil body (SOB), rich in polyunsaturated fatty acids and biologically active substances, is used as a natural emulsifier food processing. In addition, SOB healthier than synthetic emulsifiers. However, the physical chemical properties of emulsion directly affect its application order to study effect water bath extraction (WBAE) on SOBs, effects WBAE method composition zeta potential, average particle size, oxidation stability, viscosity characteristics emulsions were...

The reaction of 50% oxidized polyaniline (emeraldine base, EB) with alkyl halide and viologen in the dry state was studied using X-ray photoelectron spectroscopy, UV−visible absorption sheet resistance measurements. To facilitate this study, a low-density polyethylene substrate first surface graft copolymerized vinylbenzyl chloride or moieties. A coating (PANI) then deposited on top film. Under UV irradiation heat treatment at 65 °C higher, PANI reacted and/or viologen, resulting its...

P34 probable thiol protease (P34) and Gly m Bd 30K (30K) show high relationship with the of 24 kDa oleosin soybean oil bodies. In this study, 9 day germinated was used to separate bioprocessed (P32) from (28K). Interestingly, P32 existed as dimer, whereas 28K monomer; a P32-rich sample had proteolytic activity cleavage site specificity (Lys-Thr oleosin), 28K-rich showed low activity; contained one protease. After mixing purified bodies, all dimers were dissociated bound oleosins form P32-24...

Peony seeds, an important oil resource, have been attracting much attention because of α-linolenic acid. Oil bodies (OBs), naturally pre-emulsified oils, great potential applications in the food industry. This study investigated effects extraction pH and Quillaja saponin (QS) on physicochemical properties peony body (POB) emulsions. POBs were extracted from raw milk at 4.0, 5.0, 6.0, 7.0 (named 4.0-, 5.0-, 6.0-, 7.0-POBs). All contained extrinsic proteins oleosins. The 4.0- 5.0-POB 23 kDa 38...

Soybean oil body (SOB) emulsions were prepared using OBs extracted at pH 11.0 and 7.0. The 11.0-SOB comprised oleosins, whereas 7.0-SOB extrinsic proteins oleosins. All SOB heated 60-100 °C for 15 min. Heating may lead to the release of from surface due heat-induced denaturation. total proportion α-helix β-sheets gradually decreased 77 (unheated) 36.2% (100 °C). During stomach digestion, protein hydrolysis was fast between 60 80 °C, it then slowed 90 100 °C; heating inhibited oleosin 7.0-...

Summary The use of two emulsifiers to fabricate a composite emulsifier is useful strategy used strengthen the emulsifying capacity. To investigate feasibility using soy protein isolate (SPI) stabilise Pickering emulsions stabilised by peptide nanoparticles (SPNs), SPI was introduced into SPN system SPI‐SPN complex emulsifiers, and effects on properties SPNs SPN‐stabilised were investigated with various oil fractions. addition significantly reduced particle sizes surface hydrophobicities....

The preparation of nanoscaled metal coatings and dispersions has been carried out via the photoinduced reduction salt solutions using viologen grafted on low density polyethylene (LDPE) films or dispersed in a poly(vinyl alcohol) (PVA) matrix. effects UV irradiation time concentration process were investigated for gold, platinum, palladium chloride solutions. LDPE after reaction with characterized X-ray photoelectron spectroscopy, scanning electron microscopy, atomic force energy-dispersive...