A5046394307- Alcohol Consumption and Health Effects
- Forensic Toxicology and Drug Analysis
- HIV Research and Treatment
- Pancreatic and Hepatic Oncology Research
- Liver Disease Diagnosis and Treatment
- Metabolomics and Mass Spectrometry Studies
- Protease and Inhibitor Mechanisms
- Endoplasmic Reticulum Stress and Disease
- Psychedelics and Drug Studies
- Monoclonal and Polyclonal Antibodies Research
- Pancreatitis Pathology and Treatment
- Trypanosoma species research and implications
- vaccines and immunoinformatics approaches
- Mass Spectrometry Techniques and Applications
- Research on Leishmaniasis Studies
- PI3K/AKT/mTOR signaling in cancer
- Plant-based Medicinal Research
- Pain Management and Opioid Use
- Neuroendocrine Tumor Research Advances
- Glycosylation and Glycoproteins Research
- Protein Kinase Regulation and GTPase Signaling
- Cannabis and Cannabinoid Research
- Cassava research and cyanide
- Melamine detection and toxicity
- Analytical Chemistry and Chromatography
University Hospital Heidelberg
2020-2024
Heidelberg University
2020-2024
Poorly immunogenic small molecules pose challenges for the production of clinically efficacious vaccines and antibodies. To address this, we generate an immunization platform derived from surface coat African trypanosome. Through sortase-based conjugation target to variant glycoprotein (VSG) trypanosome coat, develop VSG-immunogen array by sortase tagging (VAST). VAST elicits antigen-specific memory B cells antibodies in a murine model after deploying poorly molecule fentanyl as proof...
Ethyl glucuronide (EtG), ethyl sulfate (EtS) and phosphatidylethanol (PEth) are considered specific direct biomarkers for detecting alcohol consumption. However, PEth, which is produced in red blood cells (RBC), varies considerably between patients unknown reasons. We here studied various confounders of PEth elimination including fibrosis after withdrawal.EtG, EtS together with routine laboratory clinical parameters were 100 Caucasian heavy drinkers prior detoxification. In addition, stage...
5-(2-Aminopropyl)benzofuran (5-APB) and 6-(2-aminopropyl)benzofuran (6-APB) are benzofuran analogues of amphetamine belong to the category new psychoactive substances. Despite already published fatal 5- 6-APB intoxication after consumption both substances in most cases, no sensitive method for simultaneous detection quantification these compounds human blood samples has yet been developed. Therefore, an easy fast sample preparation specific high-performance liquid chromatography tandem mass...
Alpha-1 antitrypsin deficiency is an inherited disorder caused by alpha-1 (AAT) mutations. We analysed the association between alcohol intake and liver-related parameters in individuals with heterozygous/homozygous Pi*Z AAT variant (Pi*MZ/Pi*ZZ genotype) found United Kingdom Biobank European Alpha1 liver consortium.
As alcohol is the most common addictive substance worldwide, it inevitable to advance established research. New and more substantial analytical methods can be applied reply complex questions in legal or forensic contexts. Therefore, an method for simultaneous determination of four different biomarkers-ethyl glucuronide, ethyl sulfate, N-acetyltaurine, 16:0/18:1-phosphatidylethanol-in human blood was developed, validated, verified. Despite chemical properties analytes, a specific via...
The congener analysis is routinely used for the determination of volatile compounds in body fluids and beverages forensic investigations. Although intoxications with cyanide via smoke inhalation or ingestion salts are frequently encountered medicine, inclusion hydrogen this was never studied detail. In work, a very simple, fast, sensitive quantification method headspace gas chromatography flame ionization detection whole blood-was developed validated. contrast to standard sample preparation...
Pancreatic surgery is complicated by untreated fluid leakage, but no tenable techniques exist to detect and close leakage sites during surgery. A novel hydrogel called SmartPAN has been developed meet this need here assessed for safety before trials on human patients. First, resazurin assays were used test the cytotoxic effects of SmartPAN's active bromothymol blue (BTB) indicator its solution phosphate-buffered saline (PBS) normal (HPDE: pancreatic duct epithelial) or carcinomic (FAMPAC)...
Gamma-hydroxybutyric acid (GHB) is a common drug of abuse, and the detection consumption or administration longstanding research objective in clinical forensic toxicology. However, until now, short window GHB could not be enlarged by use metabolites. Therefore, new biomarkers for intake are needed. In analogy to phosphatidylethanols as long-time ethanol, phospholipids with might represent promising compound class. While availability reference compounds often represents bottleneck...
Einleitung/Ziele Da Alpha1-Antitrypsin-Mangel (AATM) das Risiko einer Lebererkrankung erhöht, haben wir untersucht, inwiefern Alkoholkonsum den Leberphänotyp bei Erwachsenen mit der heterozygoten/homozygoten Pi*Z-Variante (Pi*MZ/Pi*ZZ-Genotyp) in United Kingdom Biobank (UKB) und dem europäischen Alpha1-Leberkonsortium modifiziert.
Summary Poorly antigenic small molecules pose challenges for the production of clinically efficacious antibodies. To address this, we have developed an immunization platform derived from surface coat African trypanosome. Through sortase-based conjugation antigens to trypanosome protein variant glycoprotein (VSG), created VAST ( V SG-immunogen A rray by S ortase T agging). We used elicit protective immunity effects fentanyl. Immunizing mice with fentanyl-VAST generated serological memory and...
Poorly antigenic small molecules pose challenges for the production of clinically efficacious antibodies. To address this, we have developed an immunization platform derived from surface coat African trypanosome. Through sortase-based conjugation antigens to trypanosome protein variant glycoprotein (VSG), created VAST (VSG-immunogen Array by Sortase Tagging). As a proof concept, applied potent opioid fentanyl. Immunizing mice with fentanyl-VAST generated serological memory and protection...
Introduction Pancreatic resections are an important field of surgery worldwide to treat a variety benign and malignant diseases. Postoperative pancreatic fistula (POPF) remains frequent critical complication after partial pancreatectomy affects up 50% patients. POPF increases mortality, prolongs the postoperative hospital stay is associated with significant economic burden. Despite various scientific approaches clinical strategies, it has not yet been possible develop effective preventive...