A5070508469- Cellular transport and secretion
- Lipid Membrane Structure and Behavior
- Gold and Silver Nanoparticles Synthesis and Applications
- Neuroscience and Neuropharmacology Research
- Photoreceptor and optogenetics research
- Alzheimer's disease research and treatments
- Plasmonic and Surface Plasmon Research
- Tryptophan and brain disorders
- Advanced biosensing and bioanalysis techniques
- Photonic and Optical Devices
- Neural dynamics and brain function
- Law and Political Science
- Digitalization, Law, and Regulation
- Microtubule and mitosis dynamics
- Cell Image Analysis Techniques
- Quantum Dots Synthesis And Properties
- Medical and Health Sciences Research
- Stress Responses and Cortisol
- Calcium signaling and nucleotide metabolism
- Photoacoustic and Ultrasonic Imaging
- Photonic Crystals and Applications
- Cellular Mechanics and Interactions
- Nanoparticles: synthesis and applications
- Sphingolipid Metabolism and Signaling
- S100 Proteins and Annexins
University of Wuppertal
2019-2024
Center for Free-Electron Laser Science
2023
Universität Hamburg
2023
Johannes Gutenberg University Mainz
2009-2023
Rohde & Schwarz (Germany)
2015-2022
Friedrich-Alexander-Universität Erlangen-Nürnberg
2002-2021
Kuwait University
2010-2019
Jena University Hospital
2015-2018
University of Applied Sciences Mainz
2008-2016
Zentrum für Datenverarbeitung
2015
The ultimate detection limit in analytic chemistry and biology is the single molecule. Commonly, fluorescent dye labels or enzymatic amplification are employed. This requires additional labeling of analyte, which modifies species under investigation therefore influences biological processes. Here, we utilize gold nanoparticles to detect unlabeled proteins with extremely high temporal resolution. allows for monitoring dynamic evolution a protein binding event on millisecond time scale....
Abstract Cesium lead halide perovskites are of interest for light‐emitting diodes and lasers. So far, thin‐films CsPbX 3 have typically afforded very low photoluminescence quantum yields (PL‐QY < 20%) amplified spontaneous emission (ASE) only at cryogenic temperatures, as defect related nonradiative recombination dominated room temperature (RT). There is a current belief that, efficient light from RT, the charge carriers/excitons need to be confined on nanometer scale, like in...
Previous work has shown that the fluorescent styryl dye FM1-43 stains nerve terminals in an activity-dependent fashion. This appears to label membranes of recycled synaptic vesicles by being trapped during endocytosis. Stained can subsequently be destained repeating stimulation absence dye; destaining evidently reflects escape into bathing medium from exocytosing vesicles. In present study we tested two key aspects this interpretation behavior, namely: (i) is localized vesicles, and (ii) it...
We provide a microscopic view of the role halides in controlling anisotropic growth gold nanorods through combined computational and experimental study.
Summary Introduction: This article is part of the Focus Theme Methods Information in Medicine on German Medical Informatics Initiative. “Smart Technology for Healthcare (SMITH)” one four consortia funded by Initiative (MI-I) to create an alliance universities, university hospitals, research institutions and IT companies. SMITH’s goals are establish Data Integration Centers (DICs) at each SMITH partner hospital implement use cases which demonstrate usefulness approach. Objectives: To give...
Efficient and cost-effective multiplexed detection schemes for proteins in small liquid samples would bring drastic advances to fields like disease or water quality monitoring. We present a novel sensor with randomly deposited aptamer functionalized gold nanorods. The spectral position of plasmon resonances individual nanorods, monitored by dark-field spectroscopy, respond specifically different proteins. demonstrate nanomolar sensitivity, recycling, the potential upscale hundreds thousands targets.
Abstract Objective: Associations between body mass index (BMI) and mental disorders meeting Axis‐I diagnoses according to the Diagnostic Statistical Manual for Mental Disorders IV (DSM‐IV) were investigated in The Early Developmental Stages of Psychopathology Study a large population‐based sample, which included adolescents young adults both genders first time. Research Methods Procedures: A total 3021 German subjects ranging from 14 24 years age assessed specific DSM‐IV derived modified...
