A5062175578- Molecular Biology Techniques and Applications
- Biosensors and Analytical Detection
- Innovative Microfluidic and Catalytic Techniques Innovation
- bioluminescence and chemiluminescence research
- Advanced biosensing and bioanalysis techniques
- Microfluidic and Capillary Electrophoresis Applications
- Blood disorders and treatments
- Bacillus and Francisella bacterial research
- Oral and gingival health research
- Virus-based gene therapy research
- Identification and Quantification in Food
- Adipose Tissue and Metabolism
- Enzyme Production and Characterization
- Ion channel regulation and function
- Microfluidic and Bio-sensing Technologies
- Pancreatic function and diabetes
- Salmonella and Campylobacter epidemiology
- 3D Printing in Biomedical Research
- Cell Adhesion Molecules Research
- Aldose Reductase and Taurine
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Genomics and Phylogenetic Studies
- RNA and protein synthesis mechanisms
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Vitamin C and Antioxidants Research
Kumamoto Prefectural Police
2018-2025
Hiroshima University
1994-2024
Kyoto Institute of Technology
2007
We developed an ultrasensitive bioluminescence assay of ATP by employing (i) adenylate kinase (ADK) for converting AMP+ATP to two molecules ADP, (ii) polyphosphate (polyP) (PPK) ADP back (ATP amplification), and (iii) a commercially available firefly luciferase. A highly purified PPK-ADK fusion protein efficiently amplified ATP, resulting in high levels the luciferase reaction. The present method, which was approximately 10,000-fold more sensitive than conventional assay, allowed us detect...
We developed novel aptamer-based assays for semen identification and demonstrated that aptamers can be useful tools body fluid identification.
We examined the systematic assay of reporter gene for assessment heavy metals and organic chemical pollutants using plasmids carrying stress-responsive elements fused to green fluorescence protein (GFP) as follows: metallothionein (MTIIA), heme oxigenase-1 (HO-1), quinone reductase (ARE), c-fos genes. The treatment COS7 cells in which promoter-, ARE-, or HO-1 enhancer-fused GFP with a low concentration NaAsO2 was introduced led detection fluorescent cells, an agrichemical paraquat enhanced...
In forensic DNA testing, PCR-based multilocus short tandem repeat (STR) profiling kits are generally used to analyze autosomal and Y-chromosomal profiles. Because these offer high sensitivity discriminatory power, laboratories require strict quality control for testing. Surfaces of laboratory work areas equipment occasionally contaminated with PCR products during The transfer residual into electrophoresis samples or subsequent PCRs causes errors in the typing interpretation Therefore, should...