A5102885340- Genomics and Chromatin Dynamics
- Microtubule and mitosis dynamics
- DNA Repair Mechanisms
- RNA Research and Splicing
- Agriculture and Biological Studies
- Dermatologic Treatments and Research
- Pesticide Exposure and Toxicity
- RNA and protein synthesis mechanisms
- Glycosylation and Glycoproteins Research
- CRISPR and Genetic Engineering
- Cancer-related Molecular Pathways
- Cellular Mechanics and Interactions
- melanin and skin pigmentation
- Ubiquitin and proteasome pathways
- Carcinogens and Genotoxicity Assessment
- Chromosomal and Genetic Variations
- interferon and immune responses
- Plant Genetic and Mutation Studies
- Skin Protection and Aging
- Animal testing and alternatives
Japanese Foundation For Cancer Research
2013-2024
Ministry of Agriculture, Forestry and Fisheries
2019
Niigata University
2016
Suzuki (Japan)
2004-2013
The structural maintenance of chromosomes (SMC) proteins constitute the core critical complexes involved in organization chromosomes. In yeast, Smc5/6 complex is known to mediate repair DNA breaks and replication repetitive genomic regions, including ribosomal loci telomeres. mammalian cells, which have diverse genome structure scale from has also been implicated damage response, but its further function unchallenged conditions remains elusive. this study, we addressed behavior during cell...
The chromokinesin KIF4A has been implicated in shaping mitotic chromosomes, but its functional relationship to condensin complexes remains controversial. Here, we found that, mitosis, associates with I not II. Mutational analyses indicated that the enrichment of chromosomal axes depends on association a way likely involves motor activity. Remarkably, this interaction is required for confer physiological properties chromosomes. These observations provide an insight into how enriched at and...
Abstract Chromosomal instability (CIN) is an outstanding feature of cancer, which relates to intratumor heterogeneity, therapeutic resistance, and poor prognosis. While cancer cells can proliferate with CIN, experimentally induced aneuploidy known suppress cell growth, leading the idea that extreme ploidy changes are detrimental cells. One major etiologies CIN abnormal kinetochore microtubule attachments, followed by chromosome missegregation. To avoid this, have evolved a robust system...
The Food Safety Commission (FSC) was founded in 2003 to conduct the risk assessment of chemicals food and products also residues agricultural chemicals. Genotoxicity is one component overall process. Historically, genotoxicity has been limited mainly qualitative hazard identification. We are proposing a strategy for when chemical classified as genotoxic carcinogen acceptable daily intake (ADI) cannot be set because worldwide consensus not obtained on existence threshold DNA direct-acting...
Protein phosphorylation is the prime post-translational modification to drive cell division. Identification of phosphorylated proteins and their related kinases has uncovered molecular networks underlying mitotic processes, including chromosome assembly. Here we aimed identify phosphoproteins from a chromosome-enriched lysate biochemically using mass spectrometry. We employed Polo-box domain (PBD) Polo-like kinase tether in lysate. Resulting candidates included number chromosomal that would...
Blood flow stagnation is said to be an important factor for the incidence of dark eye circles, but there are few reports, which have studied circles and relationship blood flow. We examined dermatologically using a non‐invasive method made clear related it. also effect newly developed cosmetics on improvement circles. Melanin haemoglobin content, oxygen saturation rate were measured compare with normal control. An increased amount decreased observed at site that was slow in comparison cheek....
When and how sister chromatid resolution occurs after DNA replication is a fundamental question. Stanyte et al. (2018. J. Cell Biol. https://doi.org/10.1083/jcb.201801157) used CRISPR/Cas9 technology to label track genomic loci in live cells throughout the cell cycle, shedding light on linked mitotic organization.
Abstract Polo‐like kinase 1 (Plk1) is a mitotic that has multiple functions throughout the cell cycle. Catalytic activation of Plk1 known to be regulated by phosphorylation domain, including Thr210, and releasing domain from its inhibitory polo‐box domain. However, how activated fulfill proper roles, in time space, not well understood. In this study, we unintentionally found expression constitutively active form human toxic bacterial cells, such cells contained point mutations alleviate...
The spindle-assembly checkpoint facilitates mitotic fidelity by delaying anaphase onset in response to microtubule vacancy at kinetochores. Following attachment, kinetochores receive microtubule-derived forces, which causes undergo repetitive cycles of deformations, the motion referred as kinetochore stretching. nature forces and relevance relating this are not well understood. Here we show that stretching occurs within a framework single end-on attached kinetochores, irrespectively poleward...