We used the fluorescence recovery after photobleaching technique to monitor movements of synaptic vesicles in top views living frog motor nerve terminals that had been prestained with fluorescent dye FM1-43. In each experiment, a small portion single stained vesicle cluster was bleached laser and monitored subsequently for signs as neighboring, unbleached moved into region. resting terminals, little or no from occurred. Repetitive stimulation, which caused all spots grow dim released...
Abstract Toxicological effects of nanoparticles are associated with their internalization into cells. Hence, there is a strong need for techniques revealing the interaction between particles and cells as well quantifying uptake at same time. For that reason, herein optical dark‐field microscopy used in conjunction transmission electron to investigate gold epithelial respect shape, stabilizing agent, surface charge. The number internalized strongly dependent on but not particle shape. A test...
Most of current techniques used for the quantification protein–protein interactions require analysis one pair binding partners at a time. Herein we present label-free, simple, fast, and cost-effective route to characterize affinities between multiple macromolecular simultaneously, using optical dark-field spectroscopy individual protein-functionalized gold nanorods as sensing elements. Our NanoSPR method could easily become simple standard tool in biological, biochemical, medical laboratories.
We create rod-shaped single crystalline gold−copper (AuxCu(1-x)) nanoparticles and verify the presence of copper in particles with various direct indirect optical electron microscopy techniques. The grow from small, preformed gold seeds a growth solution containing both ions surfactant mild reducing agent. has pronounced effect on spectral characteristic resulting nanocrystals reduces total volume particles. In contrast to spherical particles, our show strong plasmon resonance content varies...
Lysosomes accumulate many drugs several fold higher compared to their extracellular concentration. This mechanism is believed be responsible for pharmacological effects. So far, uptake and release kinetics are largely unknown interactions between concomitantly administered often provoke mutual interference. In this study, we addressed these questions in a cell culture model. The molecular lysosomal was analyzed by live fluorescence microscopy SY5Y cells using four (amantadine, amitriptyline,...
Electroconvulsive therapy (ECT) is an effective treatment of depression, but its mechanism action still remains unknown. Some studies emphasize that epileptic seizures result in cerebral production cytokines, including the cytokine network association with pathophysiology depression. We hypothesized depressed patients would show a dysregulated profile peripheral cytokines before and after ECT treatment.Fifteen hospitalized subjects major depressive disorder were recruited. Human array IV was...
Heteronanoparticles represent a new class of nanomaterials exhibiting multifunctional and collective properties, which could find applications in medical imaging therapy, catalysis, photovoltaics, electronics. This present work demonstrates the intrinsic heteroepitaxial linkage heterodimer nanoparticles to enable interaction individual components across their interface. It revealed distinct differences between Au@MnO Au@Fe3O4 regarding synthetic procedure growth kinetics, as well properties...
ABSTRACT Dictyostelium discoideummyosin Ik (MyoK) is a novel type of myosin distinguished by remarkable architecture. MyoK related to class I myosins but lacks cargo-binding tail domain and carries an insertion in surface loop suggested modulate motor velocity. This shows similarity secondary actin-binding site present the some myosins, indeed GST-loop construct binds actin. Probably as consequence, binding actin was not only ATP-but also salt-dependent. Moreover, both sites reside within...
Abstract The detailed analysis of β‐amyloid (Aβ) peptides in human plasma is still hampered by the limited sensitivity available mass spectrometric methods and lack appropiate ELISAs to measure Aβ other than 1–38 , 1–40 1–42 . By combining high‐yield immuno precipitation (IP), IEF, urea‐based Aβ‐SDS‐PAGE‐immunoblot, at least 30 Aβ‐immunoreactive spots were detected samples as small 1.6 mL. This approach clearly resolved 1‐42 1‐37 1‐38 1‐39 N‐truncated 2–40 2–42 and, for first time, also...